Control of Membrane Morphogenesis in Bacteriophage

Brewer, Gregory J.

doi:10.1016/s0074-7696(08)62307-4

Cited by 15 publications

(6 citation statements)

References 107 publications

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“…However, to achieve the 2.6-fold enrichment of viral phosphatidylglycerol and twofold depletion of phosphatidylethanolamine (20), a specific selection of phosphatidylglycerol from the host membrane seemed to be required. These results are in concert with a molecular model for morphogenesis of the membrane of PM2 proposed previously (4).…”

Section: Downloaded Fromsupporting

confidence: 88%

“…Here we report tests of this prediction by analysis of the reactivity of phosphatidylethanolamine in the host cytoplasmic membrane with 2,4,6-trinitrobenzenesulfonic acid (TNBS). These results have been summarized previously (4).…”

mentioning

confidence: 58%

“…During infection, numerous virus-sized membranes are assembled inside the cell before cell lysis. Bacteriophage PM2 matures at the inner face of the cytoplasmic membrane of its host (1,4,7,8). Although not readily observed in electron micrographs of infected cells, examples have been found of putative viral membranes formed by an inward budding or invagination of the cytoplasmic membrane of the host bacterium (4).…”

mentioning

confidence: 99%

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Accessibility of phosphatidylethanolamine in bacteriophage PM2 and in its gram-negative host

Brewer¹,

Goto²

1983

J Virol

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confidence: 88%

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confidence: 58%

mentioning

confidence: 99%

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Accessibility of phosphatidylethanolamine in bacteriophage PM2 and in its gram-negative host

Brewer¹,

Goto²

1983

J Virol

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“…In prokaryotic viruses, however, no genes encoding components for lipid metabolism have been recognized, but the membranes of prokaryotic viruses are mostly obtained from the host cytoplasmic membranes [13, 14]. Enveloped viruses obtain the membrane during budding, that is, egress of the viral particles from the cells without disturbing the cell membrane integrity [15].…”

Section: Introductionmentioning

confidence: 99%

Lipids of Archaeal Viruses

Roine

Bamford

2012

Archaea

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Archaeal viruses represent one of the least known territory of the viral universe and even less is known about their lipids. Based on the current knowledge, however, it seems that, as in other viruses, archaeal viral lipids are mostly incorporated into membranes that reside either as outer envelopes or membranes inside an icosahedral capsid. Mechanisms for the membrane acquisition seem to be similar to those of viruses infecting other host organisms. There are indications that also some proteins of archaeal viruses are lipid modified. Further studies on the characterization of lipids in archaeal viruses as well as on their role in virion assembly and infectivity require not only highly purified viral material but also, for example, constant evaluation of the adaptability of emerging technologies for their analysis. Biological membranes contain proteins and membranes of archaeal viruses are not an exception. Archaeal viruses as relatively simple systems can be used as excellent tools for studying the lipid protein interactions in archaeal membranes.

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“…Studies of lipid-containing bacterial viruses have yielded considerable information on the structure and assembly of viral membranes (1,7,12,35,36,42,49). Crucial for this progress has been the availability of defined genetic systems (39,47).…”

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confidence: 99%

Genome Characterization of Lipid-Containing Marine Bacteriophage PM2 by Transposon Insertion Mutagenesis

Krupovìč

Vilen

Bamford

et al. 2006

J Virol

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Bacteriophage PM2 presently is the only member of the Corticoviridae family. The virion consists of a protein-rich lipid vesicle, which is surrounded by an icosahedral protein capsid. The lipid vesicle encloses a supercoiled circular double-stranded DNA genome of 10,079 bp. PM2 belongs to the marine phage community and is known to infect two gram-negative Pseudoalteromonas species. In this study, we present a characterization of the PM2 genome made using the in vitro transposon insertion mutagenesis approach. Analysis of 101 insertion mutants yielded information on the essential and dispensable regions of the PM2 genome and led to the identification of several new genes. A number of lysis-deficient mutants as well as mutants displaying delayed-and/or incomplete-lysis phenotypes were identified. This enabled us to identify novel lysis-associated genes with no resemblance to those previously described from other bacteriophage systems. Nonessential genome regions are discussed in the context of PM2 genome evolution.

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Control of Membrane Morphogenesis in Bacteriophage

Cited by 15 publications

References 107 publications

Accessibility of phosphatidylethanolamine in bacteriophage PM2 and in its gram-negative host

Accessibility of phosphatidylethanolamine in bacteriophage PM2 and in its gram-negative host

Lipids of Archaeal Viruses

Genome Characterization of Lipid-Containing Marine Bacteriophage PM2 by Transposon Insertion Mutagenesis

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