Control of benzimidazole-tolerant Penicillium expansum in pome fruit

Koffmann, W.; Penrose, L. J.; Menzies, A.R.; Davis, K.C.; Kaldor, Jill

doi:10.1016/0304-4238(78)90106-1

Cited by 13 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In agreement with our results, reported MICs for resistant isolates were >500 μg ml −1 (Errampalli 2004; Pianzzola et al. 2004) and >1000 μg ml −1 (Koffmann et al. 1978; Sholberg et al.…”

Section: Discussionsupporting

confidence: 93%

“…Although the germ tubes were shortened and twisted as the concentration of fungicide increased, a great number of spores were still considered germinated, as the germ tube was still longer than the diameter of the spores. Koffmann et al. (1978) reported that TBZ at a concentration of 500 μg ml −1 reduced spore germination of sensitive isolates to about half that of the controls; in resistant isolates, TBZ had no effect on percentage germination at any concentration to 1000 μg ml −1 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of methods to detect resistance ofPenicillium expansumto thiabendazole

Cabañas

Abarca

Bragulat

et al. 2009

Letters in Applied Microbiology

View full text Add to dashboard Cite

Aims: Because of the lack of a standard method, the aim of this work is to evaluate the suitability of the broth microdilution method CLSI M38-A in determining the resistance level of some Penicillium expansum isolates to thiabendazole (TBZ). The ability of the isolates to produce patulin (PAT) and citrinin (CIT) has been also assessed. Methods and Results: Penicillium expansum isolates (128) were assayed (apples, pears, grapes and five reference strains). It was observed that 69AE4% of the strains isolated from apples and pears were resistant to TBZ. Sensitive isolates were inhibited at 0AE25-0AE5 lg ml )1 whilst resistant isolates still grew at 512 lg ml )1 . PAT was produced by all P. expansum isolates. CIT was detected in 98AE8% of TBZ-resistant isolates and in 89AE1% of the TBZ-sensitive isolates. Conclusions:The preliminary screening method combined with the adaptation of the method CLSI M38-A, can be a good strategy to be used in assessing the in vitro activity of TBZ against a large number of isolates. Significance and Impact of the Study: The proposed methodology can be a contribution to the standardization of susceptibility tests to fungicides against P. expansum.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Comparison of methods to detect resistance ofPenicillium expansumto thiabendazole

Cabañas

Abarca

Bragulat

et al. 2009

Letters in Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…De hecho, desde hace varios años, existen reportes de cepas de Penicillium expansum resistentes a los Benzimidazoles y en estudios in vitro se demostró la resistencia cruzada con benomilo, carbendazim, thiophanato y thiabendazole (Koffmann et al, 1978). Sin embargo, el uso de fungicidas químicos sintéticos está siendo cada vez más restringido, no solo por la aparición de cepas resistentes, sino debido a los efectos nocivos causados en el ambiente y en la salud pública (Lima et al, 2011).…”

Section: Introductionunclassified

“…In fact, for several years, there are reports of Penicillium expansum strains resistant to benzimidazoles and in vitro studies demonstrated cross-resistance to benomyl, carbendazim, and thiabendazole thiophanato (Koffmann et al, 1978). However, the use of synthetic chemical fungicides is becoming ever more restricted, not only by the emergence of resistant strains, but because of the harmful effects caused to the environment and public health (Lima et al, 2011).…”

Section: Introductionmentioning

confidence: 99%