Control of Organogenesis and Embryogenesis in Rice Calli.

Yoshida, Kaoru; Fujii, Satoshi; Sakata, Makoto; Takeda, Genkichi

doi:10.1270/jsbbs1951.44.355

Cited by 21 publications

(12 citation statements)

References 24 publications

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“…Also, exogenously applied proline in a two-step culture method drastically increased somatic embryo formation, maturation and subsequently plant regeneration in Paspalum scorbiculatum L. (Ceasar and Ignasimuthu 2010). Also, the promotive effect of proline on androgenesis induction has been reported in many crop species (Yoshida et al 1994;Redha et al 1998;Hema and Murthy 2008). Significantly higher embryos were obtained from in vitro cultured anthers of niger when proline was added to the induction medium (Hema and Murthy 2008).…”

Section: Introductionmentioning

confidence: 91%

Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassica napus L.

Ahmadi

Shariatpanahi

2015

Plant Cell Tiss Organ Cult

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The effects of proline (0, 50, 100, 200 and 500 mg l -1 ) and chitosan (0, 10, 20, 50 and 100 mg l -1 ) treatment at three incubation periods (1, 2 and 5 days) were assessed on the efficiency of microsopore embryogenesis induction and subsequently plantlet regeneration in rapeseed (Brassica napus L.) cv. ''Hyola 401''. About 30-55 % increase in microspore embryogenesis was observed in the cultures exposed to 100 mg l -1 proline for 2 and 5 days. Higher levels were not advantageous so that, microspore embryogenesis significantly reduced when 200 and 500 mg l -1 proline were exogenously applied to the culture medium. High normal regeneration was achieved in the cultures treated with 50 and 100 mg l -1 proline for 2 and 5 days. In addition, callogenesis increased as proline level and duration of its treatment was increased in which the majority of microspore-derived embryos (78 %) underwent callusing in the cultures exposed to 500 mg l -1 proline for 5 days. In contrast to untreated cultures, the efficiency of microspore embryogenesis increased about 1.8-fold in response to 10 mg l -1 chitosan for 2 days. High levels of chitosan were detrimental to microspore embryogenesis so that embryogenesis was completely inhibited in the cultures exposed to 50 and 100 mg l -1 for 5 days. Chitosan treatment was not advantageous to the normal plantlet development at all levels and durations tested. High levels and durations of chitosan treatment resulted in the higher callusing so that the highest callusing (88 and 79 %) were observed in the presence of 100 mg l -1 for 1 day and 20 mg l -1 for 2 days, respectively. Efficiency of microspore embryogenesis induction and plantlet regeneration could be improved by proline and chitosan treatment when appropriate levels and durations of incubation were selected.

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Section: Introductionmentioning

confidence: 91%

Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassica napus L.

Ahmadi

Shariatpanahi

2015

Plant Cell Tiss Organ Cult

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“…The initiation of callus suspension cultures from the scutellum of mature seeds and the induction of somatic embryogenesis and organogenesis (regeneration) have been described previously (Yoshida et al, 1994a).…”

Section: Plant Material Cell Cultures and Cdna Clonesmentioning

confidence: 99%

“…During the course of our research to isolate genes that may play a role in embryogenesis in rice (Yoshida et al, 1994a(Yoshida et al, , 1994bMizobuchi-Fukuoka et al, 1996), we isolated cDNA clones that were preferentially expressed in calli in which somatic embryogenesis was induced (MizobuchiFukuoka et al, 1996). In the present study we analyzed one of the clones, pRINO1, that showed a high homology to I(1)P synthase from yeast and plants.…”

mentioning

confidence: 99%

Temporal and Spatial Patterns of Accumulation of the Transcript of Myo-Inositol-1-Phosphate Synthase and Phytin-Containing Particles during Seed Development in Rice1

et al. 1999

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Myo-inositol-1-phosphate (I[1]P) synthase (EC 5.5.1.4) catalyzes the reaction from glucose 6-phosphate to I(1)P, the first step of myo-inositol biosynthesis. Among the metabolites of I(1)P is inositol hexakisphosphate, which forms a mixed salt called phytin or phytate, a storage form of phosphate and cations in seeds. We have isolated a rice (Oryza sativa L.) cDNA clone, pRINO1, that is highly homologous to the I(1)P synthase from yeast and plants. Northern analysis of total RNA showed that the transcript accumulated to high levels in embryos but was undetectable in shoots, roots, and flowers. In situ hybridization of developing seeds showed that the transcript first appeared in the apical region of globular-stage embryos 2 d after anthesis (DAA). Strong signals were detected in the scutellum and aleurone layer after 4 DAA. The level of the transcript in these cells increased until 7 DAA, after which time it gradually decreased. Phytin-containing particles called globoids appeared 4 DAA in the scutellum and aleurone layer, coinciding with the localization of the RINO1 transcript. The temporal and spatial patterns of accumulation of the RINO1 transcript and globoids suggest that I(1)P synthase directs phytin biosynthesis in rice seeds.Myo-inositol and I(1)P are essential for the survival of eukaryotic cells, because various metabolic routes diverge from these two compounds. A number of possible roles for myo-inositol and I(1)P metabolites in plant development have been demonstrated (for review, see Bohnert et al., 1995). I(1)P is utilized for the synthesis of plasma membrane phosphoinositides, which are involved in signal transduction as sources of second messengers (Gross and Boss, 1993). Myo-inositol combined with Gal is incorporated into the raffinose family of the vegetative storage form of carbohydrates (Kandler and Hopf, 1982). Oxidized inositols serve as noncellulosic cell wall components (Loewus and Loewus, 1983), and methylated inositols were shown to be involved in osmoprotection in a halophytic plant (Ishitani et al., 1996). In addition, I(1)P is further phosphorylated to inositol hexakisphosphoric acid, or phytic acid, which strongly binds metallic cations such as K, Mg, Mn, Ca, Fe, and Zn via ionic associations with negatively charged phosphates (Maga, 1982;Lott et al., 1995) to form a mixed salt called phytin or phytate (Ashton, 1976). The synthesis of phytin as a storage form of phosphate and cations is a metabolic pathway unique to plants (Loewus and Dickinson, 1982).Phytin is mainly stored in protein bodies in seeds as spherical inclusions called globoids (Lott et al., 1995). Cereal grains and oil seeds are particularly rich sources of phytin, and the phosphorus of phytin represents 80% to 90% of total phosphorus in these seeds (Maga, 1982). In rice (Oryza sativa) seeds phytin accumulates in the aleurone and scutellum cells but not in the starchy endosperm cells (Tanaka et al., 1973;Ogawa et al., 1977). During germination phytin is digested by an acid phosphatase called phytase (Laboure et al....

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“…One-month-old calli were then transferred to suspension culture and maintained in R2 medium (Ohira et al, 1973) supplemented with 2,4-D at 2 mg/l. Total RNA was isolated from 8-week-old calli which were subcultured every week (Yoshida et al, 1994a). The SDS-phenol method employed by Watanabe and Price (1982) was applied for RNA extraction, and RNA was precipitated with lithium chloride.…”

Section: Methodsmentioning

confidence: 99%

“…We employed the callus induced from the seeds of Yamahoushi (Japonica), because Yamahoushi has a superior performance for callus proliferation (Yoshida et al, 1994a) and is genetically close to Nipponbare, which shares the same EPSPs-rps20 genomic segment. The 3V RACE products that had different sizes from the cDNA clones screened from the callus library comprised 22 clones for EPSPs and 9 clones for rps20.…”

Section: Transcriptional Termination Of the Two Genes In Rice Callusmentioning

confidence: 99%

Evidence for an evolutionary force that prevents epigenetic silencing between tail-to-tail rice genes with a short spacer

Kobayashi

Noro

Nagano

et al. 2005

Gene

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Control of Organogenesis and Embryogenesis in Rice Calli.

Cited by 21 publications

References 24 publications

Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassica napus L.

Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassica napus L.

Temporal and Spatial Patterns of Accumulation of the Transcript of Myo-Inositol-1-Phosphate Synthase and Phytin-Containing Particles during Seed Development in Rice1

Evidence for an evolutionary force that prevents epigenetic silencing between tail-to-tail rice genes with a short spacer

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