CONTROL BIOLÓGICO DEL “cogollero del maíz” Spodoptera frugiperda, (Lepidoptera; Noctuidae) CON EL BACULOVIRUS SfVPN, EN IQUITOS-PERU

Vásquez, Joel; Zeddam, Jean-Louis; Tresierra-Ayala, Álvaro

doi:10.24841/fa.v13i1-2.132

Cited by 12 publications

(6 citation statements)

References 4 publications

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“…The median lethal dose was similar between tested isolates and strains, except for the native isolate SfCH32, which showed the lowest LD 50 and was 1.14, 1.20 and 1.43 times more virulent than SfCH15, SfAN1 and SfNIC, respectively. The LD 50 values were also lower than those reported by Vásquez, Zeddam, and Tresierra () using a dose of 4.97 × 10 4 OBs/larva in third instar FAW larvae, being the isolates SfCH32 and SfCH15, 89 and 78 times more virulent, respectively. On the other hand, Rangel Núñez et al, () tested three exotic nucleopolyhedrovirus strains from Argentina, USA and Honduras against a Mexican population of FAW, where the Argentine strain was the most virulent with a LC 50 of 4.9 × 10 4 OBs/ml on first instar larvae.…”

Section: Discussioncontrasting

confidence: 58%

Molecular and Morphological characterization of multiple nucleopolyhedrovirus from Mexico and their insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

Ordóñez-García

Rios‐Velasco

Ornelas‐Paz

et al. 2019

J Applied Entomology

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The fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae), is the most important pest of maize in many countries. Entomopathogenic viruses mainly Baculoviruses family are excellent biological control agents and therefore a viable alternative for managing this pest. The aim of this study was to determine the biological activity of eight native nucleopolyhedrovirus (NPVs) against FAW larvae.Additionally, two of the most virulent isolates (SfCH32 and SfCH15) were characterized biologically by bioassays to estimate their median lethal dose (LD 50 ) and median lethal time (LT 50 ), morphologically by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) and molecularly by restriction enzymes.Three (SfCH15, SfCH18 and SfCH32) of the eight tested native nucleopolyhedrovirus isolates caused mortalities ˃98% at 168-hr post-inoculation (hpi) with a dose of 9.2 × 10 4 OBs/larva. SfCH15 and SfCH32 isolates showed occlusion bodies (OBs) of irregular shape and size (1.02-2.24 μm). The SfCH15 and SfCH32 isolates showed similar median lethal dose (5.6 × 10 2 -6.4 × 10 2 OBs/larva). The lowest median lethal time (114.5 hpi) was observed with the SfCH15 isolate at the highest concentration (2.5 × 10 6 OBs/larva). The DNA restriction profiles for SfCH15 and SfCH32 were different, with their genome size being ~128,000 bp and 132,000 bp, respectively. SfCH15 and SfCH32 isolates showed similar morphological characteristics and the highest virulence against fall armyworm. This study showed that native isolates were highly virulent against S. frugiperda larvae, being similar to other reported strains; however, field studies are required to confirm their insecticidal effect. K E Y W O R D Sbaculovirus, biological control, fall armyworm, genetic variability, morphology, virulence

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Section: Discussioncontrasting

confidence: 58%

Molecular and Morphological characterization of multiple nucleopolyhedrovirus from Mexico and their insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

Ordóñez-García

Rios‐Velasco

Ornelas‐Paz

et al. 2019

J Applied Entomology

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“…The type of research was pre-experimental, the effect of crossing specimens of the species Spodoptera sunia was characterized in order to strengthen the genetic material of the breeding of Spodoptera sunia insects used as a host in the production of the NPV (Vásquez et al, 2002;Salinas-Sánchez et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Cross-species characterization in the reproduction of Spodoptera sunia (Lepidoptera: Noctuidae)

Real-Baca

Zúniga-González

2023

F1000Res

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Background: The research focused on evaluating the biological and reproductive parameters of the species Spodoptera sunia with the introduction of field genetic material, in the Noctuid Insect Breeding Laboratory. Methods: The study was pre-experimental using three treatments with 30 individuals and three repetitions. The individuals were collected from the field, transferred to the laboratory under semi-controlled conditions of temperature and humidity, later they were quarantined for up to three generations for the assembly of the test where the crossing was carried out. In the measurement of the biological and reproductive parameters. Results: The results of the treatments showed that the biological and reproductive parameters in relation to the number of pupae were T2 34 males and 26 females, T3 was 33 males, and 27 females, T1 obtained 27 males and 33 females. The average weight in female T1 was 0.2112 mg and T2 was 0.2401 mg. The number of eggs in T1 in nine days oviposited 196 egg masses, in T2 in seven days 59 egg masses were oviposited, and in T3 160 egg masses were oviposited. In the length parameter in mm T3 obtained 30 mm in larval development, T1 and T2 obtained 27 mm. Finally, in the development stages, the number of days was for T1 and T2, 24 days and for T3 18 days. In the adult stages T1 and T2 it was 12 days and for T3 10 days. In the egg stage in the three treatments it was three days and the pupal stage was eight days. Conclusions: It is concluded that T2 and T3 presented the most optimal results. It is recommended to introduce genetic material every six months to maintain a good production of larvae of the species under study in laboratories for the production and reproduction of insect breeding.

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“…In this context, the use of baculoviruses seems to be a promising alternative. Countries like Argentina [ 116 , 117 ], Brazil [ 118 , 119 , 120 ], Colombia [ 121 ], Honduras [ 122 ], Mexico [ 123 , 124 , 125 , 126 ], Peru [ 127 ], and Venezuela [ 128 ] have evaluated the effectiveness of geographical variants of SfMNPV in laboratory or field conditions, against local populations of S . frugiperda .…”

Section: Examples Of Baculovirus Control Programs In Latin Americamentioning

confidence: 99%

Baculovirus Insecticides in Latin America: Historical Overview, Current Status and Future Perspectives

Haase

Sciocco-Cap

Romanowski

2015

Viruses

173

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Baculoviruses are known to regulate many insect populations in nature. Their host-specificity is very high, usually restricted to a single or a few closely related insect species. They are amongst the safest pesticides, with no or negligible effects on non-target organisms, including beneficial insects, vertebrates and plants. Baculovirus-based pesticides are compatible with integrated pest management strategies and the expansion of their application will significantly reduce the risks associated with the use of synthetic chemical insecticides. Several successful baculovirus-based pest control programs have taken place in Latin American countries. Sustainable agriculture (a trend promoted by state authorities in most Latin American countries) will benefit from the wider use of registered viral pesticides and new viral products that are in the process of registration and others in the applied research pipeline. The success of baculovirus-based control programs depends upon collaborative efforts among government and research institutions, growers associations, and private companies, which realize the importance of using strategies that protect human health and the environment at large. Initiatives to develop new regulations that promote the use of this type of ecological alternatives tailored to different local conditions and farming systems are underway.

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CONTROL BIOLÓGICO DEL “cogollero del maíz” Spodoptera frugiperda, (Lepidoptera; Noctuidae) CON EL BACULOVIRUS SfVPN, EN IQUITOS-PERU

Cited by 12 publications

References 4 publications

Molecular and Morphological characterization of multiple nucleopolyhedrovirus from Mexico and their insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

Molecular and Morphological characterization of multiple nucleopolyhedrovirus from Mexico and their insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

Cross-species characterization in the reproduction of Spodoptera sunia (Lepidoptera: Noctuidae)

Baculovirus Insecticides in Latin America: Historical Overview, Current Status and Future Perspectives

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