Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of dolichols in plants

Skorupińska-Tudek, Karolina; Poznański, Jarosław; Wójcik, Jacek; Bieńkowski, Tomasz; Szostkiewicz, Izabela; Zelman-Femiak, Monika; Bajda, Agnieszka; Chojnacki, Tadeusz; Olszowska, O.; Grünler, Jacob; Meyer, Odile; Rohmer, Michel; Danikiewicz, Witold; Świeżewska, Ewa

doi:10.1016/j.chemphyslip.2008.05.112

Cited by 7 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several published studies support the hypothesis that IPP, GPP, and GGPP are transported between the cytosolic and plastidial compartments, with a preference for transport from the plastid to the cytosol (Hemmerlin et al, 2003;Skorupinska-Tudek et al, 2008). This observation corroborates in vivo observations (Kasahara et al, 2002(Kasahara et al, , 2004Laule et al, 2003;Sakakibara et al, 2005) with inhibitor-treated Arabidopsis seedlings, cell cultures of Madagascar periwinkle (Catharanthus roseus) (Schuhr et al, 2003), and BY-2 cells (Hemmerlin et al, 2003).…”

Section: Discussionsupporting

confidence: 76%

“…To test this hypothesis, transformed BY-2 cells were also treated with Fos at 40 mM, which resulted in a dramatic translocation of GFP-BD-CVIL to the nucleus, with traces of fluorescence localized to the plasma membrane ( Figure 3; see Supplemental Table 1 online). Application of both inhibitors completely abolished integration of GFP-BD-CVIL into the plasma membrane, suggesting that some crosstalk between the pathways must exist, as demonstrated for the biosynthesis of diterpenoid ginkgolides (Schwarz and Arigoni, 1999) and, more recently, for the biosynthesis of dolichols (Skorupinska-Tudek et al, 2008). Nevertheless, the dominant contribution of the MEP pathway to the synthesis of the geranylgeranyl moiety for isoprenylation of the GFP-BD-CVIL reporter protein was clear.…”

Section: In Vivo Inhibition Of Gfp-bd-cvil Geranylgeranylationmentioning

confidence: 97%

See 1 more Smart Citation

The Plastidial 2-C-Methyl-d-Erythritol 4-Phosphate Pathway Provides the Isoprenyl Moiety for Protein Geranylgeranylation in Tobacco BY-2 Cells

et al. 2009

Self Cite

View full text Add to dashboard Cite

Protein farnesylation and geranylgeranylation are important posttranslational modifications in eukaryotic cells. We visualized in transformed Nicotiana tabacum Bright Yellow-2 (BY-2) cells the geranylgeranylation and plasma membrane localization of GFP-BD-CVIL, which consists of green fluorescent protein (GFP) fused to the C-terminal polybasic domain (BD) and CVIL isoprenylation motif from the Oryza sativa calmodulin, CaM61. Treatment with fosmidomycin (Fos) or oxoclomazone (OC), inhibitors of the plastidial 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, caused mislocalization of the protein to the nucleus, whereas treatment with mevinolin, an inhibitor of the cytosolic mevalonate pathway, did not. The nuclear localization of GFP-BD-CVIL in the presence of MEP pathway inhibitors was completely reversed by all-transgeranylgeraniol (GGol). Furthermore, 1-deoxy-D-xylulose (DX) reversed the effects of OC, but not Fos, consistent with the hypothesis that OC blocks 1-deoxy-D-xylulose 5-phosphate synthesis, whereas Fos inhibits its conversion to 2-C-methyl-Derythritol 4-phosphate. By contrast, GGol and DX did not rescue the nuclear mislocalization of GFP-BD-CVIL in the presence of a protein geranylgeranyltransferase type 1 inhibitor. Thus, the MEP pathway has an essential role in geranylgeranyl diphosphate (GGPP) biosynthesis and protein geranylgeranylation in BY-2 cells. GFP-BD-CVIL is a versatile tool for identifying pharmaceuticals and herbicides that interfere either with GGPP biosynthesis or with protein geranylgeranylation.

show abstract

Section: Discussionsupporting

confidence: 76%

Section: In Vivo Inhibition Of Gfp-bd-cvil Geranylgeranylationmentioning

confidence: 97%

The Plastidial 2-C-Methyl-d-Erythritol 4-Phosphate Pathway Provides the Isoprenyl Moiety for Protein Geranylgeranylation in Tobacco BY-2 Cells

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…This "division of labor" allows for the compartment-specific synthesis of brassinosteroids, sesquiterpenes, sterols, and ubiquinone from the cytosolic MVA pathway, while the MEP pathway provides the precursors for the synthesis of abscisic acid, carotenoids, chlorophyll, gibberellins, plastoquinone, and various mono-and diterpenes in the plastid (Lange et al, 2000;Vranová et al, 2013). In spite of this strict compartmentalization of the two pathways, the exchange of isoprenoid precursors between the plastids and cytosol is well recognized in some plant species and leads to the synthesis of "mosaic compounds" that are composed of isoprenoid intermediates derived from both the MVA and MEP pathways (Hemmerlin et al, 2003;Laule et al, 2003;Dudareva et al, 2005;Bartram et al, 2006;Skorupinska-Tudek et al, 2008;May et al, 2013;Opitz et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Polyprenols Are Synthesized by a Plastidial cis-Prenyltransferase and Influence Photosynthetic Performance

et al. 2017

Self Cite

View full text Add to dashboard Cite

Plants accumulate a family of hydrophobic polymers known as polyprenols, yet how they are synthesized, where they reside in the cell, and what role they serve is largely unknown. Using Arabidopsis thaliana as a model, we present evidence for the involvement of a plastidial cis-prenyltransferase (AtCPT7) in polyprenol synthesis. Gene inactivation and RNAi-mediated knockdown of AtCPT7 eliminated leaf polyprenols, while its overexpression increased their content. Complementation tests in the polyprenol-deficient yeast Δrer2 mutant and enzyme assays with recombinant AtCPT7 confirmed that the enzyme synthesizes polyprenols of ;55 carbons in length using geranylgeranyl diphosphate (GGPP) and isopentenyl diphosphate as substrates. Immunodetection and in vivo localization of AtCPT7 fluorescent protein fusions showed that AtCPT7 resides in the stroma of mesophyll chloroplasts. The enzymatic products of AtCPT7 accumulate in thylakoid membranes, and in their absence, thylakoids adopt an increasingly "fluid membrane" state. Chlorophyll fluorescence measurements from the leaves of polyprenol-deficient plants revealed impaired photosystem II operating efficiency, and their thylakoids exhibited a decreased rate of electron transport. These results establish that (1) plastidial AtCPT7 extends the length of GGPP to ;55 carbons, which then accumulate in thylakoid membranes; and (2) these polyprenols influence photosynthetic performance through their modulation of thylakoid membrane dynamics.

show abstract

“…In yeast and mammalian cells, Dol-P is synthesized exclusively from the mevalonic pathway between the cytosol and the ER compartments (Schenk et al, 2001). In plants, the initial steps of Dol-P synthesis operate in the plastids from deoxy xylulose phosphatederived isopentenyl diphosphate (IPP) (40 to 50% of total IPP incorporated) and IPP derived from the cytosolic mevalonic pathway (Skorupinska-Tudek et al, 2008). Subsequently, dolichol P-Man synthase (DPMS) located on the cytoplasmic face of the ER catalyzes the transfer of Man from GDP-Man to Dol-P, and the resulting Dol-P-Man is translocated to the luminal side of the ER membrane by an unknown flippase (Sanyal and Menon, 2010).…”

Section: Introductionmentioning

confidence: 99%

DOLICHOL PHOSPHATE MANNOSE SYNTHASE1 Mediates the Biogenesis of Isoprenyl-Linked Glycans and Influences Development, Stress Response, and Ammonium Hypersensitivity in Arabidopsis

et al. 2011

View full text Add to dashboard Cite

The most abundant posttranslational modification in nature is the attachment of preassembled high-mannose-type glycans, which determines the fate and localization of the modified protein and modulates the biological functions of glycosylphosphatidylinositol-anchored and N-glycosylated proteins. In eukaryotes, all mannose residues attached to glycoproteins from the luminal side of the endoplasmic reticulum (ER) derive from the polyprenyl monosaccharide carrier, dolichol P-mannose (Dol-P-Man), which is flipped across the ER membrane to the lumen. We show that in plants, Dol-P-Man is synthesized when Dol-P-Man synthase1 (DPMS1), the catalytic core, interacts with two binding proteins, DPMS2 and DPMS3, that may serve as membrane anchors for DPMS1 or provide catalytic assistance. This configuration is reminiscent of that observed in mammals but is distinct from the single DPMS protein catalyzing Dol-P-Man biosynthesis in bakers' yeast and protozoan parasites. Overexpression of DPMS1 in Arabidopsis thaliana results in disorganized stem morphology and vascular bundle arrangements, wrinkled seed coat, and constitutive ER stress response. Loss-of-function mutations and RNA interference-mediated reduction of DPMS1 expression in Arabidopsis also caused a wrinkled seed coat phenotype and most remarkably enhanced hypersensitivity to ammonium that was manifested by extensive chlorosis and a strong reduction of root growth. Collectively, these data reveal a previously unsuspected role of the prenyl-linked carrier pathway for plant development and physiology that may help integrate several aspects of candidate susceptibility genes to ammonium stress.

show abstract

Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of dolichols in plants

Cited by 7 publications

References 0 publications

The Plastidial 2-C-Methyl-d-Erythritol 4-Phosphate Pathway Provides the Isoprenyl Moiety for Protein Geranylgeranylation in Tobacco BY-2 Cells

The Plastidial 2-C-Methyl-d-Erythritol 4-Phosphate Pathway Provides the Isoprenyl Moiety for Protein Geranylgeranylation in Tobacco BY-2 Cells

Polyprenols Are Synthesized by a Plastidial cis-Prenyltransferase and Influence Photosynthetic Performance

DOLICHOL PHOSPHATE MANNOSE SYNTHASE1 Mediates the Biogenesis of Isoprenyl-Linked Glycans and Influences Development, Stress Response, and Ammonium Hypersensitivity in Arabidopsis

Contact Info

Product

Resources

About