Control of ciliogenesis by FOR20, a novel centrosome and pericentriolar satellite protein

Sedjaï, Fatima; Acquaviva, Claire; Chevrier, Véronique; Chauvin, Jean‐Paul; Coppin, Emilie; Aouane, Aı̈cha; Coulier, François; Tolun, Aslıhan; Pierres, Michel; Birnbaum, Daniel; Rosnet, Olivier

doi:10.1242/jcs.076828

Cited by 8 publications

(28 citation statements)

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“…Immunofluorescence staining showed that FOR20 colocalized with the centrosomal proteins γ-tubulin and centrin-2 (Supplementary information, Figure S1C), which was validated by co-purification of FOR20 and γ-tubulin in the centrosomal fraction by sucrose gradient centrifugation (Supplementary information, Figure S1D). These results are consistent with the previous studies [11,12]. Next, we used vector-based RNAi to deplete endogenous FOR20 in mammalian cells.…”

Section: The Role Of For20 In S-phase Progressionsupporting

confidence: 92%

“…Recently, an evolutionally conserved protein, FOR20 (FOP-related protein of 20 kDa), has been identified; it is associated with centrosomes and may be involved in ciliogenesis [11,12]. Here, we find that FOR20 is essential not only for S-phase progression, but also for recruiting Plk1 to centrosomes.…”

Section: Introductionmentioning

confidence: 62%

“…BrdU incorporation after double-thymidine block synchronization and release further confirmed the important role of FOR20 in S-phase progression (Supplementary information, Figure S2E). To eliminate the potential off-targeting effects, we employed another vectorbased RNAi that targets the previously described region [12] and found that depletion of endogenous FOR20 indeed induced significant defects in S-phase (Supplementary information, Data S1 and Figure S3). The cells transfected with either pBS/U6 or pBS/U6-FOR20 for 96 h were subjected to immunofluorescence staining with anti-FOR20 and γ-tubulin antibodies.…”

Section: The Role Of For20 In S-phase Progressionmentioning

confidence: 99%

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Centrosomal protein FOR20 is essential for S-phase progression by recruiting Plk1 to centrosomes

et al. 2013

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Centrosomes are required for efficient cell cycle progression mainly by orchestrating microtubule dynamics and facilitating G1/S and G2/M transitions. However, the role of centrosomes in S-phase progression is largely unknown. Here, we report that depletion of FOR20 (FOP-related protein of 20 kDa), a conserved centrosomal protein, inhibits S-phase progression and prevents targeting of Plk1 (polo-like kinase 1) to centrosomes, where FOR20 interacts with Plk1. Ablation of Plk1 also significantly induces S-phase defects, which are reversed by ectopic expression of Plk1, even a kinase-dead mutant, but not a mutant that fails to localize to centrosomes. Exogenous expression of centrosome-tethered Plk1, but not wild-type Plk1, overrides FOR20 depletion-induced S-phase defects independently of its kinase activity. Thus, these data indicate that recruitment of Plk1 to centrosomes by FOR20 may act as a signal to license efficient progression of S-phase. This represents a hitherto uncharacterized role of centrosomes in cell cycle regulation.

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Section: The Role Of For20 In S-phase Progressionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 62%

Section: The Role Of For20 In S-phase Progressionmentioning

confidence: 99%

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Centrosomal protein FOR20 is essential for S-phase progression by recruiting Plk1 to centrosomes

et al. 2013

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“…To determine whether the pCCCs are likely to be centriole components, we transiently expressed N-and C-terminally GFPtagged human orthologs of each pCCC in U2OS and HeLa human 4026 Journal of Cell Science 123 (23) cell lines and used antibodies recognizing g-tubulin to highlight centrosomes. To the eight unknown gene products, we added one whose homolog localizes to the base of the cilia of Caenorhabditis elegans (B9D2) and one that has only very recently been characterized in human cells (MOT52, also known as FOR20 or BBC20) (Sedjai et al, 2010). Five of the ten tagged proteins showed either diffuse cytoplasmic GFP or bright foci likely to be inclusion bodies (data not shown).…”

Section: Verification Of Putative Centriole Genesmentioning

confidence: 99%

“…Recently, the human ortholog (FOR20) was reported to localize to pericentriolar satellites and to be involved in ciliary assembly (Sedjai et al, 2010). FOR20 is predicted to have a FOP dimerization domain (Pfam domain PF09398), required for the centrosomal localization of the FOP protein (Mikolajka et al, 2006).…”

Section: Verification Of Putative Centriole Genesmentioning

confidence: 99%

Ancestral centriole and flagella proteins identified by analysis ofNaegleriadifferentiation

Fritz‐Laylin

Cande

2010

Journal of Cell Science

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SummaryNaegleria gruberi is a single-celled eukaryote best known for its remarkable ability to form an entire microtubule cytoskeleton de novo during its metamorphosis from an amoeba into a flagellate, including basal bodies (equivalent to centrioles), flagella and a cytoplasmic microtubule array. Our publicly available full-genome transcriptional analysis, performed at 20-minute intervals throughout Naegleria differentiation, reveals vast transcriptional changes, including the differential expression of genes involved in metabolism, signaling and the stress response. Cluster analysis of the transcriptional profiles of predicted cytoskeletal genes reveals a set of 55 genes enriched in centriole components (induced early) and a set of 82 genes enriched in flagella proteins (induced late). The early set includes genes encoding nearly every known conserved centriole component, as well as eight previously uncharacterized, highly conserved genes. The human orthologs of at least five genes localize to the centrosomes of human cells, one of which (here named Friggin) localizes specifically to mother centrioles.

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Centrosomal protein FOR20 knockout mice display embryonic lethality and left‐right patterning defects

Liu

Gao

et al. 2021

FEBS Letters

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Centrosomal protein FOR20 has been reported to be crucial for essential cellular processes, including ciliogenesis, cell migration, and cell cycle in vertebrates. However, the function of FOR20 during mammalian embryonic development remains unknown. To investigate the in vivo function of the For20 gene in mammals, we generated For20 homozygous knockout mice by gene targeting. Our data reveal that homozygous knockout of For20 results in significant embryonic growth arrest and lethality during gestation, while the heterozygotes show no obvious defects. The absence of For20 leads to impaired left-right patterning of embryos and reduced cilia in the embryonic node. Deletion of For20 also disrupts angiogenesis in yolk sacs and embryos. These results highlight a critical role of For20 in early mammalian embryogenesis.

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Control of ciliogenesis by FOR20, a novel centrosome and pericentriolar satellite protein

Cited by 8 publications

References 0 publications

Centrosomal protein FOR20 is essential for S-phase progression by recruiting Plk1 to centrosomes

Centrosomal protein FOR20 is essential for S-phase progression by recruiting Plk1 to centrosomes

Ancestral centriole and flagella proteins identified by analysis ofNaegleriadifferentiation

Centrosomal protein FOR20 knockout mice display embryonic lethality and left‐right patterning defects

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