Control of cytokine gene expression by PG101, a water-soluble extract prepared from Lentinus lepideus

Choi, Jeong June; Jin, Myung; Lee, Jong Kyu; Lee, Wi Young; Park, Yong-il; Han, Yong; Kim, Sun‐Young

doi:10.1016/j.bbrc.2005.11.109

Cited by 15 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sugar residues Chemical shifts, ı (ppm) 13 C NMR spectra of WPLE-N-2 and WPLE-N-3 showed several signals in the anomeric region probably due to the presence of ␣-d-linked glycopyranosyl, galactopyranosyl and mannopyranosyl in these polysaccharides. The major resonance in the anomeric region occurs at 101.95-96.85 ppm rather than at 90 ppm, indicating that C-1 is linked [32].…”

Section: Fractionmentioning

confidence: 99%

“…There are reports about the isolation, structural analysis and bioactivities of the polysaccharides from L. edodes. For instance, Lentinan, a (1 → 3)-␤-d-glucan, is the most important polysaccharide isolated from L. edodes, because of its immunomodulatory and antitumor effects [7][8][9]; fucomannogalactan [1], mannoglucan [3], galactoglucomannan [10], ␣-d-glucan [11] and ␤-d-glucan [6,12,13] have been isolated from its basidiocarp, mycelium and culture Gibco (Grand Island, NY, USA). 3-(4,5-Dimethylthyiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma (St. Louis, MO, USA).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Purification and in vitro anti-proliferative effect of novel neutral polysaccharides from Lentinus edodes

Jeff

Yuan

Sun

et al. 2013

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Section: Fractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Purification and in vitro anti-proliferative effect of novel neutral polysaccharides from Lentinus edodes

Jeff

Yuan

Sun

et al. 2013

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

“…The extract of the mycelium of T. matsutake, a biopharmaceutical polysaccharide component, has a wide range of medicinal effects, including reduced oxidation, inhibition of inflammation and anticancer properties (16,19,27,28). However, the anti-aging effects of the mycelium of T. matsutake in human fibroblasts are not yet well known, and to the best of our knowledge, its effects on MMP-1 and TIMP-1 expression have not been reported to date.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, β-glucan is presently available as an ingredient in cosmetic formulations and is found in extracts from mushroom, yeasts, various fungi, cereals and seaweed (18). The extract of the mycelium of T. matsutake also has strongly bioactive properties, exerting antioxidant, cholesterol-lowering, heart disease prevention, and antitumoral, anti-aging, anti-wrinkle and anti-acne effects (16,19,20). The most promising treatments in skin aging include herbal extracts, several vitamins and antioxidant food supplements, which have been widely accepted to enhance collagen and elastin production in the ECM and reduce the levels of dermal enzymes, as MMPs and elastase, thereby restoring skin elasticity and delaying the process of wrinkle formation.…”

Section: Extract Of the Mycelium Of T Matsutake Inhibits Elastase Acmentioning

confidence: 99%

Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts

Kim

Song

et al. 2014

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Abstract. Skin aging is induced through complex biological processes in human skin caused by proteolysis of collagen and elastin, two structural proteins of the dermal extracellular matrix (ECM). Collagen and elastin degradation can induce the expression of matrix metalloproteinases (MMPs), as well as that of a family of zinc-dependent endopeptidases that play critical roles in skin aging. Moreover, elastase is a metalloproteinase which acts on the degradation of elastin in skin aging, and is also involved in the inhibition or the repair of wrinkle formation. Extract of the mycelium of Tricholoma matsutake (T. matsutake), or pine mushroom, is widely distributed in Asian countries. The extract is from the natural biomaterial of the mushroom which is rich in polysaccharides, including β-glucan. This extract has shown potent bioactive antioxidant, immunomodulatory and antitumoral properties. In the present study, we investigated whether the extract of the mycelium of T. matsutake has effects on elastase activity, as well as on the expression of tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) and MMP-1 under basal conditions. Our results revealed that the extract of the mycelium of T. matsutake significantly decreased elastase activity in a dose-dependent manner and reduced the levels of MMP-1 and MMP-3. On the other hand, the expression of TIMP-1 and tropoelastin was increased in fibroblasts treated with the extract of the mycelium of T. matsutake. However, collagent expression was not affected. In addition, our results demonstrated that the extract of the mycelium of T. matsutake inhibited the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced MMP-1 expression and suppressed TPA-induced p38 activity. Therefore, the inhibitory effects of the extract of the mycelium of T. matsutake on MMP-1 induction are mediated by the inhibition of p38 in human fibroblasts. Our data suggest that the extract of the mycelium of T. matsutake may prove to be an effective biomaterial for anti-wrinkle treatment, as it can obstruct the degradation of the dermal ECM.

show abstract

“…Traditionally, patients suffering from malignant diseases would maintain their anticancer defence response with the help of β-glucans (or polysaccharide fractions) obtained from medicinal mushrooms of Korea, China, and Japan. Various polysaccharide fractions such as PG101 (a water-soluble extract that consists of protein-bound polysaccharides), grifolan (an antitumor β-glucan), and PL (an acidic polysaccharide) have been identified as immunostimulating components (Choi et al, 2006) that function by activating the macrophages (Kodama et al, 2003;Jeong et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Mitogen activated protein kinases are prime signalling enzymes in nitric oxide production induced by soluble β-glucan from Sparassis crispa

Lee

Byeon

et al. 2010

Arch. Pharm. Res.

View full text Add to dashboard Cite

Sparassis crispa (SC) is an edible mushroom that harbours β-glucans reported to possess immunostimulatory and anticancer properties. The role of SC in regulating the functional activation of macrophages is yet to be fully elucidated. The objective of this study was to investigate the molecular mechanism underlying the immune-stimulatory function of Sparassis crispa soluble β-glucan (Sc-SG) on macrophages. According to this study, Sc-SG was able to stimulate nitric oxide (NO) production as well as enhance the expression of inducible NO synthase (iNOS) from macrophage-like RAW264.7 cells. NO production was strongly suppressed by mitogen-activated protein kinase (MAPK) inhibitors such as U0126, extracellular signal-regulated kinase, SB203580, a p38 inhibitor, and SP600125, a c-Jun N-terminal kinase inhibitor. Thus, indicating that Sc-SG-induced NO release is possibly mediated by MAPK. Sc-SG induced phosphorylation of extracellular signal-regulated kinase, p38, and JNK in a time-dependent manner. Moreover, Sc-SG triggered the phosphorylation and translocation of c-Jun and c-Fos, components of the transcription factor AP-1, activated by MAPK. The results of this study suggest that MAPK may be a major signaling enzyme that regulates the Sc-SG-mediated NO production in macrophages.

show abstract

Control of cytokine gene expression by PG101, a water-soluble extract prepared from Lentinus lepideus

Cited by 15 publications

References 26 publications

Purification and in vitro anti-proliferative effect of novel neutral polysaccharides from Lentinus edodes

Purification and in vitro anti-proliferative effect of novel neutral polysaccharides from Lentinus edodes

Extract of the mycelium of T. matsutake inhibits elastase activity and TPA-induced MMP-1 expression in human fibroblasts

Mitogen activated protein kinases are prime signalling enzymes in nitric oxide production induced by soluble β-glucan from Sparassis crispa

Contact Info

Product

Resources

About