Control of calcium homeostasis in Schistosoma mansoni

Noël, François; Cunha, Valéria M.N.; Silva, C.L.M; Mendonça-Silva, Dayde Lane

doi:10.1590/s0074-02762001000900012

Cited by 19 publications

(12 citation statements)

References 23 publications

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“…Host entry, miracidium-sporocyst transformation, muscle movement and larval motility, and enzyme regulation all appear to be calcium-dependent processes (Sponholtz and Short, 1976; Knabe et al . 1982; Noel et al . 2001).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis ofSchistosoma mansonilarval development using serial analysis of gene expression (SAGE)

et al. 2009

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SUMMARY Infection of the snail, Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke, Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genome-wide expression analysis of the S. mansoni miracidia and developing sporocyst using Long Serial Analysis of Gene Expression (LongSAGE). Five cDNA libraries were constructed from miracidia and in vitro cultured 6- and 20-day-old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of the B. glabrata embryonic (Bge) cell line. We generated 21 440 SAGE tags and mapped 13 381 to the S. mansoni gene predictions (v4.0e) either by estimating theoretical 3′ UTR lengths or using existing 3′ EST sequence data. Overall, 432 transcripts were found to be differentially expressed amongst all 5 libraries. In total, 172 tags were differentially expressed between miracidia and 6-day conditioned sporocysts and 152 were differentially expressed between miracidia and 6-day unconditioned sporocysts. In addition, 53 and 45 tags, respectively, were differentially expressed in 6-day and 20-day cultured sporocysts, due to the effects of exposure to Bge cell-conditioned medium.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis ofSchistosoma mansonilarval development using serial analysis of gene expression (SAGE)

et al. 2009

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“…Other calcium‐binding proteins were identified in schistosomes. A sarco/endoplasmic reticulum (Ca 2+ –Mg 2+ )‐ATPase (SERCA) of S. mansoni was found to be 140–250‐fold less sensitive to selective inhibitors of mammalian SERCAs 162. A calmodulin163, 164 and a calreticulin165 from S. mansoni have also been cloned, expressed, and characterized.…”

Section: Anthelminthic Therapymentioning

confidence: 99%

Schistosomiasis Chemotherapy

et al. 2013

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After malaria, schistosomiasis (or bilharzia) is the second most prevalent disease in Africa, and is occurring in over 70 countries in tropical and subtropical regions. It is estimated that 600 million people are at risk of infection, 200 million people are infected, and at least 200,000 deaths per year are associated with the disease. All schistosome species are transmitted through contact with fresh water that is infested with free-swimming forms of the parasite, which is known as cercariae and produced by snails. When located in the blood vessels of the host, larval and adult schistosomes digest red cells to acquire amino acids for growth and development. Vaccine candidates have been unsuccessful up to now. Against such devastating parasitic disease, the antischistosomal arsenal is currently limited to a single drug, praziquantel, which has been used for more than 35 years. Because the question of the reduction of the activity of praziquantel was raised recently, it is thus urgent to create new and safe antischistosomal drugs that should be combined with praziquantel to develop efficient bitherapies.

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“…Similarly as in vertebrate species, the control of intracellular calcium is fundamental in S. mansoni cell physiology, particularly in the function of adult muscle, which shows morphological and biochemical similarities with vertebrate smooth muscle (13). Praziquantel, the only currently available drug used for the effective treatment of schistosomiasis, acts by an unknown mechanism, possibly through the modulation of calcium channel ␤-subunit function (14), to cause a rapid influx of calcium resulting in muscle contraction and paralysis.…”

Section: )mentioning

confidence: 99%

Functional Characterization of a P2X Receptor from Schistosoma mansoni

Agboh

Webb

Evans

et al. 2004

Journal of Biological Chemistry

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The cloning and characterization of a P2X receptor (schP2X) from the parasitic blood fluke Schistosoma mansoni provides the first example of a non-vertebrate ATP-gated ion channel. A number of functionally important amino acid residues conserved throughout vertebrate P2X receptors, including 10 extracellular cysteines, aromatic and positively charged residues involved in ATP recognition, and a consensus protein kinase C site in the amino-terminal tail, are also present in schP2X. Overall, the amino acid sequence identity of schP2X with human P2X 1-7 receptors ranges from 25.8 to 36.6%. ATP evoked concentration-dependent currents at schP2X channels expressed in Xenopus oocytes with an EC 50 of 22.1 M. 2,3-O-(4-Benzoylbenzoyl)adenosine 5-triphosphate (Bz-ATP) was a partial agonist (maximum response 75.4 ؎ 4.4% that of ATP) with a higher potency (EC 50 of 3.6 M) than ATP. Suramin and pyridoxal-phosphate-6-azophenyl-2,4-disulfonic acid blocked schP2X responses to 100 M ATP with IC 50 values of 9.6 and 0.5 M, respectively. Ivermectin (10 M) potentiated currents to both ATP and Bz-ATP by ϳ60% with a minimal effect on potency (EC 50 of 18.2 and 1.6 M, respectively). The relative permeability of schP2X expressed in HEK293 cells to various cations was determined under bi-ionic conditions. schP2X has a relatively high calcium permeability (P Ca /P Na ‫؍‬ 3.80 ؎ 0.29) and an estimated minimum pore diameter similar to that of vertebrate P2X receptors. SchP2X provides a useful comparative model for the better understanding of human P2X receptor function and may also provide an alternative drug target for treatment of schistosomiasis.P2X receptors comprise a family of cation-selective ion channels gated by extracellular ATP. Mammalian species possess seven distinct P2X channel subtypes (P2X 1-7 ), each encoded by a separate gene. These subunits assemble as functional homotrimeric or heterotrimeric channels and play an important role in a wide array of physiological processes including neurotransmission, smooth muscle contraction, immune cell function, and platelet aggregation (for a recent review see Ref. 1).This remarkably diverse range of physiological roles for P2X receptors has contributed to the notion that ATP is a "primitive" extracellular signaling molecule (2, 3). Functional evidence suggests that ATP-gated ion channels exist in some lower organisms including Tetrahymena thermophilia (4), leech (5), and Amoeba proteus (6), supporting the view that the development of P2X receptors for ATP occurred relatively early in the evolution of eukaryotic organisms. However, to date, definitive molecular identification of P2X channels is restricted to vertebrate species including fish (7), amphibians (8, 9), birds (10), and mammals (1).With the recent expansion in the range of species for which genomic and EST 1 sequence databases are available, it is now possible to use a bioinformatics approach to screen a wide range of lower organisms for P2X receptor-like proteins. Using such a strategy, we have identified a P2X channel from...

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Control of calcium homeostasis in Schistosoma mansoni

Cited by 19 publications

References 23 publications

Transcriptome analysis ofSchistosoma mansonilarval development using serial analysis of gene expression (SAGE)

Transcriptome analysis ofSchistosoma mansonilarval development using serial analysis of gene expression (SAGE)

Schistosomiasis Chemotherapy

Functional Characterization of a P2X Receptor from Schistosoma mansoni

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