Control of hemopoiesis by a bone marrow stromal cell clone: lipopolysaccharide- and interleukin-1-inducible production of colony- stimulating factors

Abstract

A stromal cell line, GY30, was cloned from mouse bone marrow adherent cell layers. In culture, GY30 cells sustain the production of granulocyte-macrophage progenitor cells (GM-CFU) but fail to support the survival of pluripotential stem cells (CFU-S). GY30 cells secrete two growth factor activities distinct from interleukin-3 (IL-3), IL-2, and macrophage colony-stimulating factor (M-CSF) but functionally similar to GM-CSF and G-CSF. The production of both CSFs is increased 70- to 200-fold by treating GY30 cells with lipopolysaccharide or IL-1. RNA blot analysis reveals the presence of GM-CSF and G-CSF transcripts and demonstrates that IL-1 regulates the production of both factors at the mRNA level. Further, these studies show that the GM-CSF secreted by GY30 cells is structurally similar to the GM-CSF produced by activated T cells.

Siglec‐F is induced by granulocyte–macrophage colony‐stimulating factor and enhances interleukin‐4‐induced expression of arginase‐1 in mouse macrophages

“…37,38 A number of different cell types including T lymphocytes, macrophages, and cancer cells produce GM-CSF. 37,40 In most cases, inflammatory stimuli induce the production of GM-CSF, including typical inflammatory mediators/inducers, such as tumor necrosis factor-a, 41 IL-1, 42 and LPS. 42 In addition to GM-CSF, IL-3 enhanced Siglec-F expression (see Supplementary material, Fig.…”

Dexamethasone regulation of the expression of cytokine mRNAs induced by interleukin‐1 in the astrocytoma cell line U373MG

“…Its biological activities include induction of various cytokines. IL-1 has been shown to induce the production of granulocytemacrophage colony-stimulating factor [5][6][7][8][9][10][11][12][13], granulocyte CSF (G-CSF) [9][10][11][12] and B-cell stimulatory factor-2/interleukin-6/interferon-fl2 (IFN-~2)/26-kDa protein [14][15][16][17]. Recently it has also been reported that IL-1 can induce its own synthesis [18][19][20].…”

Tissue distribution of murine hemopoietic growth factor mRNA production

“…The proliferation and differentiation of hemopoietic progenitor cells and the biological activities of mature cells are thought to be controlled at least in part by a family of hemopoietic growth factors including those referred to as the colony-stimulating factors (CSF) (Metcalf, 1984). While a wide variety of murine cell types have been shown to produce specific hemopoietic growth factor mRNAs and bioactivities in vitro (Metcalf, 1984;Rennick et al, 1987;Oppenheim et al, 1986;Dexter, 1987;Arai et al, 1986), relatively little is known about the sites of production in vivo. Indeed, for such factors as IL-2 and IL-3 no evidence for production in vivo has yet been reported.…”