Control of one Stage Bio Ethanol Production by Recombinant Strain

Abstract

A new method for adaptive control of simultaneous saccharifi cation and fermentation of starch to ethanol by the recombinant strain Saccharomyces cerevisiae YPB-G is proposed. The process monitoring is enriched by new software sensors of glucose consumption and production rates. The difference between their values is defi ned as a control marker which is used for switching from batch to fed-batch mode automatically and for determining the amplitude and duration of starch feeding pulses (control input). Simulation results have shown that the proposed control strategy stabilizes the process at an equilibrium state for the glucose concentration. In this way, the ethanol concentration in the reactor and the productivity of the process are increased.

Control of α-amylase production by Bacillus subtilis

“…An optimal tuning procedure for the software sensors was proposed on the basis of the properties of the process stability and measurement error. This method has been applied to polyb-hydroxybutyrate production by a mixed culture [12,13], bioethanol production by a recombinant strain [14] and gluconic acid production [11] control.…”

“…The main disadvantage of open-loop control is the lack of online information about the real physiological state of the process. Therefore, nowadays closed-loop algorithms are mainly developed [11][12][13][14][15][16] where the online information of one or more process variables can be used. Difficulties may cause the lack of reliable sensors for biomass, and for certain metabolites and substrates.…”