Control and maintenance of mammalian cell size

Cooper, Stephen

doi:10.1186/1471-2121-5-35

Cited by 46 publications

(16 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These mechanisms are distinguishable through analysis of DNA content and cell size (reviewed in (Cooper, 2004)). To determine which of these mechanisms contribute to the proliferative arrest of TRPM7-deficient cells cultured in regular media, we analyzed the DNA content (via DAPI staining) and cell size (via forward scatter) of TRPM7-deficient cells upon transition from proliferation in growth supporting Mg 2+ -supplemented media to regular media (Figure 1A).…”

Section: Resultsmentioning

confidence: 99%

TRPM7 Ion Channels Are Required for Sustained Phosphoinositide 3-Kinase Signaling in Lymphocytes

2008

View full text Add to dashboard Cite

Summary Lymphocytes lacking the TRPM7 dual function ion channel/protein kinase exhibit a unique phenotype: they are unable to proliferate in regular media, but proliferate normally in media supplemented with 10–15 mM extracellular Mg2+. Here, we have analyzed the molecular mechanisms underlying this phenotype. We find that upon transition from proliferation-supporting Mg2+-supplemented media to regular media, TRPM7-deficient cells rapidly downregulate their rate of growth, resulting in a secondary arrest in proliferation. The downregulated growth rate of transitioning cells is associated with a deactivation of signaling downstream from phosphoinositide 3-kinase, and expression of constitutively active p110 phosphoinositide 3-kinase is sufficient to support growth and proliferation of TRPM7-deficient cells in regular media. Together, these observations indicate that TRPM7 channels are required for sustained phosphoinositide 3-kinase-dependent growth signaling, and therefore that TRPM7 is positioned alongside phosphoinositide 3-kinases as a central regulator of lymphocyte growth and proliferation.

show abstract

Section: Resultsmentioning

confidence: 99%

TRPM7 Ion Channels Are Required for Sustained Phosphoinositide 3-Kinase Signaling in Lymphocytes

2008

View full text Add to dashboard Cite

show abstract

“…Our model assumed the EGF signaling pathway in a mammalian cell in which the volume is typically > 10 −12 L, which is much larger than yeast or bacteria (10 −16 –10 −14 L) [45,46]. Therefore, a mammalian cell has a huge number of molecules even if the same concentrations of proteins are present in yeast and bacterial cells.…”

Section: Discussionmentioning

confidence: 99%

Modeling Cellular Noise Underlying Heterogeneous Cell Responses in the Epidermal Growth Factor Signaling Pathway

Iwamoto¹,

Shindo²,

Takahashi

2016

PLoS Comput Biol

View full text Add to dashboard Cite

Cellular heterogeneity, which plays an essential role in biological phenomena, such as drug resistance and migration, is considered to arise from intrinsic (i.e., reaction kinetics) and extrinsic (i.e., protein variability) noise in the cell. However, the mechanistic effects of these types of noise to determine the heterogeneity of signal responses have not been elucidated. Here, we report that the output of epidermal growth factor (EGF) signaling activity is modulated by cellular noise, particularly by extrinsic noise of particular signaling components in the pathway. We developed a mathematical model of the EGF signaling pathway incorporating regulation between extracellular signal-regulated kinase (ERK) and nuclear pore complex (NPC), which is necessary for switch-like activation of the nuclear ERK response. As the threshold of switch-like behavior is more sensitive to perturbations than the graded response, the effect of biological noise is potentially critical for cell fate decision. Our simulation analysis indicated that extrinsic noise, but not intrinsic noise, contributes to cell-to-cell heterogeneity of nuclear ERK. In addition, we accurately estimated variations in abundance of the signal proteins between individual cells by direct comparison of experimental data with simulation results using Apparent Measurement Error (AME). AME was constant regardless of whether the protein levels varied in a correlated manner, while covariation among proteins influenced cell-to-cell heterogeneity of nuclear ERK, suppressing the variation. Simulations using the estimated protein abundances showed that each protein species has different effects on cell-to-cell variation in the nuclear ERK response. In particular, variability of EGF receptor, Ras, Raf, and MEK strongly influenced cellular heterogeneity, while others did not. Overall, our results indicated that cellular heterogeneity in response to EGF is strongly driven by extrinsic noise, and that such heterogeneity results from variability of particular protein species that function as sensitive nodes, which may contribute to the pathogenesis of human diseases.

show abstract

“…A logarithmic fit was used to determine the equation that permits calculating the concentration in the whole cell lysates. Finally, an estimation of cell volume70 was used to determine the concentration of DWNTs per cell.…”

Section: Methodsmentioning

confidence: 99%

Uptake and Release of Double‐Walled Carbon Nanotubes by Mammalian Cells

Neves

Heister

Costa

et al. 2010

Adv Funct Materials

View full text Add to dashboard Cite

Efforts to develop carbon nanotubes (CNTs) as nano‐vehicles for precise and controlled drug and gene delivery, as well as markers for in vivo biomedical imaging, are currently hampered by uncertainties with regard to their cellular uptake, their fate in the body, and their safety. All of these processes are likely to be affected by the purity of CNT preparation, as well as the size and concentration of CNTs used, parameters that are often poorly controlled in biological experiments. It is demonstrated herein that under the experimental conditions of standard transfection methods, DWNTs are taken up by cultured cells but are then released after 24 h with no discernable stress response. The results support the potential therapeutic use of CNTs in many biomedical settings, such as cancer therapy.

show abstract

Control and maintenance of mammalian cell size

Cited by 46 publications

References 39 publications

TRPM7 Ion Channels Are Required for Sustained Phosphoinositide 3-Kinase Signaling in Lymphocytes

TRPM7 Ion Channels Are Required for Sustained Phosphoinositide 3-Kinase Signaling in Lymphocytes

Modeling Cellular Noise Underlying Heterogeneous Cell Responses in the Epidermal Growth Factor Signaling Pathway

Uptake and Release of Double‐Walled Carbon Nanotubes by Mammalian Cells

Contact Info

Product

Resources

About