Control of Bacillus subtilis Replication Initiation during Physiological Transitions and Perturbations

Sauls, John T.; Cox, Sarah; Do, Quynh; Castillo, Victoria del; Ghulam-Jelani, Zulfar; Jun, Suckjoon

doi:10.1128/mbio.02205-19

Cited by 55 publications

(78 citation statements)

References 62 publications

(113 reference statements)

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“…1 that their initiation-centric model is inconsistent with size homeostasis by the adder. We analyzed their simulations and found that they produced the adder-like behavior because of the additional fluctuations in the septum position (Figure S2A).Experimentally, septum position represents the most precise control among all measured single-cell parameters with CV < 5%Sauls et al 2019). Indeed, removing fluctuations in the septum position alone made the simulation deviated from experiment in a quantitative manner consistent with Eq.…”

mentioning

confidence: 56%

Comment on ‘Initiation of chromosome replication controls both division and replication cycles inE. colithrough a double-adder mechanism’

Treut¹,

Si²,

Li³

et al. 2020

Preprint

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Witz et al. recently performed single-cell mother machine experiments to track growth and the replication cycle in E. coli. They analyzed the correlation structure of selected parameters using both their data and published data, and concluded that E. coli cell-size control is implemented at replication initiation, which challenged the newly emerged division-centric mechanism of cell-size control in bacteria. We repeated Witz et al.'s analysis, and performed additional experiments and analytical calculations. These results explain Witz et al.'s observation and in fact support the division-centric model.

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confidence: 56%

Comment on ‘Initiation of chromosome replication controls both division and replication cycles inE. colithrough a double-adder mechanism’

Treut¹,

Si²,

Li³

et al. 2020

Preprint

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“…Quantifying the cell size and growth rates of Salmonella enterica grown in different nutrient media, Schaechter and colleagues discovered the nutrient growth law -bacterial cell size increases exponentially with the population growth rate [2]. High-throughput single-cell studies in recent years have confirmed this result for evolutionary divergent Escherichia coli and Bacillus subtilis [3][4][5][6], suggesting common strategies for bacterial cell size control. However, single-cell data show deviations from the nutrient growth law in experiments altering cellular proteomics [4,7], leaving open the connection between cell size, growth rate and protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Nutrient-dependent trade-offs between ribosomes and division protein synthesis control bacterial cell size and growth

Serbanescu

Ojkic

Banerjee

2020

Preprint

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Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shapes under growth perturbations. Here we develop a whole-cell model for growth dynamics in rod-shaped bacteria that links ribosomal abundance with cell geometry, division control, and the extracellular environment.Our study reveals that cell shape maintenance under nutrient perturbations requires a balanced trade-off between ribosomes and division protein synthesis. Deviations from this trade-off relationship is predicted under translational perturbations, leading to distinct modes of cell morphological changes, in agreement with single-cell experimental data on Escherichia coli. Furthermore, by calibrating our model with experimental data, we predict how combinations of nutrient-, translational-and shape perturbations can be chosen to optimize bacterial growth fitness and drug resistance.

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“…Studying the growth of S. enterica in different nutritional conditions, Schaechter et al discovered the nutrient growth law (5) -cell size increases exponentially with growth rate. Recent single-cell studies have confirmed this result for evolutionary divergent E. coli and B. subtilis (6,7). However, not all cell types change shapes the same way.…”

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confidence: 81%

Bacterial cell shape control by nutrient-dependent synthesis of cell division inhibitors

Ojkic

Banerjee

2021

Preprint

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By analysing cell shape and size of the bacterium Bacillus subtilis under nutrient perturbations, protein depletion, and antibiotic treatments we find that cell geometry is extremely robust, reflected in a well-conserved scaling relation between surface area (S) and volume (V), S~Vγ, with γ=0.85. We develop a molecular model supported by single-cell simulations to predict that the surface-to-volume scaling exponent γ is regulated by nutrient-dependent production of metabolic enzymes that act as cell division inhibitors in bacteria. Using theory that is supported by experimental data, we predict the modes of cell shape transformations in different bacterial species and propose a mechanism of cell shape adaptation to different nutrient perturbations. For organisms with high surface-to-volume scaling exponent γ, such as B. subtilis, cell width is not sensitive to growth rate changes, whereas organisms with low γ, such as A. baumannii, cell shape adapts readily to growth rate changes.

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Control of Bacillus subtilis Replication Initiation during Physiological Transitions and Perturbations

Cited by 55 publications

References 62 publications

Comment on ‘Initiation of chromosome replication controls both division and replication cycles inE. colithrough a double-adder mechanism’

Comment on ‘Initiation of chromosome replication controls both division and replication cycles inE. colithrough a double-adder mechanism’

Nutrient-dependent trade-offs between ribosomes and division protein synthesis control bacterial cell size and growth

Bacterial cell shape control by nutrient-dependent synthesis of cell division inhibitors

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