Contribution to the study of micropacked columns in gas chromatography

Reglero, Guillermo; Herráiz, Marta; Cabezudo, M. D.; Fernández-Sánchez, Elena; García-Domínguez, José Antonio

doi:10.1016/s0021-9673(01)92471-9

Cited by 26 publications

(9 citation statements)

References 12 publications

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“…According to our previous experience on SFC of fat-soluble vitamins, the use of Carbowax 20M as stationary phase seemed to provide an acceptable selectivity, although vitamins E, K1, A, and D2 were only resolved in two peaks (E + K1 and A + Dz) (Ibhfiez et al, 1995a). Micropacked columns were made from a pretreated stainless steel tube (Chrompack) (20 cm x 0.5 mm) and a spherical porous siliceous support from Merck (Volaspher A-2, 100-125 pm) according to a previously reported procedure (Reglero et al, 1985). For this reason, in this work the combination of two different stationary phases was evaluated to try to improve the selectivity adjustment.…”

Section: Methodsmentioning

confidence: 99%

Optimization of Separation of Fat-Soluble Vitamins by Supercritical Fluid Chromatography Using Serial Micropacked Columns

Ibáñez¹,

Tabera²,

Reglero³

et al. 1995

J. Agric. Food Chem.

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The advantages of using two on-line coupled micropacked columns in supercritical fluid chromatography for analyzing fat-soluble vitamins are evaluated. A rotatable central composite experimental design is used to optimize the combination of some experimental variables (temperature gradient and pressure gradient) involved in the chromatographic separation. Relative standard deviations obtained under the conditions of the experimental design giving the highest response are also included. The analysis of fat-soluble vitamins occurring in real-life samples (a pharmaceutical preparation for newborns and an infant formula) is presented. . E.I. thanks the Ministerio de Educaci6n y Ciencia for a grant. JF940588R@ Abstract published in Advance ACS Abstracts, September 15, 1995.

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Section: Methodsmentioning

confidence: 99%

Optimization of Separation of Fat-Soluble Vitamins by Supercritical Fluid Chromatography Using Serial Micropacked Columns

Ibáñez¹,

Tabera²,

Reglero³

et al. 1995

J. Agric. Food Chem.

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“…The columns were prepared according to a previously reported procedure developed in our laboratory [27]. All columns were made from pretreated stainless steel tubes (Chrompack, Middelburg, The Netherlands; 0.5 m x 500 pm i.d.)…”

Section: Methodsmentioning

confidence: 99%

“…Typical dp/dc values range between 0.1 and 0.3. Several advantages in GC of this second type of column have been already emphasized [27]. The column may exhibit noticeable specific efficiencies at a low phase ratio (@ = V,/V,)…”

Section: Introductionmentioning

confidence: 99%

Large particle micropacked columns in supercritical fluid chromatography

Ibáñez

Alvarez

Reglero

et al. 1993

J Microcolumn Separations

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The advantages of using micropacked columns with low packing density and liquid stationary phases, which are currently used in gas chromatography, in supercritical fluid chromatography (SFC) have been studied. An equation fitted to 199 experimental data is proposed to describe the relationship between the plate height and the linear mobile phase velocity of the mobile phase in a micropacked column. A comparison of the perfonnances of micropacked, open capillary, and standard packed columns in SFC is presented. The pressure drop at the optimal linear velocity per unit column length and per theoretical plate were evaluated and considered comparatively. Chromatographic efficiency, resolution, speed of analysis, and separation impedance achievable with each column were also evaluated. Preliminary results with column pressure drops similar to those of open tubular columns look promising. Data obtained lead to the conclusion that the use of large particle micropacked columns in SFC could be a suitable approach for the analysis of samples of medium complexity, where trace compound detection may require the use of columns having high sample capacities as well as high specific efficiencies.

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“…A polarity test Micropacked column, which have internal diameters less than 1 mm and a packing density similar to the columns described above (see section 1.7.1), are packed by special methods, since they are generally used in longer lengths and operated at higher inlet pressures than conventional packed columns [128,129]. A polarity test Micropacked column, which have internal diameters less than 1 mm and a packing density similar to the columns described above (see section 1.7.1), are packed by special methods, since they are generally used in longer lengths and operated at higher inlet pressures than conventional packed columns [128,129].…”

Section: Column Preparation and Evaluationmentioning

confidence: 99%

The Column in Gas Chromatography

Poole

1991

Chromatography Today

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Contribution to the study of micropacked columns in gas chromatography

Cited by 26 publications

References 12 publications

Optimization of Separation of Fat-Soluble Vitamins by Supercritical Fluid Chromatography Using Serial Micropacked Columns

Optimization of Separation of Fat-Soluble Vitamins by Supercritical Fluid Chromatography Using Serial Micropacked Columns

Large particle micropacked columns in supercritical fluid chromatography

The Column in Gas Chromatography

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