Control of glycolysis in cerebral cortex slices

Rolleston, Francis S.; Newsholme, Eric A.

doi:10.1042/bj1040524

Cited by 132 publications

(46 citation statements)

References 29 publications

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“…Indeed, earlier suggestions (58,59) have been made that an association of G3PD and PGK with membranes may denote a separate ATP-generating pool for membrane-bound ATP-dependent processes and that a G3PD and aldolase complex may be involved as initiators of energy production (35). Of importance is the finding that G3PD is a key enzyme involved in control of glycolysis in cerebral cortex slices (60). Recently, Han et al (61) found that the triadic junction of skeletal muscle contains a compartmentalized reaction sequence of four glycolytic enzymes active in the synthesis of ATP.…”

Section: Discussionmentioning

confidence: 99%

The synthesis of ATP by glycolytic enzymes in the postsynaptic density and the effect of endogenously generated nitric oxide

Aoki

Elste

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

109

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following correction should be noted. Due to an editorial change at PNAS, the meaning of the last sentence on page 14046 was altered. The sentence originally read as follows: On the other hand, this structure does not reproduce the pharmacological properties of either P or Q channel exactly, as the ID 50 to sFTX and -Aga IVA for P-type channels is lower than for the ␣1A, ␣2␦, ␤Ib channels in HEK cells.Neurobiology. In the article "The synthesis of ATP by glycolytic enzymes in the postsynaptic density and the effect of endogenously generated nitric oxide" Kuo Wu, Chiye Aoki, Alice Elste, Adrienne A. Rogalski-Wilk, and Philip Siekevitz, which appeared in number 24, November 25, 1997, of Proc. Natl. Acad. Sci. USA (94,(13273)(13274)(13275)(13276)(13277)(13278), the quality of the reproduction of Fig. 2A was poor. The figure and its legend are shown below:Biochemistry. In the article "KSR stimulates Raf-1 activity in a kinase-independent manner" by Neil R. Michaud, Marc Therrien, Angela Cacace, Lisa C. Edsall, Sarah Spiegel, Gerald M. Rubin, and Deborah K. Morrison, which appeared in number 24, November 25, 1997, of Proc. Natl. Acad. Sci. USA (94,(12792)(12793)(12794)(12795)(12796), the following correction should be noted.Due to a printer's error, background was incorrectly added to (50 g) and 100 g each of the other fractions, in 100 l final volume, including whole homogenate (H), synaptosomes (Syn), synaptic plasma membranes (SPM), and crude synaptic vesicles (CSV), were incubated at 37°C for 15 min. NAD incorporation was performed in the absence (Ϫ) or presence (ϩ) of SNP as exogenous source of NO. The mixtures were subjected to SDS͞PAGE and then autoradiography. (B) Western blot analysis of the G3PD in the subcellular fractions. To confirm that the radioactive protein in the subcellular fractions was indeed G3PD, Western blot analysis was performed by using specific anti-G3PD antibodies as described.

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Section: Discussionmentioning

confidence: 99%

The synthesis of ATP by glycolytic enzymes in the postsynaptic density and the effect of endogenously generated nitric oxide

Aoki

Elste

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

109

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“…Although one naturally must be circumspect in this type of estimation, it does signify possible biological relevance. Assuming that DNA damage is proportional to Glc-6-P concentration (4) and that the intracellular Glc-6-P concentration is 0.25 mM (19), then a 4.2 logarithm inactivation (25 days for 200 mM Glc-6-P, Fig. 1) for the amp gene indicates that the human genome on the average would suffer 2.7 Glc-6-P "hits" per day.…”

Section: Discussionmentioning

confidence: 99%

Modification of DNA by glucose 6-phosphate induces DNA rearrangements in an Escherichia coli plasmid.

Bucala¹,

Model²,

Russel³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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Reducing sugars such as glucose or glucose 6-phosphate (Glc-6-P) have been shown previously to modify the amino groups of nucleotides and single-stranded DNA. We have examined the mutagenic effect of Glc-6-P-induced lesions in the double-stranded DNA plasmid pBR322. Seventeen mutants of the Amp' Tet8 phenotype were isolated from plasmid preparations whose transforming capacity had been decreased by incubation with Glc-6-P. A number of the mutant plasmids were found to have undergone gross DNA alterations, including insertions and deletions, as well as the development of multiple species originating from a single cell. The ability of an endogenous reducing sugar to induce extensive DNA rearrangements suggests that these lesions may be significant contributors to cellular mutation. A recent investigation has demonstrated that the amino groups of nucleic acids can serve as substrates for modification by reducing sugars, leading to spectral changes similar to those described for the nonenzymatic browning ofproteins (4). Glucose or glucose 6-phosphate (Glc-6-P) can modify a single-stranded viral genome, reducing its ability to transfect Escherichia coli at a rate that is directly proportional to incubation time and sugar concentration. The inhibition of transfection also has been shown to occur at a much faster rate than DNA strand-scission (4,5).In the present investigation, we have examined the mutagenicity of Glc-6-P-induced lesions in the double-stranded DNA plasmid pBR322. Mutant plasmids, selected for the Amp' Tet' phenotype, were isolated from plasmid preparations that had been incubated with Glc-6-P and then transformed into an E. coli host. Several of the mutant plasmids were found to have undergone extensive DNA alterations, including insertions and deletions, and the development of multiple species originating from a single transformed cell. MATERIALS AND METHODSPlasmid Transformation. Plasmid pBR322 was purified by cesium chloride density centrifugation (6). One microgram of DNA was incubated at 37TC under sterile conditions in the dark with Glc-6-P (Calbiochem) in 0.2 ml of buffer containing 50 mM Hepes (pH 8.0) and 1.0 mM EDTA. At intervals, 5-to 20-1.l aliquots were transformed (7) into CaCl2-treated E. coli strain K38 (0.3 ml). One milliliter of L broth was then added to each transformation and the cells were incubated for 1 hr at 370C to allow for the expression of antibiotic resistance. Cells were plated onto ampicillin-supplemented TY plates (50 pug/ml) and colonies were counted after an overnight incubation at 370C.Tetracycline sensitivity was tested by stabbing individual ampicillin-resistant colonies into tetracycline-supplemented TY plates (10 ,ug/ml). Colonies also were tested by positive selection on medium containing fusaric acid (8).Southern Hybridization. An inoculum from a single colony was placed into 40 ml of tryptone broth and harvested after bacterial growth reached an OD660 of 1-1.4. Total E. coli DNA was prepared by the method ofSmith (9), and DNA content was determined by a...

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“…An additional contributing factor explaining the elevation of PEP may also include the possibility that enzymatic regulation was operating on pyruvate kinase. which potentially may be one ratelimiting glycolytic enzyme in cerebral (25,33) and other (6) tissues.…”

Section: Cerebral Amino Acid a N D Related Intermediatesmentioning

confidence: 99%

Fetal and Neonatal Cerebral Metabolism Following Maternal Canine Starvation

MIETTINEN

Rolin

et al. 1981

Pediatric Research

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Pediatr. Res. 15: 859-865 (1981) pmoles/g) hr, whereas UDP-glucose concentrations were significantly elevated in these pups during fetal, 3, 9, and 24 hr of age. Phosphoenolpyruvate was higher after maternal starvation in the fetus and at 6 and 9 hr, whereas cerebral pyruvate concentrations were elevated at 3, 6, and 9 hr of age. The elevation of pyruvate with no alteration of lactate concentration resulted in an elevated cytoplasmic NAD/NADH ratio at 3 hr of age (1381 f 194 versus 792 2 198). Cerebral a-ketoglutarate and calculated oxaloacetate concentrations were elevated throughout the day after maternal starvation whereas malate concentrations were depressed at 3 and 9 hr of age. Cerebral energy charge was unaffected, whereas the calculated energy reserve was lower a t 3.6, and 24 hours. Cerebral amino acids demonstrated elevated aspartate concentrations at 3 and 6 hr. Cerebral glutamine concentrations were lower during fetal stage (7.86 2 0.52 versus 10.01 0.41 pmoles/g) and 3, 6, and 9 hr of life. SpeculationThese data suggest that cerebral glycogen synthesis was diminished and that the oxidation of cerebral amino acids, in particular glutamine, may contribute to maintain cerebral energy production during the relative fasting neonatal hypoglycemia which develops in pups after maternal starvations.

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Control of glycolysis in cerebral cortex slices

Cited by 132 publications

References 29 publications

The synthesis of ATP by glycolytic enzymes in the postsynaptic density and the effect of endogenously generated nitric oxide

The synthesis of ATP by glycolytic enzymes in the postsynaptic density and the effect of endogenously generated nitric oxide

Modification of DNA by glucose 6-phosphate induces DNA rearrangements in an Escherichia coli plasmid.

Fetal and Neonatal Cerebral Metabolism Following Maternal Canine Starvation

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