Control of osteoblast function and regulation of bone mass

Harada, Satoru; Rodan, Gideon A.

doi:10.1038/nature01660

Cited by 1,268 publications

(972 citation statements)

References 66 publications

Supporting

Mentioning

922

Contrasting

Unclassified

Order By: Relevance

“…Osteoblastic differentiation was substantiated by the expression of RUNX-2, a transcription factor essential for inducing osteoblast differentiation. [13][14][15][16] Cell cycle analysis showed that differentiated cells contained a higher percentage of cells in S phase (38%) with respect to undifferentiated cells. When SBP-DPSCs differentiated into osteoblasts, they gave rise also to endotheliocytes.…”

Section: Resultsmentioning

confidence: 99%

Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation

d’Aquino¹,

Graziano²,

et al. 2007

View full text Add to dashboard Cite

Stromal stem cells from human dental pulp (SBP-DPSCs) were used to study osteogenic differentiation in vitro and in vivo. We previously reported that SBP-DPSCs are multipotent stem cells able to differentiate into osteoblasts, which synthesize threedimensional woven bone tissue chips in vitro. In this study, we followed the temporal expression pattern of specific markers in SBP-DPSCs and found that, when differentiating into osteoblasts, they express, besides osteocalcin, also flk-1 (VEGF-R2). In addition, 30% of them expressed specific antigens for endothelial cells, including CD54, von-Willebrand (domain 1 and 2), CD31 (PECAM-1) and angiotensin-converting enzyme. Interestingly, we found endotheliocytes forming vessel walls, observing that stem cells synergically differentiate into osteoblasts and endotheliocytes, and that flk-1 exerts a pivotal role in coupling osteoblast and endotheliocyte differentiation. When either SBP-DPSCs or bone chips obtained in vitro were transplanted into immunocompromised rats, they generated a tissue structure with an integral blood supply similar to that of human adult bone; in fact, a large number of HLA-1 þ vessels were observed either within the bone or surrounding it in a periosteal layer. This study provides direct evidence to suggest that osteogenesis and angiogenesis mediated by human SBP-DPSCs may be regulated by distinct mechanisms, leading to the organization of adult bone tissue after stem cell transplantion.

show abstract

Section: Resultsmentioning

confidence: 99%

Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation

d’Aquino¹,

Graziano²,

et al. 2007

View full text Add to dashboard Cite

show abstract

“…If this indeed is an important factor, better results would be expected for bone in older subjects that might be more adapted to their loading history. But also in fully grown bone, other factors of biological nature like calcium homeostasis or sex hormones could be physiologically more important than a bone structure perfectly adapted to its loading regime (Beaupre et al 1990;Frost 1987;Harada and Rodan 2003;Manolagas 2000;Robling et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Bone morphology allows estimation of loading history in a murine model of bone adaptation

Christen

Rietbergen

Lambers

et al. 2011

Biomech Model Mechanobiol

View full text Add to dashboard Cite

Bone adapts its morphology (density/microarchitecture) in response to the local loading conditions in such a way that a uniform tissue loading is achieved ('Wolff's law'). This paradigm has been used as a basis for bone remodeling simulations to predict the formation and adaptation of trabecular bone. However, in order to predict bone architectural changes in patients, the physiological external loading conditions, to which the bone was adapted, need to be determined. In the present study, we developed a novel bone loading estimation method to predict such external loading conditions by calculating the loading history that produces the most uniform bone tissue loading. We applied this method to murine caudal vertebrae of two groups that were in vivo loaded by either 0 or 8 N, respectively. Plausible load cases were sequentially applied to micro-finite element models of the mice vertebrae, and scaling factors were calculated for each load case to derive the most uniform tissue strainenergy density when all scaled load cases are applied simultaneously. The bone loading estimation method was able to predict the difference in loading history of the two groups and the correct load magnitude for the loaded group. This result suggests that the bone loading history can be estimated from its morphology and that such a method could be useful for predicting the loading history for bone remodeling studies or at sites where measurements are difficult, as in bone in vivo or fossil bones.

show abstract

“…Remodeling occurs asynchronously at millions of sites throughout the skeleton, regulated primarily by local factors including autocrine/ paracrine mediators and mechanical stimuli. In addition, systemic factors such as parathyroid hormone and estrogen modulate remodeling activity [2]. Under physiological conditions, resorption and formation are tightly coupled, and perturbations to this balance cause bone loss in metabolic diseases such as osteoporosis and inflammatory diseases such as rheumatoid arthritis and periodontitis [3].…”

Section: Introductionmentioning

confidence: 99%

Expression, signaling, and function of P2X7 receptors in bone

Grol

Panupinthu

Korcok

et al. 2009

Purinergic Signalling

View full text Add to dashboard Cite

Nucleotides released from cells in response to mechanical stimulation or injury may serve as paracrine regulators of bone cell function. Extracellular nucleotides bind to multiple subtypes of P2 receptors on osteoblasts (the cells responsible for bone formation) and osteoclasts (cells with the unique ability to resorb mineralized tissues). Both cell lineages express the P2X7 receptor subtype. The skeletal phenotype of mice with targeted disruption of P2rx7 points to interesting roles for this receptor in the regulation of bone formation and resorption, as well as the response of the skeleton to mechanical stimulation. This paper reviews recent work on the expression of P2X7 receptors in bone, their associated signal transduction mechanisms and roles in regulating bone formation and resorption. Areas for future research in this field are also discussed.

show abstract

Control of osteoblast function and regulation of bone mass

Cited by 1,268 publications

References 66 publications

Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation

Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation

Bone morphology allows estimation of loading history in a murine model of bone adaptation

Expression, signaling, and function of P2X7 receptors in bone

Contact Info

Product

Resources

About