Contribution of phosphoglucosamine mutase to determination of bacterial cell morphology in Streptococcus gordonii

Abstract

Phosphoglucosamine mutase (GlmM; EC 5.4.2.10) catalyzes the interconversion of glucosamine-6-phosphate to glucosamine-1-phosphate, an essential step in the biosynthetic pathway leading to the formation of the peptidoglycan precursor uridine 5'-diphospho-N-acetylglucosamine. We have recently identified the gene (glmM) encoding the enzyme of Streptococcus gordonii, an early colonizer on the human tooth and an important cause of infective endocarditis, and indicated that the glmM mutation in S. gordonii appears to influence bacterial cell growth, morphology, and sensitivity to penicillins. Moreover, the glmM mutation results in increased sensitivity to polymorphonuclear leukocyte (PMN)-dependent killing. In the present study, we observed similarities in the utilization of sugar between the wild-type strain and the glmM mutant of S. gordonii when cultivated with medium containing 0.2% glucose, fructose, lactose, or sucrose. Morphological analyses clearly indicated that the glmM mutation causes marked elongation of the streptococcal chains, enlargement of bacterial cells, increased distortion of the bacterial cell surface, and defects in cell separation. These results suggest that mutations in glmM appear to influence bacterial cell growth and morphology, independent of the carbon source.

Chitin degradation potential and whole-genome sequence of Streptomyces diastaticus strain CS1801

“…β-Galactosidase and β-glucosidase split the β-(1,4)-glycosidic bond of (GlcN) 2 to generate GlcN (Chinchetru et al 1989;Sorokin et al 2014 (Leriche et al 1997). Then, 6-phosphate-GlcN is transformed to GlcN-1-phosphate by phosphoglucosamine mutase (Shimazu et al 2012). N-GlcNAc-1-phosphate is formed by GlcN-1-phosphate N-acetyltransferase, which transforms GlcN-1-phosphate to N-GlcNAc-1-phosphate (Zhang et al 2015).…”

Comparative transcriptional profiling of tildipirosin-resistant and sensitive Haemophilus parasuis

“…Another key upregulated gene glmM (2.6-fold change), encoding phosphoglucosamine mutase, has been demonstrated to contribute to the resistance of Streptococcus , and is the drug target for regulating resistance. In addition, glmM is directly upstream of a multiple repeat polypeptide essential for the expression of methicillin resistance in Staphylococcus aureus 41–44 . The other upregulated genes have not been reported, but also might contribute to regulate metabolic pathways related to bacterial resistance to tildipirosin which were in need of verification in the future.

Figure 8The differential expression on relative mRNA abundance of Ten genes in JS32 and HB32 compared with JS0135 .

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