Control of Bovine Vibriosis by Vaccination

Bl, Clark

doi:10.1111/j.1751-0813.1967.tb08869.x

Cited by 4 publications

(4 citation statements)

References 4 publications

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“…However, as stated previously (Clark 1967), the swellings are easy to remove at slaughter from either the skin or Airslralian Veterinary Journal, Vol. 48, July, 1972 the underlying muscle and are of little economic importance.…”

Section: Discussionmentioning

confidence: 79%

“…The methods used to prepare the cells for vacine SM followed in general those of Clark et al (1967) except that the cells were harvested after growing for four days on the surface of 2.5% nutrient agar enriched with 7.5% defibrinated ox blcod in an atmosphere of 5% oxygen, 5% carbon dioxide and 90% nitrogen, and were adjusted to a density equal to 11 times that of Wellcome* opacity tube 10. The cell suspension was killed by addition of phenol to a final concentration of 1% (w/v).…”

Section: Preparation Of Vaccinesmentioning

confidence: 99%

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IMMUNISATION AGAINST BOVINE VIBRIOSIS: 1. Comparison of the Protective Properties of Bacterins Prepared by Two Methods

1972

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Section: Discussionmentioning

confidence: 79%

Section: Preparation Of Vaccinesmentioning

confidence: 99%

IMMUNISATION AGAINST BOVINE VIBRIOSIS: 1. Comparison of the Protective Properties of Bacterins Prepared by Two Methods

1972

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“…It was discovered that subcutaneous injection of living C. fetus bacteria, could induce immunity in cattle (Plastridge, Kersting et al 1966, Clark 1967. This lead to the development of different commercial vaccines which contained either C. fetus species or subspecies to be used against BGC.…”

Section: Vaccine Developmentmentioning

confidence: 99%

Bovine genital campylobacteriosis: isolation, identification and virulence profiling of Campylobacter fetus subsp. venerealis in a small animal model

Koya¹

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Bovine Genital Campylobacteriosis is a significant disease in Australian beef and dairy herds. The causative organism, Campylobacter fetus subsp. venerealis is a microaerophilic bacterium that often resides asymptomatically in bull penile sheaths and is passed on to females during mating. Virulent strains of the bacterium can cause subfertility and late term abortions in infected females. It has the potential to cause large economic losses and is often difficult to diagnose by culture due to the slow-growing, fastidious bacterium becoming overgrown by contaminants. The gold standard for detection is culture and biochemical testing for identification.Isolation and identification methodologies for C. fetus subsp. venerealis have not been reviewed for many years partly due to the lack of an Australian culture collection. Determining the variation in transport media viability, culture phenotyping, addition of newer phenotypic tests or the evaluation of strain virulence relies on the availability of cultures for analysis. Therefore, the objective of this study was to use a shared culture collection to identify best practice for transport media and isolation methodologies, define the phenotypic profile through refined, standardised methods and develop a pregnant guinea pig model to facilitate future studies of the pathogenicity, abortion mechanism and vaccine efficacy of C. fetus subsp. venerealis.This study biochemically characterised 152 Campylobacter-like isolates to determine the breadth of variability, and to test innovative diagnostic tools for phenotypic characterisations. Strains of C. fetus subsp. venerealis were used to determine viability in the commonly recommended Lander's transport and enrichment medium (TEM) as well as the recently described Thomann transport and enrichment (TTE) medium in vitro and in vivo. The in vitro experiment also aimed to determine the efficacy of the two transport media with subsequent sub-culture onto: sheep blood agar (SBA) with and without filtration (0.45 µm) and Campylobacter selective agar. Incubation temperature effect was also studied, by comparing TEM enrichment at 25°C and 37°C. The in vivo cattle field trial found no significant difference in the isolation of C. fetus subsp. venerealis between Lander's and TTE (p=0.1037), while a higher number of thermophilic Campylobacter cultures were isolated from TTE media. In vitro, both TTE and Lander's media were able to sustain C. fetus subsp. venerealis growth for up to 7 days at either 25°C or 37°C, and allow enrichment in spiked mixed cultures with either P. vulgaris or C. jejuni. Campylobacter selective agar appeared to produce the most consistent positive results from spiked mixed cultures, while SBA was overgrown in most instances and 0.45 µm filtration produced inconsistent results and required a high starting concentration (10 5 CFU/ml). Therefore, diagnostically the use of either TTE or Lander's TEM for transport of samples iii followed by plating onto Campylobacter selective agar was optimal for isolation of ...

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“…Bovine vibriosis has been shown by Donaldson et a1 (1967a) to be one of the factors associated with the low reprodiictive rates that have been found by various workers (Donaldson 1962(Donaldson , 1968Donaldson et ul 1967b;Lamond 1969) in beef cattle in northern Queensland. Clark (1967) and Clark et al (1968a) reported an increascd conception rate in heifers given subcutaneous injections of killed Vibrio fetus var. venerealis incorporated in an adjuvant.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Vaccination Against Bovine Vibriosis on Pregnancy Rates in Heifers in a Northern Queensland Beef Herd

Allan¹,

Mutch

1971

Aust Veterinary J

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Control of Bovine Vibriosis by Vaccination

Cited by 4 publications

References 4 publications

IMMUNISATION AGAINST BOVINE VIBRIOSIS: 1. Comparison of the Protective Properties of Bacterins Prepared by Two Methods

IMMUNISATION AGAINST BOVINE VIBRIOSIS: 1. Comparison of the Protective Properties of Bacterins Prepared by Two Methods

Bovine genital campylobacteriosis: isolation, identification and virulence profiling of Campylobacter fetus subsp. venerealis in a small animal model

The Effect of Vaccination Against Bovine Vibriosis on Pregnancy Rates in Heifers in a Northern Queensland Beef Herd

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