Contribution to the Understanding of Retention of Animal Cells in Spinfilters

Trocha, M.; Rohr, Rudolf von; Sümeghy, Z.

doi:10.1007/978-94-011-5404-8_64

Cited by 4 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increases in rotation rate, however, cannot be applied indiscriminately in case of mammalian cell retention, due to the shear sensitivity of animal cells and also because above certain rotation rates clogging of the internal mesh surface has been shown to be enhanced (Trocha et al 1997).…”

Section: Introductionmentioning

confidence: 99%

CFD simulation of an internal spin-filter: evidence of lateral migration and exchange flow through the mesh

et al. 2009

View full text Add to dashboard Cite

In the present work Computational Fluid Dynamics (CFD) was used to study the flow field and particle dynamics in an internal spin-filter (SF) bioreactor system. Evidence of a radial exchange flow through the filter mesh was detected, with a magnitude up to 130-fold higher than the perfusion flow, thus significantly contributing to radial drag. The exchange flow magnitude was significantly influenced by the filter rotation rate, but not by the perfusion flow, within the ranges evaluated. Previous reports had only given indirect evidences of this exchange flow phenomenon in spin-filters, but the current simulations were able to quantify and explain it. Flow pattern inside the spinfilter bioreactor resembled a typical Taylor-Couette flow, with vortices being formed in the annular gap and eventually penetrating the internal volume of the filter, thus being the probable reason for the significant exchange flow observed. The simulations also showed that cells become depleted in the vicinity of the mesh due to lateral particle migration. Cell concentration near the filter was approximately 50% of the bulk concentration, explaining why cell separation achieved in SFs is not solely due to size exclusion. The results presented indicate the power of CFD techniques to study and better understand spin-filter systems, aiming at the establishment of effective design, operation and scale-up criteria.

show abstract

Section: Introductionmentioning

confidence: 99%

CFD simulation of an internal spin-filter: evidence of lateral migration and exchange flow through the mesh

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Because of its magnitude, which can be 10–100‐fold higher than the perfusion flux as observed in the present work, the exchange flow can be the main factor affecting the radial drag on cells. In addition, its outward component could help to explain the internal filter clogging with cells that has been experimentally observed in these devices . As discussed by Yabannavar et al for internal spin‐filters, exchange flow is likely to play a relevant role at early stages of cultivation when filter screen is less clogged with cells and thus more permeable.…”

Section: Resultsmentioning

confidence: 91%

“…In addition, its outward component could help to explain the internal filter clogging with cells that has been experimentally observed in these devices. 27 As discussed by Yabannavar et al 11 for internal spin-filters, exchange flow is likely to play a relevant role at early stages of cultivation when filter screen is less clogged with cells and thus more permeable.…”

Section: Flow Pattern In the Rcfmentioning

confidence: 98%

Rotating cylindrical filters used in perfusion cultures: CFD simulations and experiments

Figueredo‐Cardero

Martínez

Chico

et al. 2014

Biotechnology Progress

View full text Add to dashboard Cite

The particle and fluid dynamics in a rotating cylindrical filtration (RCF) system used for animal cell retention in perfusion processes was studied. A validated CFD model was used and the results gave numerical evidence of phenomena that had been earlier claimed, but not proven for this kind of application under turbulent and high mesh permeability conditions, such as bidirectional radial exchange flow (EF) through the filter mesh and particle (cells) lateral migration. Taylor vortices were shown to cause EF 10-100 times higher than perfusion flow, indicating that EF is the main drag source, at least in early stages of RCF operation. Particle lateral migration caused a cell concentration reduction (CCR) near the filter surface of approximately 10%, contributing significantly to cell separation in RCF systems and giving evidence that the mesh sieving effect is not the sole phenomenon underlying cell retention in RCF systems. Filter rotation rate was shown to significantly affect both EF and CCR. A higher separation efficiency (measured experimentally at 2,000-L bioreactor scale) and an enhanced CCR (predicted by the numerical simulations) were found for the same rotation rate range, indicating that there is an optimal operational space with practical consequences on RCF performance. Experimental data of a large-scale perfusion run employing the simulated RCF showed high cell viabilities for over 100 days, which is probably related to the fact that the computed shear stress level in the system was shown to be relatively low (below 20 Pa under all tested conditions).

show abstract

Effect of Different Variables on the Long-Term Spinfilter Clogging during Pilot-Scale Animal Cell Perfusion Runs

et al.

View full text Add to dashboard Cite

Contribution to the Understanding of Retention of Animal Cells in Spinfilters

Cited by 4 publications

References 3 publications

CFD simulation of an internal spin-filter: evidence of lateral migration and exchange flow through the mesh

CFD simulation of an internal spin-filter: evidence of lateral migration and exchange flow through the mesh

Rotating cylindrical filters used in perfusion cultures: CFD simulations and experiments

Effect of Different Variables on the Long-Term Spinfilter Clogging during Pilot-Scale Animal Cell Perfusion Runs

Contact Info

Product

Resources

About