Control of cell lineage-specific development and transcription by bHLH-PAS proteins

Crews, Stephen T.

doi:10.1101/gad.12.5.607

Cited by 311 publications

(235 citation statements)

References 111 publications

Supporting

Mentioning

229

Contrasting

Unclassified

Order By: Relevance

“…The aryl hydrocarbon receptor (AHR) is part of the basic helix-loop-helix Per-ARNT-Sim (bHLH-PAS) protein superfamily of environmental sensors, developmental regulators, and coactivators (Crews 1998;Gu et al 2000;Ledent and Vervoort 2001). Some of the most ubiquitous toxic compounds in the environment, including polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated biphenyls (PCBs), and some polycyclic aromatic hydrocarbons (PAHs), elicit biochemical and toxic responses through the AHR signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Merson

Franks

Karchner

et al. 2006

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

The aryl hydrocarbon receptor (AHR) and AHR repressor (AHRR) proteins regulate gene expression in response to some halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons. The Atlantic killifish is a valuable model of the AHR signaling pathway, but antibodies are not available to fully characterize AHR and AHRR proteins. Using bacterially expressed AHRs, we developed specific and sensitive polyclonal antisera against the killifish AHR1, AHR2, and AHRR. In immunoblots, these antibodies recognized full-length killifish AHR and AHRR proteins synthesized in rabbit reticulocyte lysate, proteins expressed in mammalian cells transfected with killifish AHR and AHRR constructs, and AHR proteins in cytosol preparations from killifish tissues. Killifish AHR1 and AHR2 proteins were detected in brain, gill, kidney, heart, liver, and spleen. Antisera specifically precipitated their respective target proteins in immunoprecipitation experiments with in vitro-expressed proteins. Killifish ARNT2 co-precipitated with AHR1 and AHR2. These sensitive, specific, and versatile antibodies will be valuable to researchers investigating AHR signaling and other physiological processes involving AHR and AHRR proteins.

show abstract

Section: Introductionmentioning

confidence: 99%

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Merson

Franks

Karchner

et al. 2006

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

show abstract

“…1). Around the time of gastrulation, the single-minded midline master regulatory gene activates the midline developmental program (Crews, 1998), and soon after 3 MP equivalence groups (MP1, MP3, MP4) of five or six cells/each form (Wheeler et al, 2008). Notch signaling selects one cell from the MP1 group to become an MP1 and the others become midline glia (MG).…”

Section: Introductionmentioning

confidence: 99%

Dual role for Drosophila lethal of scute in CNS midline precursor formation and dopaminergic neuron and motoneuron cell fate

2011

View full text Add to dashboard Cite

SUMMARYDopaminergic neurons play important behavioral roles in locomotion, reward and aggression. The Drosophila H-cell is a dopaminergic neuron that resides at the midline of the ventral nerve cord. Both the H-cell and the glutamatergic H-cell sib are the asymmetric progeny of the MP3 midline precursor cell. H-cell sib cell fate is dependent on Notch signaling, whereas H-cell fate is Notch independent. Genetic analysis of genes that could potentially regulate H-cell fate revealed that the lethal of scute [l(1)sc], tailup and SoxNeuro transcription factor genes act together to control H-cell gene expression. The l(1)sc bHLH gene is required for all H-cell-specific gene transcription, whereas tailup acts in parallel to l(1)sc and controls genes involved in dopamine metabolism. SoxNeuro functions downstream of l(1)sc and controls expression of a peptide neurotransmitter receptor gene. The role of l(1)sc may be more widespread, as a l(1)sc mutant shows reductions in gene expression in non-midline dopaminergic neurons. In addition, l(1)sc mutant embryos possess defects in the formation of MP4-6 midline precursor and the median neuroblast stem cell, revealing a proneural role for l(1)sc in midline cells. The Notch-dependent progeny of MP4-6 are the mVUM motoneurons, and these cells also require l(1)sc for mVUM-specific gene expression. Thus, l(1)sc plays an important regulatory role in both neurogenesis and specifying dopaminergic neuron and motoneuron identities.

show abstract

“…Class B bHLH proteins are postulated to not homodimerize, rather they are believed to heterodimerize with Class A bHLH proteins. Class C bHLH proteins also contain one or more PAS domains (Crews, 1998). This domain allows for dimerization between PAS proteins, non-PAS proteins and the binding of small molecules (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…This domain allows for dimerization between PAS proteins, non-PAS proteins and the binding of small molecules (e.g. dioxin) (Crews, 1998).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic and expression analysis of the basic helix-loop-helix transcription factor gene family: genomic approach to cellular differentiation

2008

View full text Add to dashboard Cite

A phylogenetic analysis of seven different species (human, mouse, rat, worm, fly, yeast, and plant) utilizing all (541) basic helix-loop-helix (bHLH) genes identified, including expressed sequence tags (EST), was performed. A super-tree involving six clades and a structural categorization involving the entire coding sequence was established. A nomenclature was developed based on clade distribution to discuss the functional and ancestral relationships of all the genes. The position/location of specific genes on the phylogenetic tree in relation to known bHLH factors allows for predictions of the potential functions of uncharacterized bHLH factors, including EST's. A genomic analysis using microarrays for four different mouse cell types (i.e. Sertoli, Schwann, thymic, and muscle) was performed and considered all known bHLH family members on the microarray for comparison. Cell-specific groups of bHLH genes helped clarify those bHLH genes potentially involved in cell specific differentiation. This phylogenetic and genomic analysis of the bHLH gene family has revealed unique aspects of the evolution and functional relationships of the different genes in the bHLH gene family.

show abstract

Control of cell lineage-specific development and transcription by bHLH-PAS proteins

Cited by 311 publications

References 111 publications

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Development and characterization of polyclonal antibodies against the aryl hydrocarbon receptor protein family (AHR1, AHR2, and AHR repressor) of Atlantic killifish Fundulus heteroclitus

Dual role for Drosophila lethal of scute in CNS midline precursor formation and dopaminergic neuron and motoneuron cell fate

Phylogenetic and expression analysis of the basic helix-loop-helix transcription factor gene family: genomic approach to cellular differentiation

Contact Info

Product

Resources

About