Contribution to Apricot Genetic Analysis With Rflp, Rapd and Aflp Markers

Hurtado, Maria; Badenes, M. L.; Llácer, G.; Westman, A.; Beck, Erwin; Abbott, G. A.

doi:10.17660/actahortic.2001.546.54

Cited by 11 publications

(9 citation statements)

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“…In this study, the cladograms derived from AFLP and SNPs clustered differently, which might be due to the difference between AFLP and SNP markers. This difference in clustering is common in other reports, demonstrating that the relationship obtained using 1 genetic marker might not fully agree with that obtained using a different genetic marker (Roldán-Ruiz et al 2001;King and Burke 1999;Hurtado et al 2001). This could result from the differences in the genetic markers within the targeted genome, the mutation rate, or the dominant (AFLP) or codominant (SNP) nature of the marker systems.…”

Section: Discussioncontrasting

confidence: 52%

Genetic relationships among fruiting-mei (Prunus mume Sieb. et Zucc.) cultivars evaluated with AFLP and SNP markers

Fang

Twito

Zhang

et al. 2006

Genome

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Genetic relationships among 50 fruiting-mei (Prunus mume Sieb. et Zucc.) cultivars from China and Japan were investigated, using 767 amplified fragment length polymorphism (AFLP) and 103 single nucleotide polymorphism (SNP) markers. The polymorphism among the cultivars was found to be 69.77%, based on EcoR I + Mse I AFLP primer pairs. The sequence alignment of 11 group sequences, derived from 50 samples, yielded 103 SNPs; the total length of genomic sequences was 3683 bp. Among these SNPs, 73 were heterozygous in the loci of different cultivars. The SNP distribution was 58% transition, 40% transversion, and 2% InDels. There was also 1 trinucleotide deletion. AFLP and SNP markers allowed us to evaluate the genetic diversity of these 50 fruiting-mei cultivars. The 2 derived cladograms did display some differences: all cultivars formed 2 subclusters (1A and 1B) in the cladogram based on AFLP polymorphisms, and formed 3 subclusters (2A, 2B, and 2C) in the cladogram based on SNP polymorphisms; and, in the cladogram based on AFLP polymorphisms, most cultivars from the Guangdong to Fujian provinces (G-F) in China, from the Yunnan, Hunan, and Sichuan provinces (Y-S-H) in China, and from Japan grouped in cluster 1A, and 18 (78.26%) of 23 cultivars from Jiangsu to Zhejiang provinces in China (J-Z) grouped in cluster 1B. The results demonstrate that mei cultivars from Japan are clustered with cultivars from China, and support the hypothesis that mei in Japan were introduced from China. Cultivars from the J-Z region of China have more genetic similarities. Cultivars from the G-F and Y-S-H regions have fewer genetic similarities and suggest more germplasm exchanges in the past.

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Section: Discussioncontrasting

confidence: 52%

Genetic relationships among fruiting-mei (Prunus mume Sieb. et Zucc.) cultivars evaluated with AFLP and SNP markers

Fang

Twito

Zhang

et al. 2006

Genome

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“…Studies of other plants found the similar results between the genetic clustering and their geographic origins. Hurtado et al (2001) used the restriction fragment length polymorphism (RFLP), random amplifi ed polymorphism DNA (RAPD), and AFLP markers to separate sixteen apricot cultivars into different clusters and the clustering were very similar to their known geographic origins. Sanz-Cortés et al (2001) reported that there was a correlation between the clustering of olive cultivars based on genetic similarity from RAPD marker analysis and their geographic origins in the Valencia region of Spain.…”

Section: Discussionmentioning

confidence: 99%

Genotyping Fruiting Mei (Prunus mume Sieb. et Zucc.) Cultivars Using Amplified Fragment-length Polymorphism Markers

Fang¹,

Qiao²,

Zhang³

et al. 2005

HortSci

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We used amplified fragment length polymorphism (AFLP) markers to analyze 14 fruiting mei cultivars from China and Japan. The levels of polymorphism and genetic relationship among cultivars were studied using two types of AFLP primer combinations [EcoR I + Mse I (E+M) and EcoR I + Taq I (E+T)] and the combined data from both types of primer combinations (E+M+T). The polymorphism among the cultivars was 57.92% based on E+M primers and 63.04% based on E+T primers. All three dendrograms generated by the three sets of data showed similar relationships among the fruiting mei cultivars. The corresponding main clusters contained the same cultivars and the subgroups correlated closely with the known geographic origins of the cultivars.

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“…The number of loci assessed should be as high as possible and preferably should be distributed at random in the genome to ensure adequate genome coverage (Aranzana et al 2003a;2003b). Different type of molecular markers, from isoenzymes (Gašíc et al 2000;Messeguer et al 1987) to DNA markers like RAPDs, AFLPs, and RFLPs (Gogorcena and Parfitt 1994;Hurtado et al 2001;Quarta et al 2001) or SSR (Bouhadida et al 2009;Decroocq et al 2004) have been used to characterize genetic diversity in Prunus germplasm collections.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of introduced and local Spanish peach cultivars determined by SSR markers

Bouhadida

Sánchez

Gonzalo

et al. 2010

Tree Genetics & Genomes

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A set of 94 peach cultivars including Spanish native peach and foreign commercial cultivars were analyzed using 15 SSR markers, selected for their high level of polymorphism. The number of alleles obtained varied from two to 11 with an average of 6.73 giving 185 different genotypes. All the cultivars showed a unique genetic profile, each one using different genotypic combination of all loci. BPPCT001 was the most informative locus showing also the highest discrimination power. Only six loci allowed the unambiguous separation of all the Spanish native cultivars studied, and the genotypic combination of only eight loci permitted the total differentiation of the 94 peach cultivars analyzed. The six selected loci (BPPCT001, BPPCT006, BPPCT008, PS9f8, UDP98-022, and UDP98-412) seem to be very useful for future Spanish peach identification works, and they will help to establish a molecular data base for native peach cultivars. UPGMA analysis was performed from the genetic distance matrix, and allowed the arrangement of all genotypes according to their genetic diversity. The genetic diversity among cultivars, observed in this work, led to their separation according to their regional origin, their morphological characteristics, and especially according to their fruit traits. Analysis of molecular variance was performed for seven populations from different regions of Spain and USA to examine the distribution of genetic variation of the studied accessions, showing that the major variation occurred within populations in each geographic site. The results reveal the existence of two diversity regions in Spain for peach germplasm.

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Contribution to Apricot Genetic Analysis With Rflp, Rapd and Aflp Markers

Cited by 11 publications

References 0 publications

Genetic relationships among fruiting-mei (Prunus mume Sieb. et Zucc.) cultivars evaluated with AFLP and SNP markers

Genetic relationships among fruiting-mei (Prunus mume Sieb. et Zucc.) cultivars evaluated with AFLP and SNP markers

Genotyping Fruiting Mei (Prunus mume Sieb. et Zucc.) Cultivars Using Amplified Fragment-length Polymorphism Markers

Genetic variability of introduced and local Spanish peach cultivars determined by SSR markers

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