Contributions of PIP2-specific-phospholipase C and free salicylic acid to heat acclimation-induced thermotolerance in pea leaves

The plasma membrane H+-ATPase is related to the development of salicylic acid-induced thermotolerance in pea leaves

“…1a), which was similar to the results obtained by Dat et al (1998) and Liu et al (2006a). The content of SAG, a major conjugated form of SA, remained stable up to 60 min post-heat treatment (Fig.…”

“…The plasma membrane vesicles were prepared according to the methods of Faraday and Spanswick (1992) and Liu et al (2006a). All steps were carried out at 4°C.…”

“…MDA content was measured as described by Liu et al (2006a) and Pan et al (2006). The pea leaves (2 g) were homogenized in 5 ml of 0.1% trichloroacetic acid (TCA) and centrifuged at 10,0009g for 30 min.…”

“…To investigate whether heat acclimation induces the development of thermotolerance and whether PM H ? -ATPase is involved in heat signaling, we performed the MDA assay on pea leaves that were heat stressed at 48 ± 0.5°C after the pretreatments with water or the chemicals under 38 ± 0.5°C for 3 h. The previous studies Liu et al 2006a) had shown that similar thermotolerance could be developed between the treatment of endogenous SA at 25 ± 0.5°C and the treatment of heat acclimation at 38 ± 0.5°C. That is, SAtreatment is similar to heat-acclimated treatment.…”

Signal mediators at induction of heat resistance of wheat plantlets by short-term heating

“…On the other hand, the effect of the increase of H 2 O 2 content was also levelled by neomycin influen ce. This compound, binding phosphatidylinositol biphosphates, can inhibit the phosphatidylinositol-specific phospholipase C [22] and thus prevent the accumulation of reaction product inozitol-1,4,5-phosphate (IP 3 ), which influen ces calcium influx in the cytosol from endocellular compartments [23].…”