Contributions of capillary pathway size and neutrophil deformability to neutrophil transit through rabbit lungs

Wiggs, Barry; English, D.; Quinlan, William M.; Doyle, Nicholas A.; Hogg, J. C.; Doerschuk, Claire M.

doi:10.1152/jappl.1994.77.1.463

Cited by 90 publications

(76 citation statements)

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“…In addition to vascular adhesion molecule interaction with stem cells, cell type-specifi c deformability might also be involved in pulmonary stem cell passage. Although our data do not allow us to further speculate on this issue, Wiggs et al demonstrated the impact of capillary pathway size and neutrophil deformability on neutrophil transit through rabbit lungs [16]. Therefore, the groups demonstrating the majority of infused stem cells to be trapped inside the pulmonary system.…”

Section: Resultsmentioning

confidence: 61%

Pulmonary Passage is a Major Obstacle for Intravenous Stem Cell Delivery: The Pulmonary First-Pass Effect

Fischer¹,

Harting²,

Jiménez³

et al. 2009

Stem Cells and Development

1,061

830

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Section: Resultsmentioning

confidence: 61%

Pulmonary Passage is a Major Obstacle for Intravenous Stem Cell Delivery: The Pulmonary First-Pass Effect

Fischer¹,

Harting²,

Jiménez³

et al. 2009

Stem Cells and Development

1,061

830

View full text Add to dashboard Cite

“…At branch points, this celldepleted wall region allows a progressively smaller fraction of cells to enter the daughter vessel than the parent vessel, causing a progressive reduction in hematocrit as blood courses its way from arterioles to capillaries (55). However, in contrast to RBCs, the neutrophil concentrations in capillaries are up to 40 -80 times higher than in larger blood vessels (56,57). Although some differences can be explained by the longer neutrophil transit times through capillaries, chemokine gradients may promote neutrophil circulation via narrow capillaries instead of the larger "thoroughfare" arteriolovenular shunts (58).…”

Section: Discussionmentioning

confidence: 99%

Low Intensity Shear Stress Increases Endothelial ELR+ CXC Chemokine Production via a Focal Adhesion Kinase-p38β MAPK-NF-κB Pathway

Shaik

Soltau

Chaturvedi

et al. 2009

Journal of Biological Chemistry

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CXC chemokines with a glutamate-leucine-arginine (ELR) tripeptide motif (ELR؉ CXC chemokines) play an important role in leukocyte trafficking into the tissues. For reasons that are not well elucidated, circulating leukocytes are recruited into the tissues mainly in small vessels such as capillaries and venules. Because ELR ؉ CXC chemokines are important mediators of endothelial-leukocyte interaction, we compared chemokine expression by microvascular and aortic endothelium to investigate whether differences in chemokine expression by various endothelial types could, at least partially, explain the microvascular localization of endothelial-leukocyte interaction. Both in vitro and in vivo models indicate that ELR ؉ CXC chemokine expression is higher in microvascular endothelium than in aortic endothelial cells. These differences can be explained on the basis of the preferential activation of endothelial chemokine production by low intensity shear stress. Low shear activated endothelial ELR ؉ CXC chemokine production via cell surface heparan sulfates, ␤ 3 -integrins, focal adhesion kinase, the mitogen-activated protein kinase p38␤, mitogen-and stress-associated protein kinase-1, and the transcription factor.

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“…Studies in humans undergoing cardiac catheterization show that alveolar compression results in an immediate arteriovenous difference of PMN across the lung that reverses when alveolar pressure is returned to normal (21). Mechanical deformation of PMN has been demonstrated in pulmonary microvessels (5), and Wiggs et al (29) have shown that PMN of ,7 µm in diameter negotiate the capillary bed in the same way as 4-µm nondeformable beads. These findings indicate that the PMN deform in the pulmonary capillary bed, but whether this deformation is an active or passive process remains to be determined.…”

mentioning

confidence: 99%

Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes

Kitagawa

Eeden

Redenbach

et al. 1997

Journal of Applied Physiology

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. Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes. J. Appl. Physiol. 82(5): 1397-1405, 1997.-The present studies were designed to test the hypothesis that mechanical deformation of polymorphonuclear leukocytes (PMN) leads to functional changes that might influence their transit in the pulmonary capillaries. Human leukocytes were passed through 5-or 3-µm-pore polycarbonate filters under controlled conditions. Morphometric analysis showed that the majority of PMN were deformed and that this deformation persisted longer after filtration through 3-µm filters than through 5-µm filters (P , 0.05) but did not result in the cytoskeletal polarization characteristic of migrating cells. Flow cytometric studies of the filtered PMN showed that there was a transient increase in the cytosolic free Ca 21 concentration after both 3-and 5-µm filtration (P , 0.01) with an increase in F-actin content after 3-µm filtration (P , 0.05). Although L-selectin expression on PMN was not changed by either 5-or 3-µm filtration, CD18 and CD11b were increased by 3-µm filtration (P , 0.05). Priming of the PMN with N-formyl-methionyl-leucyl-phenylalanine (0.5 nM) before filtration resulted in an increase of CD11b by both 5 (P , 0.05)-and 3-µm (P , 0.01) filtration. Neither 5-nor 3-µm filtration induced hydrogen peroxide production. We conclude that mechanical deformation of PMN, similar to what occurs in the pulmonary microvessels, induces both structural and functional changes in the cells, which might influence their passage through the pulmonary capillary bed. neutrophils; deformability; F-actin; adhesion molecules THE PULMONARY MICROVESSELS restrict the passage of polymorphonuclear leukocytes (PMN) because of the discrepancy between the size of the PMN and the size of the lung capillary segments (5, 14, 15). Although erythrocytes [red blood cells (RBC)] have similar maximum dimensions to PMN, their greater deformability allows them to negotiate these restrictions more quickly (14). This results in pulmonary capillary transit times that are 60-100 times longer for PMN than for RBC, and this concentrates PMN with respect to RBC in pulmonary capillaries (14,15).Stimuli such as peptide chemoattractants, endotoxin, and smoking are known to decrease PMN deformability and further increase the concentration of PMN in pulmonary capillaries (6,18,27,30). Several in vitro filtration studies have measured the biophysical properties of PMN, particularly their size and deformability, and have shown that these factors determine the magnitude of PMN sequestration in the pulmonary microvessels (6, 18, 27, 30). The importance of the discrepancy between PMN and pulmonary capillary dimensions in causing PMN sequestration in the lung has also been demonstrated during forced expiratory maneuvers in which raised intra-alveolar pressure compresses alveolar microvessels and delays PMN (21). Studies in humans undergoing cardiac catheterization show that alveolar compression results in an immediate arteriovenous difference o...

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Contributions of capillary pathway size and neutrophil deformability to neutrophil transit through rabbit lungs

Cited by 90 publications

References 0 publications

Pulmonary Passage is a Major Obstacle for Intravenous Stem Cell Delivery: The Pulmonary First-Pass Effect

Pulmonary Passage is a Major Obstacle for Intravenous Stem Cell Delivery: The Pulmonary First-Pass Effect

Low Intensity Shear Stress Increases Endothelial ELR+ CXC Chemokine Production via a Focal Adhesion Kinase-p38β MAPK-NF-κB Pathway

Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes

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