Contribution of SMC (Structural Maintenance of Chromosomes) and SpoIIIE to Chromosome Segregation in Staphylococci

Yu, Wenchao; Herbert, Silvia; Graumann, Peter L.; Götz, Friedrich

doi:10.1128/jb.00010-10

Cited by 34 publications

(46 citation statements)

References 35 publications

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“…SpoIIIE, a homolog of the Bacillus subtilis (45.9% identity) along with FtsK, operates in independent pathways for correcting chromosome segregation during cell division [40,41]. DivIB is necessary for a molecular checkpoint to guarantee the correct assembly of the bacterial divisome which is a macromolecular machine formed by a variety of proteins [42].…”

Section: Discussionmentioning

confidence: 99%

Isobaric Tags for Relative and Absolute Quantitation Proteomics Analysis of Gene Regulation by SprC in Staphylococcus Aureus

Zhao

Ding

et al. 2017

Future Microbiol.

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Aim:To explore the complete gene networks regulated by small RNA SprC and its targets in Staphylococcus aureus. Materials & methods: The isobaric tags for relative and absolute quantitation and bioinformatic methods were utilized to identify and analyze the target proteins affected by SprC in S. aureus N315. Results: Proteomic analysis showed that the expression of 44 proteins was modulated by SprC. Further, bioinformatic analysis displayed that these affected proteins mainly associated with metabolic and cellular process, biological regulation and catalytic activity. Conclusion: Our data provide a rich resource of SprC targets in S. aureus, although the mechanism of regulation by SprC is yet to be elucidated.

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Section: Discussionmentioning

confidence: 99%

Isobaric Tags for Relative and Absolute Quantitation Proteomics Analysis of Gene Regulation by SprC in Staphylococcus Aureus

Zhao

Ding

et al. 2017

Future Microbiol.

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“…The spoIIIE gene sequence was almost entirely removed from the S. aureus chromosome except for 64 bp at the 3 0 end (Yu et al, 2010) which most likely contain the RBS for the ymfC gene, located downstream of spoIIIE. Deletion of spoIIIE did not alter growth rate (duplication time of both 8325-4DspoIIIE and NCTC8325-4 strains was 28 6 1 min, at 378C in rich medium), but it mildly affected the normal organization and distribution of the chromosomes ( Fig.…”

Section: Lack Of S Aureus Spoiiie Causes Chromosome Condensationmentioning

confidence: 99%

Staphylococcus aureus requires at least one FtsK/SpoIIIE protein for correct chromosome segregation

Veiga

Pinho

2016

Molecular Microbiology

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Summary Faithful coordination between bacterial cell division and chromosome segregation in rod‐shaped bacteria, such as Escherichia coli and Bacillus subtilis, is dependent on the DNA translocase activity of FtsK/SpoIIIE proteins, which move DNA away from the division site before cytokinesis is completed. However, the role of these proteins in chromosome partitioning has not been well studied in spherical bacteria. Here, it was shown that the two Staphylococcus aureus FtsK/SpoIIIE homologues, SpoIIIE and FtsK, operate in independent pathways to ensure correct chromosome management during cell division. SpoIIIE forms foci at the centre of the closing septum in at least 50% of the cells that are close to complete septum synthesis. FtsK is a multifunctional septal protein with a C‐terminal DNA translocase domain that is not required for correct chromosome management in the presence of SpoIIIE. However, lack of both SpoIIIE and FtsK causes severe nucleoid segregation and morphological defects, showing that the two proteins have partially redundant roles in S. aureus.

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“…The proteins that influence chromosome segregation and spatial organization include condensins: proteins of the structural maintenance of chromosomes (SMC) (in B. subtilis, S. coelicolor, Streptococcus pneumoniae, and Staphylococcus aureus) or MukB (in E. coli) families, which generate large-scale compaction of the chromosome (17)(18)(19)(20)(21)(22)(23)(24). Interestingly, the SMC proteins have been shown to be recruited to ParB complexes in B. subtilis and S. pneumoniae, confirming the importance of oriC region compaction for chromosome segregation (25,26).…”

Section: Mers) Varied Numbers Of Pars Sites (From 3 In Vibrio Choleraementioning

confidence: 99%

Topoisomerase I (TopA) Is Recruited to ParB Complexes and Is Required for Proper Chromosome Organization during Streptomyces coelicolor Sporulation

et al. 2013

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c Streptomyces species are bacteria that resemble filamentous fungi in their hyphal mode of growth and sporulation. In Streptomyces coelicolor, the conversion of multigenomic aerial hyphae into chains of unigenomic spores requires synchronized septation accompanied by segregation of tens of chromosomes into prespore compartments. The chromosome segregation is dependent on ParB protein, which assembles into an array of nucleoprotein complexes in the aerial hyphae. Here, we report that nucleoprotein ParB complexes are bound in vitro and in vivo by topoisomerase I, TopA, which is the only topoisomerase I homolog found in S. coelicolor. TopA cannot be eliminated, and its depletion inhibits growth and blocks sporulation. Surprisingly, sporulation in the TopA-depleted strain could be partially restored by deletion of parB. Furthermore, the formation of regularly spaced ParB complexes, which is a prerequisite for proper chromosome segregation and septation during the development of aerial hyphae, has been found to depend on TopA. We hypothesize that TopA is recruited to ParB complexes during sporulation, and its activity is required to resolve segregating chromosomes.

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Contribution of SMC (Structural Maintenance of Chromosomes) and SpoIIIE to Chromosome Segregation in Staphylococci

Cited by 34 publications

References 35 publications

Isobaric Tags for Relative and Absolute Quantitation Proteomics Analysis of Gene Regulation by SprC in Staphylococcus Aureus

Isobaric Tags for Relative and Absolute Quantitation Proteomics Analysis of Gene Regulation by SprC in Staphylococcus Aureus

Staphylococcus aureus requires at least one FtsK/SpoIIIE protein for correct chromosome segregation

Topoisomerase I (TopA) Is Recruited to ParB Complexes and Is Required for Proper Chromosome Organization during Streptomyces coelicolor Sporulation

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