Contribution of the satellite encoded gene βC1 to cotton leaf curl disease symptoms

Qazi, Javaria; Amin, Imran; Mansoor, Shahid; Iqbal, Muhammad Javed; Briddon, R. W.

doi:10.1016/j.virusres.2007.04.002

Cited by 97 publications

(68 citation statements)

References 24 publications

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“…However, in both cases the symptoms suggested that the betasatellite was possibly responsible for enhancing symptoms. In the case of cowpea the plant exhibited enations which are typical of CLCuMB (Rouhibakhsh & Malathi, 2005;Qazi et al, 2007a). The plant identified in the present study exhibited some leaf crumpling which is not typical of MYMIV infection and may thus be due to TbLCB.…”

Section: Discussionmentioning

confidence: 53%

Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan

Ilyas

Qazi

Mansoor

et al. 2010

Journal of General Virology

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Grain legumes are an important source of dietary protein across southern Asia, but they suffer extensive losses due to several viruses that are members of the genus Begomovirus (family Geminiviridae), which are collectively known as legume yellow mosaic viruses (LYMVs). Despite their economic importance, little attention has been paid to LYMVs in Pakistan and only partial sequences of virus isolates originating from this country are available in the databases. Here, a survey of LYMVs occurring across Pakistan is described. Complete sequences of 44 components (23 DNA-A, 19 DNA-B and 2 betasatellites) were determined. The results show that only the mungbean yellow mosaic India virus (MYMIV) is of agricultural significance in Pakistan having been isolated from all cultivated grain legumes examined. Mungbean yellow mosaic virus, a significant crop pathogen in India, was only identified in a weed, which together with a novel species of LYMV we reported earlier, represents the first LYMV identified in non-cultivated plants. MYMIV was shown to occur as two types in Pakistan that show phylogeographical segregation. Additionally, two begomovirus species not considered pathogens of legumes and a betasatellite were isolated. This is of grave concern since it suggests that the presumed genetic isolation of the LYMVs in legumes may be being breached. LYMVs show little, if any, evidence of interspecific recombination with non-legume infecting begomoviruses. Thus, either recombination with non-legume viruses or interaction with betasatellites, which are host range and pathogenicity determining satellites of begomoviruses, could lead to the appearance of more aggressive virus variants/strains affecting legumes.

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Section: Discussionmentioning

confidence: 53%

Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan

Ilyas

Qazi

Mansoor

et al. 2010

Journal of General Virology

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“…The SCR contains a predicted hairpin structure that contains within the loop a nonanucleotide sequence (TA ATATTAC) that for geminiviruses marks the origin of virionstrand DNA replication. The ␤C1 gene is a pathogenicity determinant (27,28,33) and encodes all satellite functions identified so far, including suppression of RNA-mediated host defense (13) and possibly a role in virus movement (29). For many of the monopartite begomoviruses, the betasatellite is essential for inducing typical disease symptoms in the hosts from which they were isolated (6,9,30).…”

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confidence: 99%

Maintenance of an Old World Betasatellite by a New World Helper Begomovirus and Possible Rapid Adaptation of the Betasatellite

Nawaz-ul-Rehman

Mansoor

Briddon

et al. 2009

J Virol

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Begomoviruses (family Geminiviridae) cause major losses to crops throughout the tropical regions of the world. Begomoviruses originating from the New World (NW) and the Old World (OW) are genetically distinct. Whereas the majority of OW begomoviruses have monopartite genomes and whereas most of these associate with a class of symptom-modulating satellites (known as betasatellites), the genomes of NW begomoviruses are exclusively bipartite and do not associate with satellites. Here, we show for the first time that a betasatellite (cotton leaf curl Multan betasatellite [CLCuMuB]) associated with a serious disease of cotton across southern Asia is capable of interacting with a NW begomovirus. In the presence of CLCuMuB, the symptoms of the NW cabbage leaf curl virus (CbLCuV) are enhanced in Nicotiana benthamiana. However, CbLCuV was unable to interact with a second betasatellite, chili leaf curl betasatellite. Although CbLCuV can transreplicate CLCuMuB, satellite accumulation levels in plants were low. However, progeny CLCuMuB isolated after just one round of infection with CbLCuV contained numerous mutations. Reinoculation of one such progeny CLCuMuB with CbLCuV to N. benthamiana yielded infections with significantly higher satellite DNA levels. This suggests that betasatellites can rapidly adapt for efficient transreplication by a new helper begomovirus, including begomoviruses originating from the NW. Although the precise mechanism of transreplication of betasatellites by begomoviruses remains unknown, an analysis of betasatellite mutants suggests that the sequence(s) required for maintenance of CLCuMuB by one of its cognate begomoviruses (cotton leaf curl Rajasthan virus) differs from the sequences required for maintenance by CbLCuV. The significance of these findings and, particularly, the threat that betasatellites pose to agriculture in the NW, are discussed.

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“…This gene has the capacity to encode a 13-14-kDa protein comprising 118 amino acids, although some betasatellites have additional N-terminal amino acids [79,108]. Recently it has been shown that betasatellite to be pathogenicity determinant and suppressor of RNA silencing [16,66]. It also induced abnormal cell division in N. benthamiana [17].…”

Section: Betasatellitementioning

confidence: 99%

Mutational Analysis of Effectors Encoded by Monopartite Begomoviruses and Their Satellites

Shahid¹,

Sharma²,

Ikegami³

2012

Plant Science

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Contribution of the satellite encoded gene βC1 to cotton leaf curl disease symptoms

Cited by 97 publications

References 24 publications

Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan

Genetic diversity and phylogeography of begomoviruses infecting legumes in Pakistan

Maintenance of an Old World Betasatellite by a New World Helper Begomovirus and Possible Rapid Adaptation of the Betasatellite

Mutational Analysis of Effectors Encoded by Monopartite Begomoviruses and Their Satellites

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