Contributions to the physiology of the isolated heart

Locke, F. S.; Rosenheim, Otto

doi:10.1113/jphysiol.1907.sp001229

Cited by 114 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other investigators (36) found a similar relationship in isolated heart preparations; under conditions of normoxia and adequate perfusion, there was no lactate production in these preparations. Thus, myocardial lactate release or production is often equated with hypoxia or ischemia.…”

Section: Discussionsupporting

confidence: 63%

Metabolic fate of extracted glucose in normal human myocardium.

Wisneski¹,

Gertz²,

Neese³

et al. 1985

J. Clin. Invest.

181

View full text Add to dashboard Cite

Glucose is an important substrate for myocardial metabolism. This study was designed to determine the effect of circulating metabolic substrates on myocardial glucose extraction and to determine the metabolic fate of glucose in normal human myocardium. Coronary sinus and arterial catheters were placed in 23 healthy male volunteers. 16-"'CjGlucose was infused as a tracer in 10 subjects. 16-'CiGlucose and IU-_3Cilactate were simultaneously infused in the other 13 subjects. Simultaneous blood samples were obtained for chemical analyses of glucose, lactate, and free fatty acids and for the isotopic analyses of glucose and lactate. Glucose oxidation was assessed by measuring myocardial 4 CO2 production. The amount of glucose extracted and oxidized by the myocardium was inversely correlated with the arterial level of free fatty acids (r = -0.71; P < 0.0001). 20% (range, 0-63%) of the glucose extraction underwent immediate oxidation. Chemical lactate analysis showed a net extraction of 26.0±16.4%. However, isotopic analysis demonstrated that lactate was being released by the myocardium. In the 13 subjects receiving the dual-carbon-labeled isotopes, the lactate released was 0.09±0.04 ,mol/ml and 49.5±29.5% of this lactate was from exogenous glucose. This study demonstrates that the circulating level of free fatty acids plays a major role in determining the amount of glucose extracted and oxidized by the normal human myocardium. Only 20.1±19.4% of the glucose extracted underwent oxidation, and 13.0±9.0% of the glucose extracted was metabolized to lactate and released by the myocardium. Thus, 60-70% of the glucose extracted by the normal myocardium is probably stored as glycogen in the fasting, resting state.

show abstract

Section: Discussionsupporting

confidence: 63%

Metabolic fate of extracted glucose in normal human myocardium.

Wisneski¹,

Gertz²,

Neese³

et al. 1985

J. Clin. Invest.

181

View full text Add to dashboard Cite

show abstract

“…This is said to be the first instance of in vitro cultivation of animal tissue. Balanced salt solutions were developed one after another in the wake of Ringer's report, including Locke's solution,6 Tyrode's solution,7 the Krebs–Ringer bicarbonate solution,8 Gey's solution,9 Earle's solution,10 and Hanks' solution 11. The composition of these balanced salt solutions is simple and includes only inorganic salts, sometimes with glucose added as a nutrient.…”

Section: History Of Cell Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

376

278

View full text Add to dashboard Cite

BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

show abstract

“…(The effectiveness of these precautions was always controlled by preparing agar plates from the final solution.) The solution so obtained was identical in its composition with that described by Locke and Rosenheim (5), and had the same pH, but was also sterile.…”

mentioning

confidence: 84%

The action of insulin on the aseptically perfused heart

Bodo¹,

Marks²

1927

The Journal of Physiology

View full text Add to dashboard Cite

Cousy (1), of Noyon's laboratory, has recently repeated the perfusion experiments of Hepburn and Latchford(2) on the isolated rabbit's heart, in which they observed an increase in apparent sugar disappearance following the administration of insulin. But, whereas under ordinary conditions, the former observes a similar increase, when aseptic precautions are taken, he finds the sugar consumption to be practically abolished, instead of increased. Thus, in a number of experiments performed in the ordinary way, the apparent sugar disappearance was increased on the average from 1-08 to 1l85 mgr. per gr. of heart per hour under the action of insulin, while in similar aseptic experiments it was reduced from 0-85 to 0O15 mgr. per gr. per hour.From these results Cousy concludes that insulin inhibits the glycolytic action of the heart, and attributes the increase in sugar disappearance normally observed after insulin to the action of bacterial contamination.

show abstract

Contributions to the physiology of the isolated heart

Cited by 114 publications

References 0 publications

Metabolic fate of extracted glucose in normal human myocardium.

Metabolic fate of extracted glucose in normal human myocardium.

Animal‐cell culture media: History, characteristics, and current issues

The action of insulin on the aseptically perfused heart

Contact Info

Product

Resources

About