Control de la chinche de los pastos Collaria scenica (Hemiptera: Miridae) con nematodos entomopatógenos en invernadero

Abstract

RESUMENSe estudió la eficacia de juveniles infectivos (JI) de Steinernema sp. JCL024 y Heterorhabditis sp. SL0708 sobre adultos y ninfas de Collaria scenica Stal bajo condiciones de invernadero. Se evaluaron 5 dosis (2, 16, 78, 160 JIs)/cm 2 foliolo) para cada nematodo. El experimento fue observado durante 21 días cada 24 horas, realizando el conteo de mortalidad y valoración del daño del pasto kikuyo. Las chinches presentaron 100 % de mortalidad después de 15 días. Cada chinche produjo en promedio 13.000 JI/6 días. En cuanto al daño, no alcanzó el nivel 2 en los 21 días respecto al tratamiento sin nematodos que presentó daño 3 con necrosis y enrollamiento apical de las hojas. Por ende, Steinernema sp. JCL024 y Heterorhabditis sp. SL0708 ejercieron control en la chinche de los pastos y mantuvieron bajo el daño en el follaje.Palabras clave: control biológico, juvenil infectivo, Steinernema sp., Heterorhabditis sp., Pennisetum clandestinum Control of pasture Collaria scenica bug (Hemiptera: Miridae) with entomopathogenic nematodes in greenhouse ABSTRACTWe studied the efficacy of infective juveniles (IJ) of Steinernema sp. strain JCL024 and Heterorhabditis sp. strain SL0708 on adults and nymphs of Collaria scenica under greenhouse conditions. Five doses were evaluated (2, 16, 78, 160 IJ) cm -2 leaf) for each nematode. The experiment was evaluated for 21 days every 24 hours, making mortality counts and assessment of damage on Kikuyo grass. The bugs showed 100% mortality after 15 days. Each bug produced an average of 13,000 IJ/6 days. As for the damage did not reach level 2 within 21 days prior to treatment without nematodes that damage introduced 3 with necrosis and apical leaf curl. Thus, Steinernema sp. strain JCL024 and Heterorhabditis sp. strain SL0708 exercised control bug on the grass and kept under the level of damage to the foliage.

Assessment of entomopathogenic nematodes and their symbiotic bacteria to control the stink bugs Euschistus heros and Dichelops melacanthus (Heteroptera: Pentatomidae) in the soybean-corn succession system

“…There have been some reports of EPNs serving as biological control agents against Hemiptera species on the leaves (Martínez-Córdoba et al, 2011;Naranjo et al, 2013;Niekerk and Malan, 2013;Platt et al, 2020), trunks/stems/shoots (Shapiro-Ilan and Mizell, 2012;Marrero et al, 2015;Guide et al, 2015;Guide et al, 2019) and soil (Leite et al, 2005;Melo et al 2006;Batista and Auad, 2010;Vieux and Malan, 2013;Mbata and Shapiro-Ilan, 2013;Batista et al, 2014;Alves et al, 2009;Guerrero & Pardey, 2019;Moussa et al, 2021;Zart et al, 2021). Susceptibilities of these insects range widely, with some species being more resistant than other usually tested insect hosts, requiring high rate of EPNs (100 IJs/cm 2 ) to provide > 70% mortality (Guide et al, 2015;Guide et al, 2019).…”

Assessment of Entomopathogenic Nematodes in Oil Emulsions to Control Scyphophorus acupunctatus in Agave under Laboratory Conditions

“…In Collaria scenica nymphs inoculated with S. glaseri, 24 h after infection, 75% mortality was found, and at 48 h, mortality was 82% with the nematode Heterorhabditis sp. [23].…”