Control of copper homeostasis in Escherichia coli by a P-type ATPase, CopA, and a MerR-like transcriptional activator, CopR

Petersen, Claus Leth; Møller, Lisbeth Birk

doi:10.1016/s0378-1119(00)00509-6

Cited by 100 publications

(97 citation statements)

References 25 publications

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“…2a). The Salmonella copA transcription start site differs in one base from the one determined previously in E. coli by Outten et al, (2000), Petersen & Moller (2000) and Stoyanov et al (2001).…”

Section: Expression Of Copa In Salmonella Is Directly Controlled By Cuercontrasting

confidence: 58%

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Dissecting the Salmonella response to copper

et al. 2007

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Section: Expression Of Copa In Salmonella Is Directly Controlled By Cuercontrasting

confidence: 58%

“…CueR directly stimulates the transcription of copA and cueO, coding for a P-type ATPase and a multicopper oxidase, respectively. CopA is predicted to translocate Cu(I) from the cytoplasm to the periplasmic space, where it is converted to the less toxic form Cu(II) by CueO Petersen & Moller, 2000;.…”

Section: Introductionmentioning

confidence: 99%

Dissecting the Salmonella response to copper

et al. 2007

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“…These observations could suggest that the pig cluster may be under CueR-mediated control and/or that one or more of the Pig enzymes requires copper, or a related metal, as a cofactor. The CueR/CopA system is involved in copper efflux (Changela et al, 2003;Petersen & Møller, 2000;Stoyanov et al, 2001). It is therefore intriguing that prodigiosin has been shown to effect Cu-dependent DNA cleavage (Melvin et al, 2001;Park et al, 2003) and so it is conceivable that intracellular copper availability must be regulated in concert with prodigiosin biosynthesis to avoid DNA damage.…”

Section: Relative Genomic Contexts Of the Pig Clustersmentioning

confidence: 99%

The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation

et al. 2004

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The prodigiosin biosynthesis gene cluster (pig cluster) from two strains of Serratia (S. marcescens ATCC 274 and Serratia sp. ATCC 39006) has been cloned, sequenced and expressed in heterologous hosts. Sequence analysis of the respective pig clusters revealed 14 ORFs in S. marcescens ATCC 274 and 15 ORFs in Serratia sp. ATCC 39006. In each Serratia species, predicted gene products showed similarity to polyketide synthases (PKSs), non-ribosomal peptide synthases (NRPSs) and the Red proteins of Streptomyces coelicolor A3(2). Comparisons between the two Serratia pig clusters and the red cluster from Str. coelicolor A3(2) revealed some important differences. A modified scheme for the biosynthesis of prodigiosin, based on the pathway recently suggested for the synthesis of undecylprodigiosin, is proposed. The distribution of the pig cluster within several Serratia sp. isolates is demonstrated and the presence of cryptic clusters in some strains shown. The pig cluster of Serratia marcescens ATCC 274 is flanked by cueR and copA homologues and this configuration is demonstrated in several S. marcescens strains, whilst these genes are contiguous in strains lacking the pig cluster.

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“…It shows similarity to copper pumps from several prokaryotic and eukaryotic sources, including 31 and 29% identity to the human Wilson's and Menkes' proteins, respectively. The transcription of copA is regulated solely by CueR (GeneBank TM AF318185), a copper-and silver-responsive (7,8,10) member of the MerR family of transcriptional activators (Fig. 1).…”

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confidence: 99%