Control of biofilm-producing Aeromonas bacteria in the water tanks and drinkers of broiler poultry farms using chitosan nanoparticle-based coating thyme oil

Mohammed, Asmaa N.; Attia, Amira S.

doi:10.33899/ijvs.2021.131253.1935

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…In addition to mastitis cases, the STEC strains are commonly isolated from severe human diseases (31,32). Raw milk and unpasteurized dairy products (such as soft white cheese) are frequently consumed in Iraq's rural areas (33). This emphasizes the possibility that milk with subclinical mastitis could play a role in introducing the bacterium into the human food chain.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Stx1 and Stx2 genes of E. coli isolated from sub-clinical bovine mastitis in Mosul city

Sheet

Al-Mahmood

Taha

et al. 2023

IJVS

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Subclinical mastitis in cattle is a pathological condition that causes a financial burden on the dairy industry. In addition, it puts the public sector at risk when pathogens of animal origin are present in unpasteurized milk and dairy products that may enter the human food chain and cause serious illnesses. Our study aimed to determine the percentage of pathogenic Escherichia coli causing subclinical mastitis in dairy cows in Mosul city by confirming the existence of Stx1 and Stx2 genes. Eighty milk samples were obtained from cows suffering from subclinical mastitis using sterile procedures from November 2018 to October 2019. Escherichia coli was isolated, characterized, and confirmed using culture and PCR. According to our research, the percentage of pathogenic Escherichia coli in milk samples was 36.3% (29/80). Moreover, molecular screening for a specific Escherichia coli gene revealed that all isolates 100% carried the uidA gene. PCR revealed that 27/29 (93.1%) isolates had both Stx1 + Stx2 genes, and all the isolates 100% possessed the Stx2 gene. No significant relationship was found between the percentage of pathogenic E. coli and the seasons. Even though there were more isolates in the winter than in the summer, this increase was not statistically significant. This study's findings may help pay attention to one of the leading causes of subclinical mastitis, which is beneficial to the private sector to control the disease.

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Section: Discussionmentioning

confidence: 99%

Molecular detection of Stx1 and Stx2 genes of E. coli isolated from sub-clinical bovine mastitis in Mosul city

Sheet

Al-Mahmood

Taha

et al. 2023

IJVS

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“…Aeromonas is also recognized as an important biofilm‐forming waterborne pathogen, commonly prevalent in the DWS (Aravena‐Román et al, 2013; van Der Wielen & Lut, 2016). Biofilm colonization of the DWS (Hahne et al, 2022) serves as a microbial reservoir and increases the load of waterborne pathogens (Mohammed & Attia, 2022). The frequent association of Salmonella in broiler chickens, eggs, and raw poultry meat is a major public health concern and can lead to significant economic losses in the broiler industry (Cosby et al, 2015; de Oliveira et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Continuous production and recirculation of plasma‐activated water bubbles under different flow regimes for mixed‐species bacterial biofilm inactivation inside pipelines

Dhaliwal,

Yang,

Roopesh

2024

Journal of Food Safety

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Biofilm formation in broiler drinking water systems is a public health concern. Bacterial detachment from the pipes into the drinking water subsequently increases the risk of waterborne transmission and has detrimental effects on animal and human health. The study evaluated the antimicrobial effectiveness of plasma‐activated water bubbles (PAWBs) recirculated under different flow regimes against the mixed‐species biofilms of Salmonella Typhimurium ATCC13311 and Aeromonas australiensis 03‐09 grown on the inner surfaces of polyvinyl chloride (PVC) pipes. A benchtop pipeline model representing broiler drinker lines was developed to compare the biofilm inactivation efficacy of PAWB recirculated at different flow rates, corresponding to Reynold's number of 1000, 2500, and 4000. The synergistic mechanical and oxidative recirculation using PAWB resulted in a higher biofilm inactivation from the pipe walls as compared to recirculation using distilled water alone. Apart from the flow regimes, various parameters including the volume of PAWB circulated, the concentration of the major plasma reactive species, and treatment time affected the susceptibility of the mixed‐species biofilms to PAWB treatment. Under all tested conditions, the bacterial cells were below the detection limit of 1 log CFU/mL in water after PAWB treatments. A better understanding of the hydrodynamic variations prevalent in the drinking water system is important for designing an effective disinfection protocol using PAWB. The results obtained from the study provide important information on the use of PAWB for biofilm control strategies.

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“…Bio lms can provide an ideal environment for various pathogenic bacteria, including Aeromonas hydrophila and Pseudomonas aeruginosa, as well as viruses, fungi, and protozoa. These organisms can cause infections and ailments such as gastroenteritis, diarrhea, chronic sinusitis, and food poisoning (Mohammed & Attia, 2022). The formation and adhesion of bio lms is in uenced signi cantly by multiple factors, including the type and density of microorganisms, temperature, pH, disinfectants (both their type and concentration), nutrient availability, hydrodynamic conditions (such as ow rate), and the characteristics of distribution networks.…”

Section: Introductionmentioning

confidence: 99%

Assessment of bacterial communities and residual chlorine dynamics within underground and overhead water storage tanks

Javed,

Amjad,

Hashmi

2023

Preprint

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Biofilms are primary reservoirs for microbial communities within drinking water distribution systems (DWDSs) and exert considerable influence on the overall safety and quality of potable water. The current study centered on investigating the development of biofilms within water storage tanks of National University of Sciences and Technology (NUST) Islamabad, Pakistan. Two types of tank materials, Polyvinyl Chloride (PVC) and Reinforced Cement Concrete (RCC), were selected to study the impact of the material and residual chlorine on biofilm formation. This study analyzed biofilm development on these materials over 15 and 30 days, demonstrating the evolving biofilm dynamics over time. The findings revealed that water samples showed a high degree of bacterial contamination, with biofilm formation on both PVC and RCC surfaces attributed to low residual chlorine levels. The study also scrutinized the differences between PVC and RCC surfaces, highlighting their varied biofilm growth potentials. Bacterial quantity through Heterotrophic Plate Count (HPC) and images obtained from Scanning Electron Microscopy (SEM) showed that RCC surfaces promote denser biofilm growth owing to their roughness. Moreover, most biofilm samples showed more growth over 30 days time period compared at 15 days. Elemental analysis using Energy Dispersive X-ray Spectroscopy (EDX) revealed increased carbon content in biofilms, suggesting the role of organic compounds in microbial nourishment. This study underscores the significance of material selection for water distribution systems with rough surfaces, such as RCC, potentially promote enhanced bacterial attachment and proliferation. Moreover, current findings highlighted the significance of maintaining adequate residual chlorine levels to inhibit biofilm formation.

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Control of biofilm-producing Aeromonas bacteria in the water tanks and drinkers of broiler poultry farms using chitosan nanoparticle-based coating thyme oil

Cited by 5 publications

References 32 publications

Molecular detection of Stx1 and Stx2 genes of E. coli isolated from sub-clinical bovine mastitis in Mosul city

Molecular detection of Stx1 and Stx2 genes of E. coli isolated from sub-clinical bovine mastitis in Mosul city

Continuous production and recirculation of plasma‐activated water bubbles under different flow regimes for mixed‐species bacterial biofilm inactivation inside pipelines

Assessment of bacterial communities and residual chlorine dynamics within underground and overhead water storage tanks

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