Contribution of the patient microbiome to surgical site infection and antibiotic prophylaxis failure in spine surgery

Long, Dustin R.; Bryson-Cahn, Chloe; Waalkes, Adam; Holmes, Elizabeth A.; Penewit, Kelsi; Tavolaro, Celeste; Bellabarba, Carlo; Zhang, Fangyi; Chan, Jeannie D.; Fang, Ferric C.; Lynch, John B.; Salipante, Stephen J.

doi:10.1126/scitranslmed.adk8222

Cited by 7 publications

(1 citation statement)

References 65 publications

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“…In broilers, the gut microbiota are well characterized within the ceca and have been directly linked to animal health and performance [42]. Recently, it has been discovered in humans that surgical site infections (SSI) are closely connected to a patient's immediate preoperative microbiome [43]. Despite the inherent differences across species, there are grounds to consider the characterization of broiler gut microbial communities and their impacts on BCO lameness etiology and pathogenesis-particularly concerning the concept of a "leaky gut" and its association with infection of leg bone microfractures in a similar fashion to SSIs.…”

Section: Discussionmentioning

confidence: 99%

Assessing the Impact of Spraying an Enterococcus faecium-Based Probiotic on Day-Old Broiler Chicks at Hatch on the Incidence of Bacterial Chondronecrosis with Osteomyelitis Lameness Using a Staphylococcus Challenge Model

Do,

Anthney,

Alharbi

et al. 2024

Animals

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Bacterial chondronecrosis with osteomyelitis (BCO) lameness is a bone disease characterized by the translocation of bacteria from the gastrointestinal tract, which colonize microfractures in broiler leg bones caused by rapid animal growth rate and weight gain, resulting in lameness. As such, BCO lameness represents a significant challenge for the poultry industry. This study aims to evaluate the effect of spraying broiler chicks on d0 at hatch with an Enterococcus faecium probiotic on the incidence of BCO-induced lameness, utilizing a Staphylococcus aureus challenge model. There were four treatments: (1) negative control (no probiotic + no challenge, NC); (2) positive control (no probiotic + challenge, PC); (3) low dosage (4.0 × 108 CFU/chick + challenge, LOW); and (4) high dosage (2.0 × 109 CFU/chick + challenge, HIGH). On d5, groups two through four were challenged with Staphylococcus aureus through the drinking water at a concentration of 1.0 × 105 CFU/mL. Cumulative lameness incidence was determined through daily evaluations and necropsies conducted on lame birds starting from d22. Data were subjected to a binomial general regression analysis (significant p < 0.05). On d56, the PC group exhibited the highest cumulative lameness incidence (58.0%; p < 0.05), followed by LOW (36.0%), HIGH (28.7%), and NC groups (25.3%), respectively. These results suggest early probiotic application at day-of-hatch successfully reduced the incidence of lameness in challenged birds, thus contributing to understanding of efficient and sustainable broiler production.

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Section: Discussionmentioning

confidence: 99%

Assessing the Impact of Spraying an Enterococcus faecium-Based Probiotic on Day-Old Broiler Chicks at Hatch on the Incidence of Bacterial Chondronecrosis with Osteomyelitis Lameness Using a Staphylococcus Challenge Model

Do,

Anthney,

Alharbi

et al. 2024

Animals

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Establishing a role for the oral microbiome in infectious complications following major oral cavity cancer surgery

Gallant,

Vivek,

McKeon

et al. 2024

Oral Oncology

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Similarity of drug targets to human microbiome metaproteome promotes pharmacological promiscuity

Beaudoin,

Norget,

Hala

et al. 2024

Preprint

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Similarity between candidate drug targets and human proteins is commonly assessed to minimize the occurrence of side effects. Although numerous drugs have been found to disrupt the health of the human microbiome, no comprehensive comparison between established drug targets and the human microbiome metaproteome has yet been conducted. Therefore, herein, sequence and structure alignments between human and pathogen drug targets and representative human gut, oral, and vaginal microbiome metaproteomes were performed. Both human and pathogen drug targets were found to be similar in sequence, function, structure, and drug binding capacity to proteins in diverse pathogenic and non-pathogenic bacteria from all three microbiomes. The gut metaproteome was identified as particularly susceptible overall to off-target effects. Certain symptoms, such as infections and immune disorders, may be more common among drugs that non-selectively target host microbiota. These findings suggest that similarities between human microbiome metaproteomes and drug target candidates should be routinely checked.

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Contribution of the patient microbiome to surgical site infection and antibiotic prophylaxis failure in spine surgery

Cited by 7 publications

References 65 publications

Assessing the Impact of Spraying an Enterococcus faecium-Based Probiotic on Day-Old Broiler Chicks at Hatch on the Incidence of Bacterial Chondronecrosis with Osteomyelitis Lameness Using a Staphylococcus Challenge Model

Assessing the Impact of Spraying an Enterococcus faecium-Based Probiotic on Day-Old Broiler Chicks at Hatch on the Incidence of Bacterial Chondronecrosis with Osteomyelitis Lameness Using a Staphylococcus Challenge Model

Establishing a role for the oral microbiome in infectious complications following major oral cavity cancer surgery

Similarity of drug targets to human microbiome metaproteome promotes pharmacological promiscuity

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