Contribution of white grubs (Scarabaeidae: Coleoptera) to N2O emissions from tropical soils

Majeed, Muhammad Zeeshan; Miambi, Edouard; Barois, Isabelle; Randriamanantsoa, Richard; Blanchart, Éric; Brauman, Alain

doi:10.1016/j.soilbio.2014.03.025

Cited by 13 publications

(19 citation statements)

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“…Moreover, the abundance of lipids within the pocket pole unveiled by Raman micro-spectroscopy ( Figure 6 ) makes possible that these compounds are used by the pocket symbionts as electron donors for respiratory processes using nitrate as an electron acceptor (NO 3 - ). Denitrification has already been reported in other rhizophagous white grubs ( Majeed and Miambi, 2014 ). The presence of pockets could be also related to the rhizophagous diet of the larvae, as they were spotted in the rhizophagous larvae of M. melolontha as well ( Figure 1F ), but no similar structure was found in Pachnoda marginata (Supplementary Figure S4 ), whose grub-like larvae thrive not on roots but on humic acids.…”

Section: Discussionmentioning

confidence: 53%

Bacterial Community and PHB-Accumulating Bacteria Associated with the Wall and Specialized Niches of the Hindgut of the Forest Cockchafer (Melolontha hippocastani)

et al. 2017

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A characterization of the bacterial community of the hindgut wall of two larval and the adult stages of the forest cockchafer (Melolontha hippocastani) was carried out using amplicon sequencing of the 16S rRNA gene fragment. We found that, in second-instar larvae, Caulobacteraceae and Pseudomonadaceae showed the highest relative abundances, while in third-instar larvae, the dominant families were Porphyromonadaceae and Bacteroidales-related. In adults, an increase of the relative abundance of Bacteroidetes, Proteobacteria (γ- and δ- classes) and the family Enterococcaceae (Firmicutes) was observed. This suggests that the composition of the hindgut wall community may depend on the insect’s life stage. Additionally, specialized bacterial niches hitherto very poorly described in the literature were spotted at both sides of the distal part of the hindgut chamber. We named these structures “pockets.” Amplicon sequencing of the 16S rRNA gene fragment revealed that the pockets contained a different bacterial community than the surrounding hindgut wall, dominated by Alcaligenaceae and Micrococcaceae-related families. Poly-β-hydroxybutyrate (PHB) accumulation in the pocket was suggested in isolated Achromobacter sp. by Nile Blue staining, and confirmed by gas chromatography–mass spectrometry analysis (GC-MS) on cultured bacterial mass and whole pocket tissue. Raman micro-spectroscopy allowed to visualize the spatial distribution of PHB accumulating bacteria within the pocket tissue. The presence of this polymer might play a role in the colonization of these specialized niches.

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Section: Discussionmentioning

confidence: 53%

Bacterial Community and PHB-Accumulating Bacteria Associated with the Wall and Specialized Niches of the Hindgut of the Forest Cockchafer (Melolontha hippocastani)

et al. 2017

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“…There are also a few studies available from other soil faunal groups, e.g. ants [ 54 ] and one laboratory-based study on Scarabaeidae larvae [ 55 ], but earthworms are the only faunal group for which a considerable amount of literature on soil N 2 O emissions is available [ 9 ]. Earthworms are not host to an endemic denitrifier community in their gut system, but in their presence N 2 O emissions can increase by more than 40% due to the activation of ingested nitrate- and nitrite-reducing soil bacteria during earthworm gut passage [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…Earthworms are not host to an endemic denitrifier community in their gut system, but in their presence N 2 O emissions can increase by more than 40% due to the activation of ingested nitrate- and nitrite-reducing soil bacteria during earthworm gut passage [ 14 ]. It is unclear if Scarabaeidae larvae are capable to affect soil N 2 O emissions in a similar manner, but the study by Majeed et al [ 55 ] points in the same direction, identifying denitrification as the most likely pathway for N 2 O production in larval guts. Whereas our data base was too inconsistent for upscaling, Majeed et al [ 55 ] attributed 0.2–1.8% of N 2 O emissions from tropical soils to Scarabaeidae larvae.…”

Section: Discussionmentioning

confidence: 99%

“…It is unclear if Scarabaeidae larvae are capable to affect soil N 2 O emissions in a similar manner, but the study by Majeed et al [ 55 ] points in the same direction, identifying denitrification as the most likely pathway for N 2 O production in larval guts. Whereas our data base was too inconsistent for upscaling, Majeed et al [ 55 ] attributed 0.2–1.8% of N 2 O emissions from tropical soils to Scarabaeidae larvae. In contrast to our study, they observed consistent N 2 O emissions from their laboratory-incubated larvae with an average emission rate of 10 ng N 2 O g -1 larval fresh weight h -1 .…”

Section: Discussionmentioning

confidence: 99%

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First field estimation of greenhouse gas release from European soil-dwelling Scarabaeidae larvae targeting the genus Melolontha

Görres

Kammann

2020

PLoS ONE

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Arthropods are a major soil fauna group, and have the potential to substantially influence the spatial and temporal variability of soil greenhouse gas (GHG) sinks and sources. The overall effect of soil-inhabiting arthropods on soil GHG fluxes still remains poorly quantified since the majority of the available data comes from laboratory experiments, is often controversial, and has been limited to a few species. The main objective of this study was to provide first insights into field-level carbon dioxide (CO 2 ), methane (CH 4 ), and nitrous oxide (N 2 O) release of soil-inhabiting larvae of the Scarabaeidae family. Larvae of the genus Melolontha were excavated at various sites in west-central and southern Germany, covering a wide range of different larval developmental stages, larval activity levels, and vegetation types. Excavated larvae were immediately incubated in the field to measure their GHG production. Gaseous carbon release of individual larvae showed a large inter- and intra-site variability which was strongly correlated to larval biomass. This correlation persisted when upscaling individual CO 2 and CH 4 production to the plot scale. Field release estimates for Melolontha spp. were subsequently upscaled to the European level to derive the first regional GHG release estimates for members of the Scarabaeidae family. Estimates ranged between 10.42 and 409.53 kt CO 2 yr -1 , and 0.01 and 1.36 kt CH 4 yr -1 . Larval N 2 O release was only sporadically observed and not upscaled. For one site, a comparison of field- and laboratory-based GHG production measurements was conducted to assess potential biases introduced by transferring Scarabaeidae larvae to artificial environments. Release strength and variability of captive larvae decreased significantly within two weeks and the correlation between larval biomass and gaseous carbon production disappeared, highlighting the importance of field measurements. Overall, our data show that Scarabaeidae larvae can be significant soil GHG sources and should not be neglected in soil GHG flux research.

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“…These animals significantly influence nutrient cycling processes (DE VRIES et al, 2013;LUBBERS et al, 2013;WAGG et al, 2014). Moreover, these animals also have an acknowledged role in building and stabilizing soil aggregates with direct implications on flood and erosion control (SIX et al, 2004;BROWN, 2007), and on soil carbon storage and net soil greenhouse-gas emissions (LUBBERS et al, 2013;MAJEED et al, 2014). Some species feed on litter and organic residues on the soil surface.…”

Section: Introductionmentioning

confidence: 99%

Soil engineering by macroinvertebrates: controls on soil organic matter storage across land use change

Franco¹

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Contribution of white grubs (Scarabaeidae: Coleoptera) to N2O emissions from tropical soils

Cited by 13 publications

References 48 publications

Bacterial Community and PHB-Accumulating Bacteria Associated with the Wall and Specialized Niches of the Hindgut of the Forest Cockchafer (Melolontha hippocastani)

Bacterial Community and PHB-Accumulating Bacteria Associated with the Wall and Specialized Niches of the Hindgut of the Forest Cockchafer (Melolontha hippocastani)

First field estimation of greenhouse gas release from European soil-dwelling Scarabaeidae larvae targeting the genus Melolontha

Soil engineering by macroinvertebrates: controls on soil organic matter storage across land use change

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