Control of Crown Gall in Swiss Apple Nurseries

Grimm, Rainer; Vogelsanger, J.

doi:10.1007/978-94-009-3555-6_13

Cited by 2 publications

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“…The bacterium is also widely used as a genetic tool for delivering foreign DNA into plant cells to generate transgenic plants (15). A. tumefaciens is commonly exposed to high levels of Cu ions as a consequence of the use of Cu-based bactericides containing, viz., Cu(OH) 2 , CuSO 4 , and Cu oxychloride for plant pathogen management (23,25). The mechanism by which the bacterium deals with Cu stress is unknown.…”

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Functional and Expression Analyses of the cop Operon, Required for Copper Resistance in Agrobacterium tumefaciens

et al. 2009

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The copper resistance determinant copARZ, which encodes a CPx-type copper ATPase efflux protein, a transcriptional regulator, and a putative intracellular copper chaperone, was functionally characterized for the phytopathogenic bacterium Agrobacterium tumefaciens. These genes are transcribed as an operon, and their expression is induced in response to increasing copper and silver ion concentrations in a copR-dependent fashion. Analysis of the copARZ promoter revealed a putative CopR binding box located within the spacer of the ؊35 and ؊10 promoter motifs. In vitro, purified CopR could specifically bind to the box. The inactivation of the copARZ operon or copZ reduces the level of resistance to copper but not to other metal ions. Also, the copARZ operon mutant shows increased sensitivity to the superoxide generators menadione and plumbagin. In addition, the loss of functional copZ does not affect the ability of copper ions to induce the copARZ promoter, indicating that CopZ is not involved in the copper-sensing mechanism of CopR. Altogether, the results demonstrate a crucial role for the copARZ operon as a component of the copper resistance machinery in A. tumefaciens.

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Functional and Expression Analyses of the cop Operon, Required for Copper Resistance in Agrobacterium tumefaciens

et al. 2009

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“…1). Uber wirtschaftliche Schaden wurde an Steinfriichten in Spanien, Australien und den USA berichtet (Kerster & de Ley, 1984); (Lopez et al, 1987) sowie in Baumschulen mit vorwiegend Apfelkulturen in der Schweiz und Spanien (Grimm, 1987); (Lopez et al, 1987). In den norddeutschen Baumschulen bilden Vermehrung und Anzucht von Pflanzen aus der Familie der Rosaceae einen Schwerpunkt.…”

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Vergleichende untersuchungen zum nachweis vonagrobacterium tumefaciensmittels polymerase kettenreaktion, serologischer methoden, sds‐page und selektiven nährmedien

Sadowska-Rybak

Büttner

Landsiedel

1995

Archives Of Phytopathology And Plant Protection

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Control of Crown Gall in Swiss Apple Nurseries

Cited by 2 publications

References 2 publications

Functional and Expression Analyses of the cop Operon, Required for Copper Resistance in Agrobacterium tumefaciens

Functional and Expression Analyses of the cop Operon, Required for Copper Resistance in Agrobacterium tumefaciens

Vergleichende untersuchungen zum nachweis vonagrobacterium tumefaciensmittels polymerase kettenreaktion, serologischer methoden, sds‐page und selektiven nährmedien

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