Contributions of Electron Microscopy to Fungal Classification

Cole, Garry T.

doi:10.1093/icb/19.2.589

Cited by 8 publications

(3 citation statements)

References 119 publications

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“…Morphological intergradations between these two basic types of septa are recognized (262,281,405), as well as certain artifacts of dolipore septal ultrastructure (213), and the phylogenetic implications of such characters are uncertain (201). Nevertheless, identification of septal morphology has contributed significantly to considerations of whether a particular conidial fungus has an ascomycetous or basidiomycetous affinity (77). Occurrence of mainly simple, uniperforate septa of the type shown in Fig.…”

Section: Septation and Secessionmentioning

confidence: 99%

Models of cell differentiation in conidial fungi.

Cole¹

1986

Microbiological Reviews

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Section: Septation and Secessionmentioning

confidence: 99%

Models of cell differentiation in conidial fungi.

Cole¹

1986

Microbiological Reviews

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“…The ultrastructural features of sexual spores are considered to be very important in the delimitation of taxa for major groups of fungi (Cole 1979). SEM imaging of basidiospore surfaces has been used for taxonomic studies on some basidiomycetes, whose basidiospores are heavily ornamented.…”

Section: Introductionmentioning

confidence: 99%

Use of ionic liquid in fungal taxonomic study of ultrastructure of basidiospore ornamentation

et al. 2011

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An ionic liquid (IL) is a kind of salt that stays in a molten state even at room temperature. Since ILs do not vaporize even under vacuum conditions and show high ionic conductivity, they can be used in scanning electron microscopy (SEM) studies. The ultrastructural features of basidiospore ornamentation are considered to be important in the delimitation of taxa for fungi. In the present study, we carried out SEM observations on basidiospores that were subjected to an IL treatment, and evaluated the usefulness of this method in comparison with a conventional preparation method in which dehydrating, drying and platinum (Pt) coating were used. Using the conventional method, a considerable number of basidiospores was lost from the gill tissues; however, using the IL method, the decrease in basidiospores was extremely small. No significant differences in ultrastructural morphology or basidiospore size were found between Pt-coated basidiospores and IL-treated ones. SEM images of Pt-coated basidiospores tended to have higher contrast than those of IL-treated ones. Charging effects were observed with Pt-coated basidiospores, especially at the tips of the ornaments, whereas no such effects occurred for the IL-treated ones. In addition, small crinkles were observed in the Pt-coated basidiospores, but not in the IL-treated ones. These results suggest that the IL method is useful for fungal taxonomic studies.

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“…The scanning electron microscope (SEM) has broad applications to fungal research but has been particularly valuable for taxonomic and developmental studies of microfungi (Moore and Grand, 1970;Cole and Behnke, 1975;Cole, 1976Cole, , 19790' Donnell, 1979). As in other biological disciplines, SEM images at the outset generated widespread aesthetic appeal, as well as skepticism among investigators "who saw little application in a device the main function of which was to produce snapshots of surfaces" (Heslop-Harrison, 1979).…”

Section: Introductionmentioning

confidence: 99%