Contributions to the knowledge of Pseudolevinseniella (Trematoda: Digenea) and temnocephalans from alien crayfish in natural freshwaters of Thailand

Abstract

Redclaw crayfish (Cherax quadricarinatus) is a decapod species originating from Australian freshwater. For more than two decades, these crayfish have been re-distributing to environments in many countries, including Thailand. Moreover, they can carry endosymbionts and/or ectosymbionts into new environments. The aim of this study was to introduce a morphological description of Pseudolevinseniella anenteron as a metacercaria of the endoparasites of redclaw crayfish collected from natural water sources in Thailand. The occurrence of two ectosymbiotic temnocephalans (Diceratocephala boschmai and Temnosewellia sp.) in C. quadricarinatus was also reported. The internal morphology of P. anenteron, D. boschmai and Temnosewellia were described and discussed. The surface ultrastructure of the multidentate spines on the body and the metacercarial cyst wall of P. anenteron was investigated by scanning electron microscopy (SEM). By performing a search of the GenBank nucleotide database of partial sequences of 18S, 28S rDNA and cytochrome c oxidase subunit I (cox1), P. anenteron was found to be related to Maritrema, and Temnosewellia was found to be related to T. fasciata. However, according to the cox1 gene, Temnosewellia was found to be similar to T. minor. These results reveal that redclaw crayfish that inhabit natural freshwaters in Thailand may harbour endoparasites and ecto- and endosymbionts. Furthermore, these findings may be able to monitor invasive or non-invasive species in an ecosystem.

Temnosewellia aff. vietnamensis (Platyhelminthes: Rhabdocoela: Temnocephalidae) associated with freshwater crabs from Kagoshima, southern Japan, with review of records of the genus from East to South Asian countries

“…Together with the host, the species of Temnosewellia might be invaded. Just as Ngamniyom et al (2019) claimed that the species genetically similar to T. minor and T. fasciata , the species was formed a clade with T. minor by the partial sequences of COI in this study (fig. 6).…”

“…Black filled star = Temnosewellia aff. vietnamensis newly collected from the Osumi Peninsula, Kagoshima, Japan in this study; open star = same from the Osumi Peninsula, Kagoshima, Japan by Okawachi et al (2013); black filled triangle = Temnosewellia minor from Ibusuki, Kagoshima, Japan (Tamura et al , 1985; Oki et al , 1995); open circle = Temnosewellia semperi from Sumatra, Java, and various sites of Sulawesi, Indonesia (Weber, 1889), Luzon and Mindanao, Philippines (Semper, 1872), Malaysia (Rohde, 1966; Cannon, 1991), Dawna Hills and Dawei, Myanmar (Gravely, 1913), Narmada River in Madhya Pradesh, Jaintia Hills, and Manipur Hills, India (Gravely, 1913; Chauhan & Ramakrishna, 1953), Yembung and Siyom Rivers in Abor Country (Wood-Mason, 1875; Gravely, 1913; Chauhan & Ramakrishna, 1953), and Yunnan and Foochow, southern China (Gravely, 1913; Lee, 1936); black filled square = Temnosewellia rouxi from Aru Island, Indonesia (Merton, 1914); black filled circle = Temnosewellia vietnamensis from Quangnam, Vietnam (Damborenea & Brusa, 2009); and open triangle = unidentified species of Temnosewellia from Tokushima, Saga, Miyazaki, Kagoshima, Tanegashima Island, Okinawa Island, Ishigaki Island, and Iriomote Island, Japan (Okada, 1938; Suzuki et al , 1983; Shimazu, 2003; Iwata et al , 2004; Kawakatsu et al , 2007a), Taipei, Taiwan (Okada, 1938), Lombok, Indonesia (Ng & Anker, 2016), and eastern Thailand (Ngamniyom et al , 2019). …”

“…From Taiwan, Okada (1938) found a congener from Yanmingshan, Taipei, and he mentioned that the species is conspecific to the unidentified congener from Okinawa Island, Japan. In eastern Thailand, an unidentified species of Temnosewellia was found from the red claw crayfish Cherax quadricarinatus von Martens, 1868 introduced from Australia (Ngamniyom et al , 2019). Together with the host, the species of Temnosewellia might be invaded.…”

The redclaw crayfish: A prominent aquaculture species with invasive potential in tropical and subtropical biodiversity hotspots

“…heavy metals) in redclaw, thus possibly representing health risk when consumed (Nakayama et al . 2010); however, evidence that invasive crayfish act substantially different when compared with ecologically relevant native counterparts remains missing (Kouba et al . 2010).…”