Contribution of quorum sensing to virulence and antibiotic resistance in zoonotic bacteria

Fan, Qingying; Zuo, Jing; Wang, Haikun; Grenier, Daniel; Li, Yang; Wang, Yan

doi:10.1016/j.biotechadv.2022.107965

Cited by 21 publications

(12 citation statements)

References 168 publications

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“…AI-2 QS is a standard QS system regulating drug resistance and virulence in Gram-negative and Grampositive bacteria [10]. AI-2 QS has been reported to up-regulate the expression of e ux pump genes to help Streptococcus suis resist uoroquinolones [25].…”

Section: Discussionmentioning

confidence: 99%

AI-2 quorum sensing system contributes fluoroquinolones resistance of Salmonella typhimurium by regulating metabolism

Gao

Quan

Jin

et al. 2023

Preprint

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The multidrug-resistant Salmonella is becoming major health threat due to inappropriate use of antibiotics. It is found that the metabolic changes contributed to bacterial drug resistance. AI-2 quorum sensing (QS) is involved in the regulation of bacterial metabolic activities. However, the role of AI-2 quorum sensing in the metabolic activities and drug resistance of Salmonella remain unclear. In this study, we determined the drug sensitivity of Salmonella typhimurium by 96-well plate and then determined the time-killing curve. Then, the regulation of AI-2 quorum sensing on Salmonella metabolic activity was analyzed by quantitative real‑time PCR and LC-MS metabonomics. The results showed that luxS mutant strain of Salmonella typhimurium lacking AI-2 quorum sensing was at least 4-fold less sensitive to killing by fluoroquinolone antibiotics. The decreased cell killing after the fluoroquinolones challenge was found to involve a low metabolic state presented by up-regulating anaerobic respiration, and LC-MS metabonomics analysis showed that the low metabolic state of luxS deletion strain might be related to the metabolism of amino acids such as leucine and valine. In conclusion, this study indicated that the deletion of luxS gene in Salmonella typhimurium mediated the tolerance to fluoroquinolones by affecting valine metabolism and up-regulating the anaerobic metabolism pathway.

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Section: Discussionmentioning

confidence: 99%

AI-2 quorum sensing system contributes fluoroquinolones resistance of Salmonella typhimurium by regulating metabolism

Gao

Quan

Jin

et al. 2023

Preprint

Self Cite

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“…(i) The bacterial number of individual isolates was lower than in the gnotobiotic setting. Many bacterial virulence genes are dependent on quorum-sensing, and individual bacteria do not reach high enough numbers to express virulence factors ( 75 , 76 ). (ii) Enterobacter and Pseudomonas present in the community provide essential nutrients like riboflavin to larvae ( 11 ), which helps their development, making their immune system more competent and resulting in lower mortality.…”

Section: Discussionmentioning

confidence: 99%

Phage Therapy for Mosquito Larval Control: a Proof-of-Principle Study

Tikhe

Dimopoulos

2022

mBio

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“…EPS can separate colonies and form a pathway for transporting metabolites and nutrients. In addition, EPS can maintain the biofilm’s structure and contribute to bacteria colonization on the non-biological surface ( Fan et al, 2022 ). Curli fimbriae and cellulose, whose products are regulated by csgD , are common EPS components found in bacterial biofilms ( Dieltjens et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Novel quorum sensing inhibitor Echinatin as an antibacterial synergist against Escherichia coli

et al. 2022

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A new antibacterial strategy based on inhibiting bacterial quorum sensing (QS) has emerged as a promising method of attenuating bacterial pathogenicity and preventing bacterial resistance to antibiotics. In this study, we screened Echinatin (Ech) with high-efficiency anti-QS from 13 flavonoids through the AI-2 bioluminescence assay. Additionally, crystal violet (CV) staining combined with confocal laser scanning microscopy (CLSM) was used to evaluate the effect of anti-biofilm against Escherichia coli (E. coli). Further, the antibacterial synergistic effect of Ech and marketed antibiotics were measured by broth dilution and Alamar Blue Assay. It was found that Ech interfered with the phenotype of QS, including biofilm formation, exopolysaccharide (EPS) production, and motility, without affecting bacterial growth and metabolic activity. Moreover, qRT-PCR exhibited that Ech significantly reduced the expression of QS-regulated genes (luxS, pfs, lsrB, lsrK, lsrR, flhC, flhD, fliC, csgD, and stx2). More important, Ech with currently marketed colistin antibiotics (including colistin B and colistin E) showed significantly synergistically increased antibacterial activity in overcoming antibiotic resistance of E. coli. In summary, these results suggested the potent anti-QS and novel antibacterial synergist candidate of Ech for treating E. coli infections.

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Contribution of quorum sensing to virulence and antibiotic resistance in zoonotic bacteria

Cited by 21 publications

References 168 publications

AI-2 quorum sensing system contributes fluoroquinolones resistance of Salmonella typhimurium by regulating metabolism

AI-2 quorum sensing system contributes fluoroquinolones resistance of Salmonella typhimurium by regulating metabolism

Phage Therapy for Mosquito Larval Control: a Proof-of-Principle Study

Novel quorum sensing inhibitor Echinatin as an antibacterial synergist against Escherichia coli

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