Control of Antibiotic-Resistant  Salmonella enterica  Serovar Typhi in Water and Milk Using Phage Cocktail

El-Dougdoug, Noha K.; Hassan, Mervet; Elashkar, Eslam; Ahmed, Akhter U.; Hazaa, Mahmoud

doi:10.21608/ejbo.2019.7596.1284

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…Lytic phages treat human bacterial infections (Maura and Debarbieux, 2011), and were used for the decontamination of processed food and agricultural products (Maura & Debarbieux, 2011;El-Shibiny et al, 2017;Huang et al, 2018;Bai et al, 2019). Moreover, El-Dougdoug et al (2020) used lytic phages to control antibiotic resistant Salmonella Typhi in the tap water and milk. Phages have certain benefits, such as high host specificity and self-replication, as long as they are host-present.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Novel Virulent Phages Infecting the Aeromonas Hydrophila and Escherichia coli Isolated from Sewage Water Samples

Nasr-Eldin

Soliman

2020

Egypt. J. Bot.

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M ULTIDRUG-resistant bacteria are now emerging for almost all the present-day antibiotics. Aeromonas hydrophila D2007 and Escherichia coli W102 were isolated from fresh food and drinking water samples and they were resistant to 57.14% and 85.71% of tested common antibiotics respectively. Three bacteriophages (phages) were isolated from sewage samples. Morphological examinations suggested that phage ΦAHP7, which infects A. hydrophila D2007, belongs to the Myoviridae family and other phages ΦECP8 and ΦECP9 capable of lysing E. coil W102 belongs to Siphoviridae and Podoviridae families, respectively. For the three phages, the optimal multiplicity of infection (MOI) was calculated to be 0.001. Phages were characterized by determining their host range and stability in pHs, temperatures, and salinity. The latent periods of phages ΦAHP7, ΦECP8, and ΦECP9 were 10, 20 and 10min with average burst sizes of 53.5±0.5, 26.5±0.5 and 67.5±0.5 phages per infected cell, respectively. The three phages gradually reduced OD 600 and are able to stop the growth of A. hydrophila D2007 and E. coli W102 in vitro at a low MOI of 0.001. Phages ΦAHP7, ΦECP8, and ΦECP9 treatments achieved 1.55, 1.68 and 2.28 log CFU/g (P< 0.01) reduction of viable bacterial number in red cabbage and 1.48, 1.38 and 1.68 log CFU/g (P< 0.01) reduction in tomato after 30 min at room temperature (28°C) respectively. Applications of lytic ΦAHP7, ΦECP8, and ΦECP9 bacteriophages lead to a rapid reduction of A. hydrophila D2007 and E. coli W102 counts in fresh food for human consumption.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Novel Virulent Phages Infecting the Aeromonas Hydrophila and Escherichia coli Isolated from Sewage Water Samples

Nasr-Eldin

Soliman

2020

Egypt. J. Bot.

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“…In particular, phages have been successfully applied to deactivate and control foodborne pathogens in milk, including E. coli [ 44 , 45 ], Salmonella spp. [ 46 , 47 , 48 ], P. lactis [ 49 ], S. aureus [ 50 ], Cronobacter spp. [ 51 , 52 ], Y. enterocolitica [ 53 ], and others.…”

Section: Discussionmentioning

confidence: 99%

A Genomic Analysis of the Bacillus Bacteriophage Kirovirus kirovense Kirov and Its Ability to Preserve Milk

Kazantseva

Skorynina

Piligrimova

et al. 2023

IJMS

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Bacteriophages are widely recognized as alternatives to traditional antibiotics commonly used in the treatment of bacterial infection diseases and in the food industry, as phages offer a potential solution in combating multidrug-resistant bacterial pathogens. In this study, we describe a novel bacteriophage, Kirovirus kirovense Kirov, which infects members of the Bacillus cereus group. Kirovirus kirovense Kirov is a broad-host-range phage belonging to the Caudoviricetes class. Its chromosome is a linear 165,667 bp double-stranded DNA molecule that contains two short, direct terminal repeats, each 284 bp long. According to bioinformatics predictions, the genomic DNA contains 275 protein-coding genes and 5 tRNA genes. A comparative genomic analysis suggests that Kirovirus kirovense Kirov is a novel species within the Kirovirus genus, belonging to the Andregratiavirinae subfamily. Kirovirus kirovense Kirov demonstrates the ability to preserve and decontaminate B. cereus from cow milk when present in milk at a concentration of 104 PFU/mL. After 4 h of incubation with the phage, the bacterial titer drops from 105 to less than 102 CFU/mL.

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Control of Antibiotic-Resistant Salmonella enterica Serovar Typhi in Water and Milk Using Phage Cocktail

Cited by 2 publications

References 37 publications

Evaluation of Novel Virulent Phages Infecting the Aeromonas Hydrophila and Escherichia coli Isolated from Sewage Water Samples

Evaluation of Novel Virulent Phages Infecting the Aeromonas Hydrophila and Escherichia coli Isolated from Sewage Water Samples

A Genomic Analysis of the Bacillus Bacteriophage Kirovirus kirovense Kirov and Its Ability to Preserve Milk

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