Control of powdery mildew (Leveillula taurica) using Trichoderma asperellum and Metarhizium anisopliae in different pepper types

López, César Guigón; Muñoz-Castellanos, Laila Nayzzel; Ortiz, Nallely Alejandra Flores; González, Judith Adriana González

doi:10.1007/s10526-018-09916-y

Cited by 17 publications

(2 citation statements)

References 17 publications

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“…Fungal inhibition in dual cultures with Metarhizium species was observed against a variety of other plant pathogens [ 20 , 21 , 22 , 54 ]. Crude extracts and partially purified fractions of Metarhizium have also been reported to inhibit fungal pathogens in previous studies [ 20 , 54 , 55 , 56 ]. Moreover, M. brunneum volatile organic compounds (VOCs) also inhibit plant pathogenic fungi [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Local Competition and Enhanced Defense: How Metarhizium brunneum Inhibits Verticillium longisporum in Oilseed Rape Plants

Posada-Vergara,

Vidal,

Rostás

2023

JoF

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Metarhizium brunneum is a soil-borne fungal entomopathogen that can be associated with plant roots. Previous studies have demonstrated that root colonization by beneficial fungi can directly affect soil-borne pathogens through competition and antibiosis and can activate a systemic response in plants, resulting in a primed state for a faster and/or stronger response to stressors. However, the mechanisms by which Metarhizium inoculation ameliorates symptoms caused by plant pathogens are not well known. This study evaluated the ability of M. brunneum to protect oilseed rape (Brassica napus L.) plants against the soil-borne pathogen Verticillium longisporum and investigated whether the observed effects are a result of direct interaction and/or plant-mediated effects. In vitro and greenhouse experiments were conducted to measure fungal colonization of the rhizosphere and plant tissues, and targeted gene expression analysis was used to evaluate the plant response. The results show that M. brunneum delayed pathogen colonization of plant root tissues, resulting in decreased disease symptoms. Direct competition and antibiosis were found to be part of the mechanisms, as M. brunneum growth was stimulated by the pathogen and inhibited the in vitro growth of V. longisporum. Additionally, M. brunneum changed the plant response to the pathogen by locally activating key defense hormones in the salicylic acid (SA) and abscisic acid (ABA) pathways. Using a split-root setup, it was demonstrated that there is a plant-mediated effect, as improved plant growth and decreased disease symptoms were observed when M. brunneum was in the systemic compartment. Moreover, a stronger systemic induction of the gene PR1 suggested a priming effect, involving the SA pathway. Overall, this study sheds light on the mechanisms underlying the protective effects of M. brunneum against soil-borne pathogens in oilseed rape plants, highlighting the potential of this fungal entomopathogen as a biocontrol agent in sustainable agriculture.

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Section: Discussionmentioning

confidence: 99%

Local Competition and Enhanced Defense: How Metarhizium brunneum Inhibits Verticillium longisporum in Oilseed Rape Plants

Posada-Vergara,

Vidal,

Rostás

2023

JoF

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“…The use of fungicides on a regular basis exposes the environment to chemicals, which can have harmful effects on ecosystems and wildlife (Wightwick et al, 2010). Different genera of bacteria and fungi as biocontrol agents have also been used for controlling the disease (Guigón-López et al, 2018;Abdel-Kader et al, 2012). However, biocontrol agents are not highly effective and require to be combined with other alternatives in integrated management strategies for strong and stable control of pepper powdery mildew (Guigón-López et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

The antifungal effect of MgO and ZnO nanoparticles against powdery mildew disease of pepper (Capsicum annuum L.) under greenhouse conditions

Ismail

2021

Egyptian Journal of Agricultural Research

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Powdery mildew disease of peppers (Capsicum annuum L.) caused by Oidiopsis sicula is the most damaging disease in the field and greenhouse. Chemical control has been widely used for the control of powdery mildew. To minimize the deleterious impacts of fungicides, the present study aimed to evaluate the antifungal effect of different concentrations (100, 150 and 200 mg/L) of MgONPs and ZnONPs against powdery mildew in comparison with the conventional fungicide penconazole (0.25 ml/L) under greenhouse conditions. The synthesis and characterization of MgO and ZnONPs were carried out. Dynamic light scattering (DLS) analysis revealed the presence of MgONPs and ZnONPs at the nanoscale level with a size average of 52.97 nm ± 1.43 S.D and 79.45 nm ± 1.74 S.D, respectively. Two foliar sprays were applied at two-week intervals on pepper plants cv. Dolma, naturally infected with powdery mildew in the three greenhouses of El -Dokki (GH-1), Toukh (GH-2) and El-Haram (GH-3). Both MgONPs and ZnONPs markedly reduced disease severity (DS) and area under the disease progress curve (AUDPC) at a concentration of 200 mg/L. Statistical analysis showed a significant (p<0.05) difference between treatments in the three greenhouses as well as between means of combined treatments. Scanning electron micrographs confirmed that MgONPs and ZnONPs caused inhibition and alterations in the treated fungal structures. Pepper plants treated with penconazole displayed the highest peroxidase activity and recorded 1.229 ΔA422/10 sec/g FW. While, the highest polypheneloxidase activitiy was obtained by MgONPs with value reached 0.283 ΔA495/10 sec/g FW . Out of all treatments, MgONPs were the superior in increasing total chlorophyll at the three applied concentrations, with values of 74.81, 82.94, and 91.19 mg/g FW, respectively. Cytotoxic investigations exhibited the clastogenic nature of MgONPs, ZnONPs, and penconazole. The obtained results are encouraging and emphasize the recognition of MgONPs and ZnONPs as a viable alternatives to conventional strategies.

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Control of Pepper Powdery Mildew Using Antagonistic Microorganisms: An Integral Proposal

López¹,

Ramírez²,

Muñoz-Castellanos

2020

Progress in Biological Control

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Control of powdery mildew (Leveillula taurica) using Trichoderma asperellum and Metarhizium anisopliae in different pepper types

Cited by 17 publications

References 17 publications

Local Competition and Enhanced Defense: How Metarhizium brunneum Inhibits Verticillium longisporum in Oilseed Rape Plants

Local Competition and Enhanced Defense: How Metarhizium brunneum Inhibits Verticillium longisporum in Oilseed Rape Plants

The antifungal effect of MgO and ZnO nanoparticles against powdery mildew disease of pepper (Capsicum annuum L.) under greenhouse conditions

Control of Pepper Powdery Mildew Using Antagonistic Microorganisms: An Integral Proposal

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