Contribution to the interaction of antamanide with the trivalent terbium cation

Böhm, Stanislav; Makrlík, Emanuel; Vaňura, Petr

doi:10.1007/s00706-014-1340-4

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…With such vast functionality, metal‐binding proteins and peptides are subjects of interest both in fundamental and in applied research. Univalent, divalent, and trivalent metal cations are known to form complexes with AA, e.g., complexes of AA with Li + , K + , Ca 2+ , and Tb 3+ ions were investigated by experimental as well as theoretical methods. Circular dichroism spectroscopy and conductivity measurements were applied to study binding of AA with Mg 2+ and Ca 2+ ions.…”

Section: Introductionmentioning

confidence: 99%

Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Pangavhane

Makrlík

Ruzza³

et al. 2019

Electrophoresis

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Affinity capillary electrophoresis (ACE) and pressure‐assisted ACE were employed to study the noncovalent molecular interactions of antamanide (AA), cyclic decapeptide from the deadly poisonous fungus Amanita phalloides, with univalent (Li+, Na+, K+, and NH4+) and divalent (Mg2+ and Ca2+) cations in methanol. The strength of these interactions was quantified by the apparent stability constants of the appropriate AA‐cation complexes. The stability constants were calculated using the nonlinear regression analysis of the dependence of the effective electrophoretic mobility of AA on the concentration of the above ions in the BGE (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pHMeOH 7.8, containing 0–50 mM concentrations of the above ions added in the form of chlorides). Prior to stability constant calculation, the AA effective mobilities measured at actual temperature inside the capillary and at variable ionic strength of the BGEs were corrected to the values corresponding to the reference temperature of 25°C and to the constant ionic strength of 10 mM. From the above ions, sodium cation interacted with AA moderately strong with the stability constant 362 ± 16 L/mol. K+, Mg2+, and Ca2+ cations formed with AA weak complexes with stability constants in the range 37–31 L/mol decreasing in the order K+ > Ca2+ > Mg2+. No interactions were observed between AA and small Li+ and large NH4+ cations.

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Section: Introductionmentioning

confidence: 99%

Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Pangavhane

Makrlík

Ruzza³

et al. 2019

Electrophoresis

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“…B, form complexes with a variety of metal cations . Complexes of AA with several metal cations, for example, K + , Ba 2+ , and Tb 3+ ions, were investigated by both experimental and theoretical methods . Binding of AA to Mg 2+ and Ca 2+ ions was studied by the circular dichroism spectroscopy and by conductivity measurements .…”

Section: Introductionmentioning

confidence: 99%

Affinity capillary electrophoresis and density functional theory study of noncovalent interactions of cyclic peptide [Gly⁶]‐antamanide with small cations

et al. 2017

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ACE and density functional theory were employed to study the noncovalent interactions of cyclic decapeptide glycine-6-antamanide ([Gly ]AA), synthetic derivative of native antamanide (AA) peptide from the deadly poisonous fungus Amanita phalloides, with small cations (Li , Rb , Cs , NH , and Ca ) in methanol. The strength of these interactions was quantified by the apparent stability constants of the appropriate complexes determined by ACE. The stability constants were calculated using the nonlinear regression analysis of the dependence of the effective electrophoretic mobility of [Gly ]AA on the concentration of the above ions in the BGE (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pH 7.8, containing 0-70 mM concentrations of the above ions added in the form of chlorides). Prior to stability constant calculation, the effective mobilities measured at actual temperature inside the capillary and at variable ionic strength of the BGEs were corrected to the values corresponding to the reference temperature of 25°C and to the constant ionic strength of 10 mM. From the above ions, Rb and Cs cations interacted weakly with [Gly ]AA but no interactions of [Gly ]AA with univalent Li and NH ions and divalent Ca ion were observed. The apparent stability constants of [Gly ]AA-Rb and [Gly ]AA-Cs complexes were found to be equal to 13 ± 4 and 22 ± 3 L/mol, respectively. The structural characteristics of these complexes, such as position of the Rb and Cs ions in the cavity of the [Gly ]AA molecule and the interatomic distances within these complexes, were obtained by the density functional theory calculations.

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Affinity capillary electrophoresis and quantum mechanical calculations applied to investigation of [Gly⁶]‐antamanide binding with sodium and potassium ions

et al. 2017

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ACE in a free solution and quantum mechanical density functional theory have been applied to the investigation of interactions of glycine-6-antamanide ([Gly ]AA), a synthetic derivative of cyclic decapeptide antamanide isolated from the highly poisonous mushroom Amanita phalloides, with sodium or potassium ions in methanol. First, from the dependence of effective electrophoretic mobility of [Gly ]AA on Na or K ions concentration in the BGE (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pH 7.8, containing 0-50 mM NaCl or 0-40 mM KCl), the apparent binding (stability) constants of [Gly ]AA-Na and [Gly ]AA-K complexes were evaluated as 26 ± 1 and 14 ± 1 L/mol, respectively. The employed ACE method included correction of the effective mobilities measured at ambient temperature and at variable ionic strength of the BGEs to the mobilities related to the reference temperature 25°C and to the constant ionic strength 10 mM. Second, the interaction energies of the [Gly ]AA-Na and [Gly ]AA-K complexes (-466.3 and -345.2 kJ/mol, respectively) and the structural details of these complexes, such as position of the Na and K ions in the cavity of the [Gly ]AA molecule and the interatomic distances within these complexes, were determined by the density functional theory calculations.

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Contribution to the interaction of antamanide with the trivalent terbium cation

Cited by 3 publications

References 25 publications

Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Affinity capillary electrophoresis and density functional theory study of noncovalent interactions of cyclic peptide [Gly⁶]‐antamanide with small cations

Affinity capillary electrophoresis and quantum mechanical calculations applied to investigation of [Gly⁶]‐antamanide binding with sodium and potassium ions

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Contribution to the interaction of antamanide with the trivalent terbium cation

Cited by 3 publications

References 25 publications

Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Affinity capillary electrophoresis and density functional theory study of noncovalent interactions of cyclic peptide [Gly6]‐antamanide with small cations

Affinity capillary electrophoresis and quantum mechanical calculations applied to investigation of [Gly6]‐antamanide binding with sodium and potassium ions

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Affinity capillary electrophoresis and density functional theory study of noncovalent interactions of cyclic peptide [Gly⁶]‐antamanide with small cations

Affinity capillary electrophoresis and quantum mechanical calculations applied to investigation of [Gly⁶]‐antamanide binding with sodium and potassium ions