Control of Fecal Peritoneal Infection in Mice by Colony-Stimulating Factors

Barsig, Johannes; Bundschuh, Daniela S.; Hartung, Thomas; Bauhofer, A.; Sauer, Achim; Wendel, Albrecht

doi:10.1093/infdis/174.4.790

Cited by 46 publications

(40 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We now firmly establish that this early reaction significantly contributes to an effective antibacterial response. Of note, although neutrophils are crucial in host defense against a wide array of pathogens, their contribution is not the same for all infections or even for different routes of infection with the same pathogen (3,4,8,9,11,24,25,33,36,42). Neutrophils were found to impact the efficacy of therapeutic interventions (i.e., granulocyte colony-stimulating factor or IFN-␥ given together with appropriate antibiotics) after i.p.…”

Section: Discussionmentioning

confidence: 99%

Neutrophils Are Essential for Rapid Clearance ofEnterococcus faeciumin Mice

Leendertse

Willems²,

Giebelen

et al. 2009

Infect Immun

View full text Add to dashboard Cite

A progressive increase in infections with multiresistant Enterococcus faecium has been reported, especially in cancer patients and neutropenic patients. Despite its increasing importance as a nosocomial pathogen, knowledge of the pathogenesis of E. faecium infections is highly limited. In this study, we investigated the role of neutrophils during peritonitis with subsequent bacteremia caused by E. faecium. Therefore, we depleted neutrophils by intraperitoneal injections of monoclonal antibody RB6-8C5. Mice were followed for 5 days, and the enterococcal outgrowth and inflammatory response were compared between neutropenic mice and immunoglobulin G-injected control mice. Neutropenic mice demonstrated a severe delay in enterococcal clearance from all cultured organs (peritoneal fluid, blood, and lung and liver tissue). In particular, neutropenic mice remained bacteremic for up to 3 days, whereas all nonneutropenic mice had cleared the bacteria from circulation by 2 days. Furthermore, neutropenic mice displayed elevated peritoneal cytokine and chemokine levels 1 day after the infection and attracted fewer macrophages into the peritoneal cavity. In the circulation, a prolonged elevation of tumor necrosis factor alpha, interleukin-6, and the acute-phase proteins serum amyloid A and complement 3 were measured in neutropenic mice. In conclusion, attraction of neutrophils to the primary site of E. faecium infection is important for a rapid clearance of this bacterium, thereby attenuating a systemic inflammatory response.

show abstract

Section: Discussionmentioning

confidence: 99%

Neutrophils Are Essential for Rapid Clearance ofEnterococcus faeciumin Mice

Leendertse

Willems²,

Giebelen

et al. 2009

Infect Immun

View full text Add to dashboard Cite

show abstract

“…This protocol was specifically shown by Seiler et al to spare cells other than neutrophilic granulocytes (59). Alternatively, 100 l of an anti-granulocyte-colony-stimulating factor (anti-GCSF) sheep antiserum was injected intravenously 5 and 3 days before infection, as described previously (6). As a control, the same amount of rat or sheep immunoglobulin G (IgG) was administered.…”

Section: Methodsmentioning

confidence: 99%

Genetically Determined Susceptibility to Tuberculosis in Mice Causally Involves Accelerated and Enhanced Recruitment of Granulocytes

Hoffmann²,

et al. 2006

View full text Add to dashboard Cite

Classical twin studies and recent linkage analyses of African populations have revealed a potential involvement of host genetic factors in susceptibility or resistance to Mycobacterium tuberculosis infection. In order to identify the candidate genes involved and test their causal implication, we capitalized on the mouse model of tuberculosis, since inbred mouse strains also differ substantially in their susceptibility to infection. Two susceptible and two resistant mouse strains were aerogenically infected with 1,000 CFU of M. tuberculosis, and the regulation of gene expression was examined by Affymetrix GeneChip U74A array with total lung RNA 2 and 4 weeks postinfection. Four weeks after infection, 96 genes, many of which are involved in inflammatory cell recruitment and activation, were regulated in common. One hundred seven genes were differentially regulated in susceptible mouse strains, whereas 43 genes were differentially expressed only in resistant mice. Data mining revealed a bias towards the expression of genes involved in granulocyte pathophysiology in susceptible mice, such as an upregulation of those for the neutrophil chemoattractant LIX (CXCL5), interleukin 17 receptor, phosphoinositide kinase 3 delta, or gamma interferon-inducible protein 10. Following M. tuberculosis challenge in both airways or peritoneum, granulocytes were recruited significantly faster and at higher numbers in susceptible than in resistant mice. When granulocytes were efficiently depleted by either of two regimens at the onset of infection, only susceptible mice survived aerosol challenge with M. tuberculosis significantly longer than control mice. We conclude that initially enhanced recruitment of granulocytes contributes to susceptibility to tuberculosis.

show abstract

“…After 24 h of incubation at 37°C and 5% CO 2 , the supernatants were stored at Ϫ70°C until cytokine measurement. To study the involvement of mediators in tolerance induction, neutralizing antibodies (anti-IL-10 [Endogen, Eching, Germany], pan-specific anti-transforming growth factor ␤ [anti-TGF-␤; R&D Systems, Wiesbaden, Germany], and polyclonal anti-mu-granulocyte colony-stimulating factor [G-CSF]-sheep immunoglobulin G raised in our laboratory [2]) or IL-10 (a kind gift from S. Narula, Schering Plough, Kenilworth, N.J.) was added for the first 24-h incubation period.…”

Section: Methodsmentioning

confidence: 99%

Borrelia burgdorferi-Induced Tolerance as a Model of Persistence via Immunosuppression

et al. 2003

View full text Add to dashboard Cite

If left untreated, infection withCross-tolerance towards all tested stimuli was induced. Furthermore, using primary bone marrow cells from TLR2-deficient mice and from mice with a nonfunctional TLR4 (strain C3H/HeJ), we demonstrated that the TLR2 was required for tolerance induction by Borrelia, and using neutralizing antibodies, we identified interleukin-10 as the key mediator involved. Although peripheral blood mononuclear cells tolerized by Borrelia exhibited reduced TLR2 and TLR4 mRNA levels, the expression of the respective proteins on monocytes was not decreased, ruling out the possibility that tolerance to Borrelia is attributed to a reduced TLR2 expression. In summary, we characterized tolerance induced by B. burgdorferi, describing a model of desensitization which might mirror the immunosuppression recently attributed to the persistence of Borrelia in immunocompetent hosts.

show abstract

Control of Fecal Peritoneal Infection in Mice by Colony-Stimulating Factors

Cited by 46 publications

References 47 publications

Neutrophils Are Essential for Rapid Clearance ofEnterococcus faeciumin Mice

Neutrophils Are Essential for Rapid Clearance ofEnterococcus faeciumin Mice

Genetically Determined Susceptibility to Tuberculosis in Mice Causally Involves Accelerated and Enhanced Recruitment of Granulocytes

Borrelia burgdorferi-Induced Tolerance as a Model of Persistence via Immunosuppression

Contact Info

Product

Resources

About