Contribution of the Electrophoretic Pattern of Cell Envelope Protein to the Taxonomy of Hyphomonas spp.

Abstract

The membrane protein profiles of marine budding bacteria belonging to the genus Hyphomonas were determined and analyzed. Hyphornonas spp. synthesized an unusually large number (four or more) of high-apparent-molecular-weight proteins (apparent molecular weight, 264,000). Species were compared by using a membrane protein profile index developed for this study. Our results supported the division of extant members of this genus into five species. The membrane protein profile index for Hyphomonas hirschiana and Hyphomonas neptunium was higher than suggested by other phenetic characteristics.

Hyphomonas adhaerens sp. nov., Hyphomonas johnsonii sp. nov. and Hyphomonas rosenbergii sp. nov., marine budding and prosthecate bacteria.

“…Hyphomonas species synthesized at least four outermembrane proteins with high apparent molecular masses ( 100 kDa), which is a distinguishing characteristic of this genus (Shen et al, 1989 ; Weiner, 1986). The results for the MPPI supported the division of extant members of this genus into five species (Dagasan & Weiner, 1986). …”

“…MHS-3 T (lanes 10-12) and VP6 T (lanes 5-7) synthesized substantial amounts of a Hyphomonas signature protein (approx. 46-48 kDa ; Dagasan & Weiner, 1986). MHS-2 T (lane 9) had the most unique protein profile and, unlike all the other strains, did not synthesize a very high-copy-number protein.…”

“…MHS-2 T (lane 9) had the most unique protein profile and, unlike all the other strains, did not synthesize a very high-copy-number protein. It did, however, synthesize genus-typical (Dagasan & Weiner, 1986) 31 and 36 kDa proteins. Table 4 shows the serological relatedness of the more recently isolated strains of Hyphomonas to one another and to the extant strains.…”

“…Under defined growth parameters, the membrane protein content of bacteria is characteristic for each species (Dagasan & Weiner, 1986) and therefore serves as a useful taxonomic tool (Vauterin et al, 1993). Hyphomonas species synthesized at least four outermembrane proteins with high apparent molecular masses ( 100 kDa), which is a distinguishing characteristic of this genus (Shen et al, 1989 ; Weiner, 1986).…”

“…Cell envelope purification, membrane protein purification, SDS-PAGE and membrane protein profile index (MPPI) calculations were done as described previously (Dagasan & Weiner, 1986).…”