Control mechanisms for gonadotrophic hormone production in vitro

Pattillo, Roland A.; Hussa, Robert O.; Delfs, Eleanor; Garancis, John C.; Bernstein, Ronald A.; Rückert, A; Huang, Wei; Gey, George O.; Mattingly, Richard F.

doi:10.1007/bf02617765

Cited by 42 publications

(22 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NTERA-2 cells were also induced to dierentiate by seeding in medium containing 3 mM hexamethylene bisacetamide (HMBA) (Andrews et al, 1990). Other cell lines used were the testicular teratocarcinoma lines TERA-1 (Fogh and Trempe, 1975);833KE, 1156QE and577MF (Andrews et al, 1980) and the choriocarcinoma lines BeWo, (Pattillo and Gey, 1968) and Jar (Pattillo et al, 1971).…”

Section: Cell Culturementioning

confidence: 99%

Human Wnt-13 is developmentally regulated during the differentiation of NTERA-2 pluripotent human embryonal carcinoma cells

1998

View full text Add to dashboard Cite

Section: Cell Culturementioning

confidence: 99%

Human Wnt-13 is developmentally regulated during the differentiation of NTERA-2 pluripotent human embryonal carcinoma cells

1998

View full text Add to dashboard Cite

“…A CC2 cell line was derived from a complete mole whereas the other choriocarcinoma cell lines were established from choriocarcinomas developed from an apparently normal live birth (Bewo, NUC1, JEG3, JAR, NJG), stillbirth (CC3) or abortion (CC1). Growth properties and tumorigenicity of these cells have been documented (Pattillo and Gey, 1968;Pattillo et al, 1971;Suzumori et al, 1983;Kohler and Bridson, 1971;Sugiura et al, 1988;Nozawa et al, 1987). These cell lines were maintained in Dulbecco's modi®ed Eagle's medium (DMEM) or F12 supplemented with 10% fetal bovine serum (FBS) at 378 C in a humidi®ed 5% CO 2 atmosphere.…”

Section: Specimens and Cellsmentioning

confidence: 99%

Human chromosome 7 carries a putative tumor suppressor gene(s) involved in choriocarcinoma

et al. 1997

View full text Add to dashboard Cite

“…Several cell lines (e.g. JAR, JEG and BeWo) have been evaluated for their potential applications in the study of the mechanisms of trans-trophoblast transport of drugs (14)(15)(16). The BeWo cell line is a choriocarcinoma cell line developed from a malignant gestational choriocarcinoma of the fetal placenta and was characterized in 1968 (17).…”

Section: Introductionmentioning

confidence: 99%

Transport and metabolism of opioid peptides across BeWo cells, an in vitro model of the placental barrier

Ampasavate¹,

Chandorkar²,

Velde³

et al. 2002

International Journal of Pharmaceutics

View full text Add to dashboard Cite

. (2002) Transport and metabolism of opioid peptides across BeWo Cells, an in vitro model of the placental barrier. Int. J. Pharm. 233,[85][86][87][88][89][90][91][92][93][94][95][96][97][98] Abstract: In keeping with the advance of biotechnology, cell culture becomes an important tool for investigating the transport and the metabolism phenomena. A cell line of human origin, the BeWo choriocarcinoma cell line, was used for the study of the transport and metabolism of opioid peptides across the in vitro model of the placental barrier. Opioid peptides, both naturally occurring and their synthetic analogs, are of interest to be developed as potent analgesics and were included in this study. The apparent permeability coefficients of the peptides containing 4 to 11 amino acid or analog residues were in the range of 0.23-14.60 x 10 -5 cm/sec. The apparent permeability coefficients of these peptides were comparable to those of sucrose or dextrans, hydrophilic markers. Molecular weight and size of the peptides were inversely correlated to the permeability across the BeWo monolayers. Lipophilicity and charges of the peptides were also investigated and found to be the minor factors regulating the extent of peptide permeation. Contrasting to the transport of DPDPE peptide analog across the blood-brain barrier, the transport of DPDPE across the BeWo monolayers were not found to be via carrier-mediated transport. The major transport pathway of the opioid peptides across the BeWo monolayers was found to be via paracellular route. In metabolism studies, aminopeptidase was found to be a major enzyme type responsible for the degradation of naturally occurring peptides but not for the synthetic analogues. The finding obtained from the present study reveal the applicability of the BeWo cell line to be used as the in vitro cell culture model for the transport and metabolism screening of the opioid peptides.

show abstract

Control mechanisms for gonadotrophic hormone production in vitro

Cited by 42 publications

References 15 publications

Human Wnt-13 is developmentally regulated during the differentiation of NTERA-2 pluripotent human embryonal carcinoma cells

Human Wnt-13 is developmentally regulated during the differentiation of NTERA-2 pluripotent human embryonal carcinoma cells

Human chromosome 7 carries a putative tumor suppressor gene(s) involved in choriocarcinoma

Transport and metabolism of opioid peptides across BeWo cells, an in vitro model of the placental barrier

Contact Info

Product

Resources

About