Contribution of the secretory material of caecilian (amphibia: Gymnophiona) male Mullerian gland to motility of sperm: A study inUraeotyphlus narayani

George, Jancy M.; Mathew, Sheena; Kadalmani, Balamuthu; Girija, R.; Oommen, Oommen V.; Akbarsha, Mohammad A.

doi:10.1002/jmor.10300

Cited by 14 publications

(14 citation statements)

References 35 publications

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“…This hypothesis is supported by the fact that caecilian testicular spermatozoa are already motile, thus bypassing post‐testicular maturation (George et al . ).…”

Section: Discussionmentioning

confidence: 97%

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Histomorphological studies of the testis and male genital ducts of Supachai's caecilian, Ichthyophis supachaii Taylor, 1960 (Amphibia: Gymnophiona)

et al. 2014

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We investigated the structure of the male reproductive system in Ichthyophis supachaii. The testis comprises a series of mulberry-like lobes, each of which contains testis lobules occupied by germ cysts. A single cyst consists of synchronously developing germ cells. Six spermatogenic cell types, viz. primary spermatogonia, secondary spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa, have been identified and described. Notably, the testis of I. supachaii encompasses specific organization patterns of spermatids and spermatozoa during spermiogenesis. Spermiating cysts rupture and release spermatozoa to the collecting ducts, which are subsequently transported to the sperm duct, Wolffian duct and cloaca. We report for the first time ciliated cells in the epithelium of the caecilian Wolffian duct. The cloaca is divided into the urodeum and phallodeum. The urodeum has ciliated and glandular epithelia at its dorsolateral and ventral regions, respectively, as the lining of its internal surface. The muscular phallodeum is lined by ciliated epithelium. Paired Mullerian ducts lie parallel to the intestine and join the cloaca. The posterior portion of the duct is modified as the Mullerian gland. The most posterior region is non-glandular and lined by ciliated epithelium. Our findings contribute further to information on the reproductive biology of caecilians in Thailand.

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“…This hypothesis is supported by the fact that caecilian testicular spermatozoa are already motile, thus bypassing post‐testicular maturation (George et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…In the caecilian Wolffian duct, the ciliary activity from the ciliated cells may function to propel secretory products towards spermatozoa to support their motility (George et al . ).…”

Section: Discussionmentioning

confidence: 97%

Histomorphological studies of the testis and male genital ducts of Supachai's caecilian, Ichthyophis supachaii Taylor, 1960 (Amphibia: Gymnophiona)

et al. 2014

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“…The salamanders and newts have developed specialized sperm storage organs, spermathecae, located near to the cloaca. These are derived from the Mullerian duct, showing that this mode of sperm storage has evidently evolved independently of the oviduct (Wake and Dickie 1998; George et al. 2005).…”

Section: Evolutionary Aspects Of Sperm Storagementioning

confidence: 99%

Mechanisms of Sperm Storage in the Female Reproductive Tract: an Interspecies Comparison

Holt

2011

Reprod Domestic Animals

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Contents Once semen has been collected for artificial insemination, it is diluted into extenders designed to prevent its deterioration over the period prior to insemination. If the semen is not frozen, the extenders provide protection for a period of a few hours to a few days, depending on species. Despite the efforts of biotechnologists to increase the duration of storage without compromising fertility, there has been relatively little progress for many years. However, comparative studies in diverse species have revealed that long‐term sperm storage (up to months and years) within the female reproductive tract is relatively commonplace in reptiles, fishes, birds and amphibians. Even among mammals, some species of bat have evolved mechanisms for storing spermatozoa for several months in the uterus or oviduct so that they can mate in the autumn but postpone fertilization until the spring. We currently know little about the mechanisms that support such long‐term sperm storage, mainly because evidence from such species is either absent or fragmentary. Nevertheless, parallels between mammalian and other systems, where spermatozoa are sequestered in sperm storage tubules, suggest that the enclosure of spermatozoa within pockets of epithelial cells may be sufficient to achieve long‐term sperm storage. In addition, recent evidence from sperm‐storing bats has suggested an alternative, or additional, hypothesis that the modulation of apoptosis within epithelial cells is important in controlling sperm survival. Despite a lack of direct experimental evidence from a wide variety of species, I propose that there is now enough evidence to warrant investigation of these hypotheses.

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“…However, cryopreservation of testes macerates has limited applicability to wildlife conservation beyond terminal collections due to its invasive nature. For example, the sperm of several species of toad and caecilian possess a unique structure termed the mitochondrial vesicle, which is lacking in ranid frogs (George et al, 2005;Kouba, Vance, Frommeyer, & Roth, 2003). In doing so, animals are relatively unharmed during the process and multiple collections can be done on a single individual.…”

Section: Introductionmentioning

confidence: 99%

“…The lack of cross-species comparisons impedes the development of cryotechniques in amphibians because sperm cell differences (i.e., size and structural variations) can influence freezability. For example, the sperm of several species of toad and caecilian possess a unique structure termed the mitochondrial vesicle, which is lacking in ranid frogs (George et al, 2005;Kouba, Vance, Frommeyer, & Roth, 2003). This fragile structure facilitates sperm movement, but is often harmed in the cryopreservation process .…”

Section: Introductionmentioning

confidence: 99%

Applying cryopreservation to anuran conservation biology

Poo

Hinkson

2019

Conservat Sci and Prac

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Novel conservation methods have become increasingly important in protecting our remaining biodiversity in the face of unprecedented rates of species declines. One method of maintaining genetic and species diversity is through germplasm cryopreservation. However, our knowledge of cryopreservation relies heavily on studies in domestic or aquaculture species. Moreover, most studies are narrowly focused on a single species. Consequently, the broader application of these methods to wildlife conservation is often unknown or assumed. Here, we examine the response of four anuran species from two families (Anaxyrus fowleri, Anaxyrus baxteri, Lithobates pipiens, and Lithobates sevosus) to three sperm cryopreservation treatments (5, 10, and 15% N,N-dimethylformamide with 10% trehalose). Within each family, we selected a congeneric species pair with one common, nonthreatened species and one endangered species. We found that endangered species had significantly lower initial sperm quality, though recovery rate of sperm after cryopreservation was not significantly different between nonthreatened and endangered species. Overall, responses to the three treatments were consistent across species, with 5% DMFA with 10% trehalose producing the highest recovery rates in all four species. In addition, cryopreserved sperm were used to successfully produce hatchlings in both of the nonthreatened species. Our results demonstrate that standardized methods developed for anurans can be applied more widely across families and can be transferred from model species to species of conservation concern.These findings form a basis for further exploration into cryopreservation as an effective tool for wildlife conservation in amphibians. K E Y W O R D Samphibian, assisted reproductive technology, Bufonidae, cryobiology, Ranidae, sperm motility

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Contribution of the secretory material of caecilian (amphibia: Gymnophiona) male Mullerian gland to motility of sperm: A study inUraeotyphlus narayani

Cited by 14 publications

References 35 publications

Histomorphological studies of the testis and male genital ducts of Supachai's caecilian, Ichthyophis supachaii Taylor, 1960 (Amphibia: Gymnophiona)

Histomorphological studies of the testis and male genital ducts of Supachai's caecilian, Ichthyophis supachaii Taylor, 1960 (Amphibia: Gymnophiona)

Mechanisms of Sperm Storage in the Female Reproductive Tract: an Interspecies Comparison

Applying cryopreservation to anuran conservation biology

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