Contributions of the Direct and Indirect Effects of Ionizing Radiation to Reproductive Cell Death

Korystov, Yu. N.

doi:10.2307/3578162

Cited by 28 publications

(10 citation statements)

References 19 publications

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“…The indirect effects of gamma irradiation are dampened by scavengers, molecules that react with hydroxyl radicals and ozone, thereby preventing their ability to act on viral nucleic acids or proteins. While some studies report that proteins in solution (the most common scavengers found in biological samples) have a negative impact on gamma inactivation [ 6 , 12 , 18 ], other studies suggest that protein content does not alter the efficacy of gamma inactivation, particularly on frozen samples [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of RNA Viruses by Gamma Irradiation: A Study on Mitigating Factors

Hume

Ames

Rennick

et al. 2016

Viruses

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Effective inactivation of biosafety level 4 (BSL-4) pathogens is vital in order to study these agents safely. Gamma irradiation is a commonly used method for the inactivation of BSL-4 viruses, which among other advantages, facilitates the study of inactivated yet morphologically intact virions. The reported values for susceptibility of viruses to inactivation by gamma irradiation are sometimes inconsistent, likely due to differences in experimental protocols. We analyzed the effects of common sample attributes on the inactivation of a recombinant vesicular stomatitis virus expressing the Zaire ebolavirus glycoprotein and green fluorescent protein. Using this surrogate virus, we found that sample volume and protein content of the sample modulated viral inactivation by gamma irradiation but that air volume within the sample container and the addition of external disinfectant surrounding the sample did not. These data identify several factors which alter viral susceptibility to inactivation and highlight the usefulness of lower biosafety level surrogate viruses for such studies. Our results underscore the need to validate inactivation protocols of BSL-4 pathogens using “worst-case scenario” procedures to ensure complete sample inactivation.

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Section: Introductionmentioning

confidence: 99%

Inactivation of RNA Viruses by Gamma Irradiation: A Study on Mitigating Factors

Hume

Ames

Rennick

et al. 2016

Viruses

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“…1). In the light of theoretical models of cell survival (7,8) Radiation-induced cell damage are in 80 to 90 % caused by the products of water radiolysis (13). Under conditions used in this work the principal products of water radiolysis are "OH and Oz" radicals.…”

Section: Discussionmentioning

confidence: 96%

Effect of dose‐rate and dose fractionation on radiation‐induced hemolysis of human erythrocytes

1999

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SummaryHuman erythrocytes suspended in an isotonic Na-phosphate buffer, pH 7.4 (hematocfit 2 %) were exposed under air to gamma radiation at a dose rates of 2.2 kCry-h "l and 4.2 kGy.h "1. The dose-response curves for hemolysis of erythrocytes indicated that the process of hemolysis is inversely related to the dose-rate. At both dose-rates we observed a reduced level of hemolysis, when erythrocytes were irradiated with a split dose (0.4 kGy + 2.3 kGy with an interval time between the subsequent exposures from 1 to 4 h) in comparison with the same single dose (2.7 kGy). The maximal effect of fractionation was observed when the interfraction time was equal to 3.5 h. The influence of the interfraction temperature on this effect was observed. The results obtained indicate that enucleated human erythrocytes under suitable radiation conditions are capable of repairing radiation damage which leads to hemolysis.

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“…Scavengers are unable to prevent semi-direct effect due to the hydroxyl radicals from the bound water since the water lattice around DNA does not possess any solvent power (Korystov, 1992). Therefore, scavenging of the radicals from the bound water by an exogenous protector is almost impossible.…”

Section: Effect Of Temperature and Additive On Radiosensitivity Of LImentioning

confidence: 99%

Sterilization by Gamma Irradiation

Aquino¹

2012

Gamma Radiation

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Contributions of the Direct and Indirect Effects of Ionizing Radiation to Reproductive Cell Death

Cited by 28 publications

References 19 publications

Inactivation of RNA Viruses by Gamma Irradiation: A Study on Mitigating Factors

Inactivation of RNA Viruses by Gamma Irradiation: A Study on Mitigating Factors

Effect of dose‐rate and dose fractionation on radiation‐induced hemolysis of human erythrocytes

Sterilization by Gamma Irradiation

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