Six field studies were completed in Ontario (during 2016 to 2018) to assess the tolerance of adzuki, kidney, small red and navy bean to 2,4-D ester at 528 or 1056 g•ai•ha −1 applied 14, 7 and 1 day before seeding (PP) and 3 days after seeding (PRE). 2,4-D applied PP or PRE caused as much as 4%, 6%, 7% and 8% injury in adzuki, kidney, small red and navy (white) bean, respectively. There was an increase in bean injury as the preplant interval decreased. At 1 WAE, 2,4-D applied at 1056 g•ai•ha −1 14, 7 and 1 day PP and 3 days after seeding caused up to 6%, 10%, 18% and 5% visible bean injury, respectively. The level of injury decreased over time with minimal bean injury (0 to 3%) at 8 WAE. Bean stand counts were similar to the non-treated control with 2,4-D applied at various timings except at 1 day PP when 2,4-D at the 2X rate decreased bean stand 13%. There was up to 23% and 43% decrease in bean dry weight with 2,4-D applied PP at 528 and 1056 g•ai•ha −1 7 and 1 day PP, respectively. Bean height (6 WAE) was not affected by 2,4-D applied at various timings except at 1 day PP when 2,4-D (1056 g•ai•ha −1) decreased bean height 10%. Additionally, there was no effect of 2,4-D treatments on bean maturity or yield. Based on these results, the safest times to apply 2,4-D are 14 days before seeding or PRE. Injury was higher when 2,4-D was applied 7 and 1 day PP. Injury was lower in adzuki bean compared to kidney, small red or navy bean.