Control of Organelle Size: The Golgi Complex

Sengupta, Debapriya; Linstedt, Adam D.

doi:10.1146/annurev-cellbio-100109-104003

Cited by 75 publications

(82 citation statements)

References 158 publications

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“…7). In addition, the number of GM130 puncta overlapping with Arm was greatly increased in klp64D k1 mutant embryos and wing discs, which might be due to an increased processing demand in Golgi (Sengupta and Linstedt, 2011).…”

Section: Discussionmentioning

confidence: 98%

Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila

Vuong

Mukhopadhyay²,

Choi

2014

Development

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Wingless (Wg)/Wnt signaling is fundamental in metazoan development. Armadillo (Arm)/β-catenin and Dishevelled (Dsh) are key components of Wnt signal transduction. Recent studies suggest that intracellular trafficking of Wnt signaling components is important, but underlying mechanisms are not well known. Here, we show that Klp64D, the Drosophila homolog of Kif3A kinesin II subunit, is required for Wg signaling by regulating Arm during wing development. Mutations in klp64D or RNAi cause wing notching and loss of Wg target gene expression. The wing notching phenotype by Klp64D knockdown is suppressed by activated Arm but not by Dsh, suggesting that Klp64D is required for Arm function. Furthermore, klp64D and arm mutants show synergistic genetic interaction. Consistent with this genetic interaction, Klp64D directly binds to the Arm repeat domain of Arm and can recruit Dsh in the presence of Arm. Overexpression of Klp64D mutated in the motor domain causes dominant wing notching, indicating the importance of the motor activity. Klp64D shows subcellular localization to intracellular vesicles overlapping with Arm and Dsh. In klp64D mutants, Arm is abnormally accumulated in vesicular structures including Golgi, suggesting that intracellular trafficking of Arm is affected. Human KIF3A can also bind β-catenin and rescue klp64D RNAi phenotypes. Taken together, we propose that Klp64D is essential for Wg signaling by trafficking of Arm via the formation of a conserved complex with Arm.

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Section: Discussionmentioning

confidence: 98%

Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila

Vuong

Mukhopadhyay²,

Choi

2014

Development

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“…49,50 Below 20 C membrane viscosity is changed, endocytosis is impaired, degradation is delayed due to defective transport to the lysosome 51 and secretion is also delayed likely because of budding defect at the Golgi generating increased cargo load. 35,52 Thus, we envision this temperature-dependent difference as a consequence of both modified GSL contents and accumulated protein cargoes (i.e. GPI-anchored proteins) in VAMP7 KD and KO cells.…”

Section: Discussionmentioning

confidence: 99%

Role of tetanus neurotoxin insensitive vesicle-associated membrane protein in membrane domains transport and homeostasis

et al. 2015

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Keywords: exocytosis, Golgi apparatus, SNARE, sphingolipids, TI-VAMP/VAMP7 Abbreviations: BFA, Brefeldin A; Cer, Ceramide; ER, Endoplasmic Reticulum; GlcCer, Glucosylceramide; GM3, ganglioside monosialic acid 3; GPI, Glycosylphosphatidylinositol; GSL, Glycosphingolipids; LC, Long Chain; PI, Phosphatidylinositide; PM, Plasma Membrane; SM, Sphingomyelin; TGN, D Trans-Golgi Network; TI-VAMP/VAMP7, Tetanus neurotoxin-insensitive vesicle-associated membrane protein / Vesicle associated membrane protein 7; VLC, very long vhain; VSVG, Vesicular Stomatitis Virus GlycoproteinBiological membranes in eukaryotes contain a large variety of proteins and lipids often distributed in domains in plasma membrane and endomembranes. Molecular mechanisms responsible for the transport and the organization of these membrane domains along the secretory pathway still remain elusive. Here we show that vesicular SNARE TI-VAMP/VAMP7 plays a major role in membrane domains composition and transport. We found that the transport of exogenous and endogenous GPI-anchored proteins was altered in fibroblasts isolated from VAMP7-knockout mice. Furthermore, disassembly and reformation of the Golgi apparatus induced by Brefeldin A treatment and washout were impaired in VAMP7-depleted cells, suggesting that loss of VAMP7 expression alters biochemical properties and dynamics of the Golgi apparatus. In addition, lipid profiles from these knockout cells indicated a defect in glycosphingolipids homeostasis. We conclude that VAMP7 is required for effective transport of GPI-anchored proteins to cell surface and that VAMP7-dependent transport contributes to both sphingolipids and Golgi homeostasis.

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“…Golgi elements directly interact with microtubule motors, dynein and kinesin. The interaction of the Golgi with the dynein1 complex is critical for both compaction of the Golgi and maintenance of Golgi stacks (27). Disruption of directional Golgi orientation by loss of golgins, golgin-160 and GMAP210, impairs directional cell migration and wound healing, demonstrating a direct relationship between polarization of Golgi stacks and directional migration (28).…”

Section: Discussionmentioning

confidence: 99%

Rotational motion during three-dimensional morphogenesis of mammary epithelial acini relates to laminin matrix assembly

Wang

Lacoche

Huang

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

118

107

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Our understanding of the mechanisms by which ducts and lobules develop is derived from model organisms and three-dimensional (3D) cell culture models wherein mammalian epithelial cells undergo morphogenesis to form multicellular spheres with a hollow central lumen. However, the mechanophysical properties associated with epithelial morphogenesis are poorly understood. We performed multidimensional live-cell imaging analysis to track the morphogenetic process starting from a single cell to the development of a multicellular, spherical structure composed of polarized epithelial cells surrounding a hollow lumen. We report that in addition to actively maintaining apicobasal polarity, the structures underwent rotational motions at rates of 15-20 μm/h and the structures rotated 360°every 4 h during the early phase of morphogenesis. Rotational motion was independent of the cell cycle, but was blocked by loss of the epithelial polarity proteins Scribble or Pard3, or by inhibition of dynein-based microtubule motors. Interestingly, none of the structures derived from human cancer underwent rotational motion. We found a direct relationship between rotational motion and assembly of endogenous basement membrane matrix around the 3D structures, and that structures that failed to rotate were defective in weaving exogenous laminin matrix. Dissolution of basement membrane around mature, nonrotating acini restored rotational movement and the ability to assemble exogenous laminin. Thus, coordinated rotational movement is a unique mechanophysical process observed during normal 3D morphogenesis that regulates laminin matrix assembly and is lost in cancer-derived epithelial cells. extracellular matrix | tubulogenesis | plasticity T issues are composed of a community of cells that act in a coordinated manner to achieve and maintain normal architecture and perform their function. The mechanisms by which cells coordinate their behavior during tissue morphogenesis is poorly understood in part due to technical bottlenecks associated with studying the process in vivo. Three-dimensional (3D) culture systems provide an environment in which normal tissue morphogenesis can be recapitulated and thus is a powerful tool for investigating the molecular signals that specify epithelial tissue architecture (1-3). Unlike monolayer cultures, epithelial cells grown in 3D recapitulate numerous features seen in vivo, including the formation of acini-like spheroids with a hollow lumen, apicobasal polarization of cells comprising these acini, and the deposition of basement membrane components collagen IV and laminin-332 (2).The initial stages of 3D morphogenesis are characterized by the ability of epithelial cells to detect surfaces with which they come in contact. Cells use integrins and dystroglycans to contact the extracellular matrix (ECM), and cadherins and desmosomes to contact their neighbors (3). These initial contacts trigger a series of cell remodeling events, which result in polarization of cells to create a basolateral surface and an apical surfa...

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Control of Organelle Size: The Golgi Complex

Cited by 75 publications

References 158 publications

Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila

Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila

Role of tetanus neurotoxin insensitive vesicle-associated membrane protein in membrane domains transport and homeostasis

Rotational motion during three-dimensional morphogenesis of mammary epithelial acini relates to laminin matrix assembly

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