Control of lymphocyte stimulation in vitro: ‘help’ and ‘suppression’ in the light of lymphoid population dynamics

Knight, Stella C.

doi:10.1016/0022-1759(82)90297-6

Cited by 33 publications

(6 citation statements)

References 33 publications

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“…Lymphocyte transformation responses to PHA were carried out using standard methods 9. This involved measurement of maximal incorporation of tritiated thymidine into stimulated peripheral blood mononuclear cells after 4 days of culture.…”

Section: Methodsmentioning

confidence: 99%

Association between hypoparathyroidism and defective T cell immunity in 22q11.2 deletion syndrome

et al. 2010

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22q11.2 syndrome patients with hypoparathyroidism are more likely to have a clinically significant T cell immunodeficiency and lower laboratory parameters of T cell function, with a higher risk of thrush and viral bronchiolitis and enteritis. Measurement of absolute CD3 count is a simple and accurate predictor of fungal/viral infection risk, with phytohaemagglutinin mitogen responses providing little or no further value in most patients.

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Section: Methodsmentioning

confidence: 99%

Association between hypoparathyroidism and defective T cell immunity in 22q11.2 deletion syndrome

et al. 2010

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“…IL-12, TNF-α) (Larsen et al, 1992;Lechler et al, 2001). Therefore, mature DCs induce the helper T and cytotoxic T cell responses both in vitro (Knight 1982;Macatonia et al, 1989;Steinman 1991) and in vivo (Inaba et al, 1990;Sornasse et al, 1992). In addition, several reports have shown that the increased DCs-derived IL-12 favored a Th1 function (Wang et al, 2002;Braat et al, 2003;Byl et al, 2003;Flohe et al, 2003;Hou et al, 2003a).…”

Section: Introductionmentioning

confidence: 99%

Morin Promotes the Production of Th2 Cytokine by Modulating Bone Marrow-Derived Dendritic Cells

Suen

Chiang

et al. 2006

Am. J. Chin. Med.

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Our previous studies had reported that morin decreased the interleukin-12 (IL-12) and tumor necrosis factor-alpha (TNF-alpha) production in lipopolysaccharide (LPS)-activated macrophages, suggesting that morin may promote helper T type 2 (Th2) response in vivo. Dendritic cells (DCs) are the most potent antigen presenting cells and known to play a major role in the differentiation of helper T type 1 (Th1) and Th2 responses. This study aimed to reveal whether morin is able to control the Th differentiation through modulating the maturation and functions of DCs. Bone marrow-derived dendritic cells (BM-DCs) were incubated with various concentrations of morin and their characteristics were studied. The results indicated that morin significantly affects the phenotype and cytokine expression of BM-DCs. Morin reduced the production of IL-12 and TNF-alpha in BM-DCs, in response to LPS stimulation. In addition, the proliferative response of stimulated alloreactive T cells was significantly decreased by morin in BM-DCs. Furthermore, allogeneic T cells secreted higher IL-4 and lower IFN-gamma in response to morin in BM-DCs. In conclusion, these results suggested that morin favors Th2 cell differentiation through modulating the maturation and function of BM-DCs.

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“…The development history of MLC has been characterized by a trend toward miniaturisation and early detection of responses (Thurman et al 1973, Mawas et al 1976, Knight & Burman 1981, Wank 1982. As far as we are aware the use of Terasaki 60-well tissue-typing trays for MLC was first described -by Pena-Martinez & Festenstein (1975).…”

Section: Discussionmentioning

confidence: 99%

“…A more detailed analysis of responses is possible with HD MLC than with conventional (96-well plate) MLC, using the same number of cells. Consequently it has been possible to examine several kinetic parameters simultaneously (Knight 1982). Technical considerations are unlikely to effect 3H-Tdr uptake since the time-course kinetics of H D MLC are similar to conventional MLC (Bondevik et al 1974, JGrgensen & Lamm 1974, Osoba & Falk 1974.…”

Section: Discussionmentioning

confidence: 99%

Optimum cell numbers for mixed lymphocyte responses in hanging‐drop microcultures

et al. 1984

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The conditions required for optimum mixed lymphocyte culture (MLC) responses in hanging-drop (HD) microcultures in Terasaki trays have been investigated. Compared with conventional (200 microliter) MLC, HD-MLC required 5-10 times fewer cells, and 10% of the amount of culture medium and 3H-thymidine label. Peak uptake of label in HD-MLC was mutually responder-stimulator concentration dependent. Differences in response to related and unrelated lymphocytes indicate that HLA-D determinants are detected in HD-MLC.

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Control of lymphocyte stimulation in vitro: ‘help’ and ‘suppression’ in the light of lymphoid population dynamics

Cited by 33 publications

References 33 publications

Association between hypoparathyroidism and defective T cell immunity in 22q11.2 deletion syndrome

Association between hypoparathyroidism and defective T cell immunity in 22q11.2 deletion syndrome

Morin Promotes the Production of Th2 Cytokine by Modulating Bone Marrow-Derived Dendritic Cells

Optimum cell numbers for mixed lymphocyte responses in hanging‐drop microcultures

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