Control of citrus blue mold by the antagonist yeast Pichia guilliermondii Z1: Compatibility with commercial fruit waxes and putative mechanisms of action

Lahlali, Rachid; Hamadi, Younes; Drider, R.; Misson, C; Guilli, Mohammed El; Jijakli, M. Haïssam

doi:10.1016/j.foodcont.2014.04.014

Cited by 29 publications

(13 citation statements)

References 45 publications

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“…Currently, the blue mold is primarily controlled by the synthetic fungicide applications, such as thiabendazole and imazalil (Sun et al, 2013). However, problems related to the development of pathogen resistance to many site-specific fungicides and to the potentially harmful effects on human safety and environment have stimulated researches related to alternative methods for disease control in postharvest (Lahlali et al, 2014;Palou et al, 2002;Zhou et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum

Cunha

Ferraz

Wehr

et al. 2018

International Journal of Food Microbiology

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Penicillium italicum (Blue mold) is a major postharvest disease of citrus. An alternative to controlling the disease is through the use of yeasts. The purpose of the present study was to screen effective yeast antagonists against P. italicum, isolated from soil, leaves, flowers, and citrus fruits, to assess the action mechanisms of the yeast isolates that were demonstrated to be effective for biocontrol, and to identify the most effective yeast isolates for the biocontrol of blue mold. The in vitro assays showed that six yeast strains inhibited up to 90% of the pathogen's mycelial growth. In vivo assays, evaluating the incidence of blue mold on sweet oranges, the strains ACBL-04, ACBL-05, ACBL-10 and ACBL-11 were effective, demonstrating the potential for the blue mold control when preventively applied, whereas the ACBL-08 strain showed a high potential to preventive and curative applications. Additional studies on the modes of action of these yeast strains showed that most of the evaluated yeast strains did not produce antifungal substances, in sufficient quantities to inhibit the pathogen growth. Competition for nutrients was not a biocontrol strategy used by the yeast strains. The 'killer' activity might be the main action mechanism involved in P. italicum biocontrol. This study indicated that the multiple modes of action against the pathogen presented by yeasts may explain why these strains provided P. italicum control under in vitro and in vivo conditions. However, further studies in future might be able to elucidate the 'killer' activity and its interaction with pathogen cells and the bioproduct production using Candida stellimalicola strains for control postharvest diseases.

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Section: Introductionmentioning

confidence: 99%

Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum

Cunha

Ferraz

Wehr

et al. 2018

International Journal of Food Microbiology

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“…Of eight yeasts isolated for their antagonistic activity against the pathogen, two isolates of Pichia kluyveri (M45) and Metschnikowia pulcherrima (M54) had the most effective. Previously reports showed a number of effective yeasts isolate for the biological control of Penicillium italicum on citrus (Arras et al, 2002;Lahlali et al, 2014). The main mechanism of this biological control is based on induction of host defences (Janisiewicz and Korsten, 2002;Massart and Jijakli, 2007).…”

Section: Discussionmentioning

confidence: 99%

Biochemical Changes in Orange Fruit Due to Plant – <i>Penicillium italicum</i> – Antagonism Interactions

GHASEMI

Etebarian

SAHEBANI

et al. 2015

Not Bot Hort Agrobot Cluj

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One of the most important orange fruit diseases is blue mold which cause by Penicillium italicum that is responsible for important economic losses. This study investigated biochemical changes in exo-mesocarp layers of orange fruits related to hostpathogen-yeast interactions. Initial result showed that among eight strains isolated, the most effective antagonist were belonged to two species of Pichia kluyveri (M45) and Rhodotorula mucilaginosa (M61). These isolates were selected for biochemical evaluation. In order to assessment of biochemical changes, the orange fruits were inoculated with 40 µl of yeast cell suspension and after 24 h, the wounds were inoculated with 20 µl of conidial suspension of P. italicum. The analysis of variance showed that all of the measured biochemical characterises were significant in both layers by treatments (yeast isolates; pathogen; yeast isolates + pathogen and control) (P ≤ 0.01), including POD, CAT and β-1, 3-glucanase activities and total phenolic compounds. Also result showed that when the yeast isolates (M45 or M54) were inoculated into wounds with the pathogen, it stimulated the orange to increase produce of total phenol and enzymes activity (POD, CAT and β-1, 3-glucanase) and these changes were related to incubation time. The result showed that understanding biochemical mechanism derived from plant-pathogen-antagonist interactions is essential for investigating the dynamics of infectious processes.

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“…In addition to UV-C treatment, biological control utilizing antagonistic yeasts has been examined as a potential alternative to chemical fungicides in managing postharvest decay (Wilson and Wisniewski 1989;Wisniewski and Wilson 1992;Droby et al 2009;Liu et al 2013). Many yeasts in the genus Pichia, including Pichia membranaefaciens, Pichia anomala, Pichia guilliermondii, and Pichia caribbica, have been reported as effective biocontrol agents for the control of postharvest diseases of apple, citrus, banana, and kiwifruit (Wisniewski et al 1991;Lassois et al 2008;Lahlali et al 2014;Li et al 2014;.…”

Section: Introductionmentioning

confidence: 99%

Combining UV-C treatment with biocontrol yeast to control postharvest decay of melon

Huang

Zou

Luo

et al. 2015

Environ Sci Pollut Res

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Significant losses in harvested melon can be directly attributable to decay fungi. In the present study, the use of UV-C treatment combined with biocontrol yeast, Pichia cecembensis, was evaluated for their ability to control postharvest decay of melon fruits after they were artificially inoculated with Fusarium oxysporum and Alternaria alternata. Natural infection of fruit was also assessed. As a stand-alone treatment, UV-C or P. cecembensis significantly reduced Fusarium rot and Alternaria rot, and also the level of natural infection on melon fruit, relative to the untreated control. The combination of UV-C or P. cecembensis, however, provided a superior level of decay control on artificially inoculated and naturally infected fruit, compared to either treatment alone. None of the treatments impaired fruit quality. Integrating the use of UV-C treatment with biocontrol yeast has potential as an effective method to control postharvest decay of melon.

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Control of citrus blue mold by the antagonist yeast Pichia guilliermondii Z1: Compatibility with commercial fruit waxes and putative mechanisms of action

Cited by 29 publications

References 45 publications

Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum

Antifungal activity and action mechanisms of yeasts isolates from citrus against Penicillium italicum

Biochemical Changes in Orange Fruit Due to Plant – <i>Penicillium italicum</i> – Antagonism Interactions

Combining UV-C treatment with biocontrol yeast to control postharvest decay of melon

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