Control of normal differentiation of myeloid leukemic cells. V. Normal differentiation in aneuploid leukemic cells and the chromosome banding pattern of D<sup>+</sup> and D<sup>−</sup> clones

Hayashi, Makoto; Fibach, Eitan; Sachs, Leo

doi:10.1002/ijc.2910140106

Cited by 33 publications

(11 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have shown that some, but not all, undifferentiated mammalian myeloid leukemic cells can be induced to undergo differentiation to mature macrophages and granulocytes (1,2) and had found two types of clones. One type (D +) could be induced to undergo cell differentiation, and the other type (D -) could not be induced to differentiate to mature cells (3)(4)(5)(6)(7).…”

mentioning

confidence: 99%

Different Blocks in the Differentiation of Myeloid Leukemic Cells

Lotem

Sachs

1974

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

170

View full text Add to dashboard Cite

Cells. Ten clones of myeloid leukemic cells were used. Three D+(nos. 9, 11, and 21) and three D-(nos. 5, 13, and 16) clones were isolated and clone-purified three times (3) from a myeloid leukemic cell line growing in culture obtained from an SL mouse with myeloid leukemia (10). The other four clones were four myeloid leukemic cell lines (1-4) (Sukster, Fibach, and Sachs, to be published) derived from four independently arising myeloid leukemias in SJL/J mice (11). The cells of all 10 lines produced leukemia after inoculation into adult animals and grew in suspension in liquid medium as myeloblasts. Cells were cultured in Eagle's medium with a 4-fold concentration of amino acids and vitamins and 10% (v/v) inactivated (560 for 30 min) horse or fetal-calf serum. The same results were obtained with both types of serum. In order to obtain the same concentration of cells for determinations of the percent of cells with receptors at 1, 2, 3, and 4 days after seeding, cells were seeded at 2.4, 1.2, 0.6, and 0.3 X 101 cells, respectively, in 5 ml of medium per 50-mm petri dish. The cells seeded at these four concentrations had the same growth rate. Normal mature macrophages and granulocytes were collected from the peritoneal cavity of mice 4 days after intraperitoneal injection of thioglycollate medium and 4 hr after intraperitoneal injection of 1% glycogen solution, respectively.The cells were washed three times with phosphate-buffered saline, pH 7.2, and suspended in Eagle's medium without bicarbonate, whose pH was adjusted to 7.0 with NaOH, before they were tested for surface receptors.Sensitization of Sheep Erythrocytes with Antibody and Complement. The erythrocytes were sensitized as described (12). Sheep erythrocytes stored at 4°in Alsevers solution were washed three times with phosphate-buffered saline and suspended in phosphate-buffered saline to give a 0.5% (v/v) suspension. An equal volume of 1:1500 dilution of rabbit antiserum directed against sheep erythrocytes was added, and the mixture was incubated for 30 min at 37°. The erythrocytes sensitized with antibody (EA) were washed three times with phosphate-buffered saline and suspended at 0.5% (v/v) inEagle's medium without bicarbonate, pH 7.0. Erythrocytes sensitized with antibody and complement (EAC) were ob-

show abstract

mentioning

confidence: 99%

Different Blocks in the Differentiation of Myeloid Leukemic Cells

Lotem

Sachs

1974

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

170

View full text Add to dashboard Cite

show abstract

“…We have addressed this question by using a whole-genome expression approach and compared gene expression in the cloned mouse myeloid leukemic cell line M1-t-p53 (20) and in 45 normal mouse tissues (21). A study of the chromosomes of M1 myeloid leukemic clones has shown that Ͼ90% of the cells have the same chromosome number, and all of the cells in a clone have the same chromosome abnormalities (10). The growth curve of M1-t-p53 cells has shown that these cells have a high rate of cell multiplication, and Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The selected genes were filtered according to their expression in the 45 normal mouse tissues to remove those genes that show a similar expression level in all 45 tissues. We used two criteria to filter the genes: (i) genes whose expression showed a high SD in the different mouse tissues, Ն0.3 of their log 10 -transformed expression values, and (ii) genes whose expression level range was Ն1 in log 10 transformed values and whose expression in at least one tissue Abbreviations: SPC, super paramagnetic clustering; TG, thapsigargin. ‡ To whom correspondence should be addressed.…”

Section: Methodsmentioning

confidence: 99%

“…In addition to the ability for self-renewal, normal hematopoietic stem cells thus have transcription accessibility for genes expressed in nonhematopoietic tissues, and this result can explain the plasticity of these normal stem cells (4)(5)(6). Studies on the chromosomes (7-10) and other properties (11,12) of cancer cells have shown that cancers also have self-renewing stem cells (7)(8)(9)(10)(11)(12). It was, therefore, of interest to determine whether cancer stem cells have the ability to express genes that are expressed in different normal tissues and how expression of such genes can be regulated under different conditions.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Induction in myeloid leukemic cells of genes that are expressed in different normal tissues

Lotem¹,

Benjamin²,

Netanely³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Using DNA microarray and cluster analysis of expressed genes in a cloned line (M1-t-p53) of myeloid leukemic cells, we have analyzed the expression of genes that are preferentially expressed in different normal tissues. Clustering of 547 highly expressed genes in these leukemic cells showed 38 genes preferentially expressed in normal hematopoietic tissues and 122 other genes preferentially expressed in different normal nonhematopoietic tissues, including neuronal tissues, muscle, liver, and testis. We have also analyzed the genes whose expression in the leukemic cells changed after activation of WT p53 and treatment with the cytokine IL-6 or the calcium mobilizer thapsigargin. Of 620 such genes in the leukemic cells that were differentially expressed in normal tissues, clustering showed 80 genes that were preferentially expressed in hematopoietic tissues and 132 genes in different normal nonhematopietic tissues that also included neuronal tissues, muscle, liver, and testis. Activation of p53 and treatment with IL-6 or thapsigargin induced different changes in the genes preferentially expressed in these normal tissues. These myeloid leukemic cells thus express genes that are expressed in normal nonhematopoietic tissues, and various treatments can reprogram these cells to induce other such nonhematopoietic genes. The results indicate that these leukemic cells share with normal hematopoietic stem cells the plasticity of differentiation to different cell types. It is suggested that this reprogramming to induce in malignant cells genes that are expressed in different normal tissues may be of clinical value in therapy.

show abstract

“…Induction of cell migration, attachment to the surface of a tissue culture Petri Fibach, E., and L. Sachs 1974 Control of nordish and h e formation of macrophages, mal differentiation Of myeloid leukemic cells. IV.…”

Section: Discussionmentioning

confidence: 99%

Control of normal differentiation of myeloid leukemic cells. VI. Inhibition of cell multiplication and the formation of macrophages

Lotem

Sachs

1975

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

D+ but not D- myeloid leukemic cells can be induced by the appropriate conditioned medium or by serum from endotoxin treated mice, to undergo cell migration in agar, cell attachment to the surface of a Petri dish and differentiation to mature macrophages and granulocytes. Inhibition of cell multiplication by cytosine arabinoside, hydroxyurea, mitomycin C, thymidine, 5-bromodeoxyuridine, 5-iododeoxyuridine, 5-fluorodeoxyuridine or actinomycin D, but not by vinblastine or cycloheximide, induced cell migration, cell attachment to the Petri dish and the formation of macrophages in D+ cells. There was no induction of cell migration or formation of macrophages and a much lower induction of cell attachment in D- cells. The induction of these changes in D+ cells required protein synthesis and the inhibitors showed the same toxicity for D+ and D- cells. The results indicate, that the inhibitors induced specific surface membrane changes in D+ but not in D- cells.

show abstract

Control of normal differentiation of myeloid leukemic cells. V. Normal differentiation in aneuploid leukemic cells and the chromosome banding pattern of D⁺ and D⁻ clones

Cited by 33 publications

References 21 publications

Different Blocks in the Differentiation of Myeloid Leukemic Cells

Different Blocks in the Differentiation of Myeloid Leukemic Cells

Induction in myeloid leukemic cells of genes that are expressed in different normal tissues

Control of normal differentiation of myeloid leukemic cells. VI. Inhibition of cell multiplication and the formation of macrophages

Contact Info

Product

Resources

About

Control of normal differentiation of myeloid leukemic cells. V. Normal differentiation in aneuploid leukemic cells and the chromosome banding pattern of D+ and D− clones

Cited by 33 publications

References 21 publications

Different Blocks in the Differentiation of Myeloid Leukemic Cells

Different Blocks in the Differentiation of Myeloid Leukemic Cells

Induction in myeloid leukemic cells of genes that are expressed in different normal tissues

Control of normal differentiation of myeloid leukemic cells. VI. Inhibition of cell multiplication and the formation of macrophages

Contact Info

Product

Resources

About

Control of normal differentiation of myeloid leukemic cells. V. Normal differentiation in aneuploid leukemic cells and the chromosome banding pattern of D⁺ and D⁻ clones