Control diet in a high-fat diet study in mice: Regular chow and purified low-fat diet have similar effects on phenotypic, metabolic, and behavioral outcomes

Almeida-Suhett, Camila P.; Scott, James N.; Graham, Alice M.; Chen, Yifan; Deuster, Patricia A.

doi:10.1080/1028415x.2017.1349359

Cited by 44 publications

(33 citation statements)

References 35 publications

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“…Changes in fat are influenced by the amount of energy expended versus the amount of energy consumed. Therefore, if energy expenditure is still low, but excessive levels of food consumption, weight gain will occur [34].…”

Section: Resultsmentioning

confidence: 99%

Combined Effects of Weight Training and Aerobic Exercise Accompanied by Normal and Low-Calorie Diets on Fat Percentage of Young Women

Padli¹,

Kiram²,

Syahara³

et al. 2020

saj

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Section: Resultsmentioning

confidence: 99%

Combined Effects of Weight Training and Aerobic Exercise Accompanied by Normal and Low-Calorie Diets on Fat Percentage of Young Women

Padli¹,

Kiram²,

Syahara³

et al. 2020

saj

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“…Luminal glucose concentrations in the small intestine in humans are approximately 50-500 mM and exceed 300 mM after a meal. For animals on nearly physiological diets, the luminal glucose concentrations averaged 0.4-24 mM [7]. Thus, luminal glucose may be an endogenous carbon source for L. mesenteroides EH-1.…”

Section: Discussionmentioning

confidence: 99%

“…Conversely, obesity further worsens the dysbiosis of gut microbiota, forming a vicious circle. Thus, the rectification of gut dysbiosis has become a promising strategy to treat obesity by drug or nutritional intervention [7]. Short-chain fatty acids (SCFAs), the main fermentation metabolites of probiotic bacteria such as butyric acid, have been shown to reduce HFD-induced metabolic disorders and inflammation [8].…”

Section: Introductionmentioning

confidence: 99%

Mouse Abdominal Fat Depots Reduced by Butyric Acid-Producing Leuconostoc mesenteroides

et al. 2020

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The activation of peroxisome proliferator-activated rece ptor gamma (PPAR-γ) is known to induce the differentiation of adipocytes. This study aimed to investigate the probiotic effect of Leuconostoc mesenteroides (L. mesenteroides) on high-fat diet (HFD)-induced PPAR-γ activation and abdominal fat depots. Incubation of differentiated 3T3-L1 adipocytes with media of L. mesenteroides EH-1, a butyric acid-producing strain, significantly reduced the amounts of lipid droplets. The oral administration of L. mesenteroides EH-1 produced large amounts (>1 mM) of butyric acid in cecum and attenuated the HFD-induced upregulation of PPAR-γ and accumulation of abdominal fats in mice. The combination of 2% glucose with L. mesenteroides EH-1 increased the production of butyric acid and potentiated the probiotic activity of L. mesenteroides EH-1 against the formation of lipid droplets in 3T3-L1 adipocytes as well as abdominal fats in HFD-fed mice. The inhibition of free fatty acid receptor 2 (Ffar2) by its antagonist, GLPG-0974, markedly diminished the probiotic effects of L. mesenteroides EH-1 plus glucose on the suppression of HFD-induced PPAR-γ and abdominal fats. Besides demonstrating the probiotic value of L. mesenteroides EH-1, our results highlight the possible therapy targeting the butyric acid-activated Ffar2 pathway to reduce abdominal fats.

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“…As a result, the two groups fed with the control diets develop similar metabolic and behavioral profiles except for total cholesterol levels, which was significantly higher in purified low fat diet mice compared to chow diet mice. [81] Thus, a low-fat purified diet is a more appropriate control diet for these experiments than the chow diet, once they can differ not only in relative amounts of fat and carbohydrate but also in nutritional composition and source of dietary fibers. [59,82,83] Finally, it is essential to highlight that the animals' exposure to HFDs can alter many other systems as gut microbiome, inflammatory and neuroinflammatory responses, increase tumorigenesis and risk of cancer, promote behavioral and reproductive disorders, independent of obesity phenotype and major metabolic changes, so researches need to be aware of these alterations.…”

Section: Types Of Dietsmentioning

confidence: 99%

Modeling Diet‐Induced Metabolic Syndrome in Rodents

Leonardi

Gosmann

Zimmer

2020

Molecular Nutrition Food Res

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Standardized animal models represent one of the most valuable tools available to understand the mechanism underlying the metabolic syndrome (MetS) and to seek for new therapeutic strategies. However, there is considerable variability in the studies conducted with this essential purpose. This review presents an updated discussion of the most recent studies using diverse experimental conditions to induce MetS in rodents with unbalanced diets, discusses the key findings in metabolic outcomes, and critically evaluates what we have been learned from them and how to advance in the field. The study includes scientific reports sourced from the Web of Science and PubMed databases, published between January 2013 and June 2020, which used hypercaloric diets to induce metabolic disorders, and address the impact of the diet on metabolic parameters. The collected data are used as support to discuss variables such as sex, species, and age of the animals, the most favorable type of diet, and the ideal diet length to generate metabolic changes. The experimental characteristics propose herein improve the performance of a preclinical model that resembles the human MetS and will guide researchers to investigate new therapeutic alternatives with confidence and higher translational validity.

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Control diet in a high-fat diet study in mice: Regular chow and purified low-fat diet have similar effects on phenotypic, metabolic, and behavioral outcomes

Abstract: Our data demonstrate that young mice develop similar phenotypic, metabolic, and behavioral profiles when fed SD vs. LFD. The two diets may thus be equally appropriate as controls for an HFD, although some studies may want to consider differences in effects on cholesterol levels.

Cited by 44 publications

References 35 publications

Combined Effects of Weight Training and Aerobic Exercise Accompanied by Normal and Low-Calorie Diets on Fat Percentage of Young Women

Combined Effects of Weight Training and Aerobic Exercise Accompanied by Normal and Low-Calorie Diets on Fat Percentage of Young Women

Mouse Abdominal Fat Depots Reduced by Butyric Acid-Producing Leuconostoc mesenteroides

Modeling Diet‐Induced Metabolic Syndrome in Rodents

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