Abstract:Fermentation is extremely important for the formation of the special flavor of Wuyi rock tea. This study determined volatile metabolite contents using GC-MS technique and futher analyzed their odor characteristics during the traditional deep fermentation technology of Wuyi rock tea. The results showed that 17 characteristic compounds significantly changed during the first stage of the preliminary processing, namely fresh leaves, withering and fermentation. The key to the formation of floral aroma lied in dihyd… Show more
“…Aroma is an important index for assessing tea quality, and the level of tea aroma quality is closely related to the content of volatile compounds in tea [ 17 ]. There are many types of volatile compounds in tea, and different volatile compounds present different odor characteristics, while the intensity of odor characteristics of tea can be determined by judging the content of volatile compounds, which in turn evaluates the aroma intensity of tea [ 18 ]. Therefore, the determination of volatile compounds in tea is commonly used in the evaluation of tea aroma quality [ 19 ].…”
Aviation mutagenesis is a fast and efficient breeding method. In this study, we analyzed the effect of aviation mutagenesis on volatile compounds and odor characteristics in Dahongpao fresh leaves and gross tea for the first time. The results showed that aviation mutagenesis significantly increased the total volatile compounds of Dahongpao fresh leaves and gross tea. Aviation mutagenesis most critically significantly increased the content of beta-myrcene in Dahongpao fresh leaves, prompting its conversion to beta-pinene, cubebol, beta-phellandrene, zingiberene, (Z,Z)-3,6-nonadienal, and 6-pentyloxan-2-one after processing, which in turn enhanced the fruity, green, spicy, and woody odor characteristics of the gross tea. This study provided a reference for further exploration of aviation mutagenic breeding of Camellia sinensis.
“…Aroma is an important index for assessing tea quality, and the level of tea aroma quality is closely related to the content of volatile compounds in tea [ 17 ]. There are many types of volatile compounds in tea, and different volatile compounds present different odor characteristics, while the intensity of odor characteristics of tea can be determined by judging the content of volatile compounds, which in turn evaluates the aroma intensity of tea [ 18 ]. Therefore, the determination of volatile compounds in tea is commonly used in the evaluation of tea aroma quality [ 19 ].…”
Aviation mutagenesis is a fast and efficient breeding method. In this study, we analyzed the effect of aviation mutagenesis on volatile compounds and odor characteristics in Dahongpao fresh leaves and gross tea for the first time. The results showed that aviation mutagenesis significantly increased the total volatile compounds of Dahongpao fresh leaves and gross tea. Aviation mutagenesis most critically significantly increased the content of beta-myrcene in Dahongpao fresh leaves, prompting its conversion to beta-pinene, cubebol, beta-phellandrene, zingiberene, (Z,Z)-3,6-nonadienal, and 6-pentyloxan-2-one after processing, which in turn enhanced the fruity, green, spicy, and woody odor characteristics of the gross tea. This study provided a reference for further exploration of aviation mutagenic breeding of Camellia sinensis.
“…During the manufacturing process of RGT, the main volatile substances produced include alcohols, alkenes and esters, in which the content of alcohols gradually decreases, and the alkenes and esters show wave changes, which Foods 2024, 13, 1315 2 of 20 are obviously increased during green-making, and a large number of volatile compounds containing nitrogen are formed after baking [1,2]. RGT produces a large number of alcohols and aldehydes during fermentation, such as linalool, indole, nerol, (Z)-3-hexen-1-ol and (Z)-3-hexenyl acetate [3][4][5]. Other WRTs produce similar volatile substances, such as terpene, aldehyde and ester compounds, during the manufacturing process, and green-making has a significant effect on it, finally promoting the formation of flowery or fruity aroma characteristics [6][7][8].…”
Rougui Tea (RGT) is a typical Wuyi Rock Tea (WRT) that is favored by consumers for its rich taste and varied aroma. The aroma of RGT is greatly affected by the process of green-making, but its mechanism is not clear. Therefore, in this study, fresh leaves of RGT in spring were picked, and green-making (including shaking and spreading) and spreading (unshaken) were, respectively, applied after sun withering. Then, they were analyzed by GC-TOF-MS, which showed that the abundance of volatile compounds with flowery and fruity aromas, such as nerolidol, jasmine lactone, jasmone, indole, hexyl hexanoate, (E)-3-hexenyl butyrate and 1-hexyl acetate, in green-making leaves, was significantly higher than that in spreading leaves. Transcriptomic and proteomic studies showed that long-term mechanical injury and dehydration could activate the upregulated expression of genes related to the formation pathways of the aroma, but the regulation of protein expression was not completely consistent. Mechanical injury in the process of green-making was more conducive to the positive regulation of the allene oxide synthase (AOS) branch of the α-linolenic acid metabolism pathway, followed by the mevalonate (MVA) pathway of terpenoid backbone biosynthesis, thus promoting the synthesis of jasmonic acid derivatives and sesquiterpene products. Protein interaction analysis revealed that the key proteins of the synthesis pathway of jasmonic acid derivatives were acyl-CoA oxidase (ACX), enoyl-CoA hydratase (MFP2), OPC-8:0 CoA ligase 1 (OPCL1) and so on. This study provides a theoretical basis for the further explanation of the formation mechanism of the aroma substances in WRT during the manufacturing process.
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