Contribution of pathogenic fungi to N2O emissions increases temporally in intensively managed strawberry cropping soil

Huang, Ying; Jing, Jinquan; Yan, Meiling; Hazard, Christina; Chen, Yuehong; Guo, Chengbao; Xu, Xiao; Lin, Jiujun

doi:10.1007/s00253-021-11163-8

Cited by 10 publications

(3 citation statements)

References 53 publications

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“…Additionally, we observed reduced fungal abundance in the liming treatment. Fungi contribute to N 2 O emissions (Aldossari and Ishii, 2021), and the ratio of fungi to bacteria is usually higher in acidic soils, potentially leading to increased fungus-mediated N 2 O emissions (Chen et al, 2015; Huang et al, 2021; Lourenço et al, 2018; Rütting et al, 2013). The reduced fungal abundance in the liming treatment may have contributed to the lack of a significant difference in N 2 O emissions despite the increased abundances of nitrifiers and denitrifiers.…”

Section: Discussionmentioning

confidence: 99%

Liming enhances the abundance and stability of nitrogen-cycling microbes: The buffering effect of long-term lime application

Mitsuta,

Lourenco,

Chang

et al. 2024

Preprint

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Lime application (liming) has historically been used to ameliorate soil acidity in grasslands. Liming effectively improves soil pH, plant productivity, and soil physicochemical properties, but the long-term impact of acidity control by liming on key microbial nitrogen (N)-cycling genes in semi-natural grasslands is unknown. We investigated the effect of 65 years of liming on N-cycling processes in the limed and control plots of the Ossekampen long-term grassland experiment in the Netherlands. These plots have not received any other fertilizers for 65 years. Soil sampling and nitrous oxide (N2O) emission measurements were conducted three times in spring and four times in summer, and quantitative real-time PCR was performed to determine the abundances of N-cycling genes, including ammonia-oxidation (amoA), denitrification (nirS, nirK, nosZ), and N-fixation (nifH) genes. Long-term liming increased the abundances of nitrifiers and denitrifiers but did not increase N2O emissions. Additionally, liming had a buffering effect that stabilized the population of N-cycling microbes against seasonal variations in abundance. Our results indicate that improving soil acidity through liming facilitates microbial N-cycling processes without increasing N2O emissions.

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Section: Discussionmentioning

confidence: 99%

Liming enhances the abundance and stability of nitrogen-cycling microbes: The buffering effect of long-term lime application

Mitsuta,

Lourenco,

Chang

et al. 2024

Preprint

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“…The composting temperature, pH, moisture, electrical conductivity (EC), total nitrogen (TN), total organic carbon (TOC), inorganic N (NH 4 + -N, NO 3 − -N), protease and ammonification rate were measuring following the procedures stated previously in Huang et al ( 2019a ). The N 2 O was determined using an Agilent 7890A gas chromatograph (Agilent, Beijing, China) (Huang et al 2021 ). The concentration of NH 3 was determined by absorbing NH 3 with 2% boric acid and applying the titrimetric method as described by Cui et al ( 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Hyperthermophilic pretreatment composting can reduce ammonia emissions by controlling proteolytic bacterial community and the physicochemical properties

Huang¹,

Chen²,

Huang³

et al. 2023

Bioresour. Bioprocess.

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Proteolysis is the rate-limiting step in the mineralization of organic nitrogen into ammonium (NH4+) and thereby the ammonia (NH3) released during the composting. However, the dynamics of bacterial proteolytic communities related to NH3 emissions during the composting systems are mostly unknown. This study aimed to examine and compare the effects of hyperthermophilic pretreatment composting (HPC) and traditional composting (TC) methods on (i) the difference of NH3 loss and nitrogenous compounds; (ii) the dynamics of the proteolytic bacterial community involved in the proteolysis and (iii) the correlation between the proteolytic bacterial community, biophysiochemical characteristics and NH3 loss. Results revealed that the HPC decreased NH3 loss by 42% as compared to TC during 60-day composting period. This was accompanied with an inhibitory effect on protease activity in the HPC where the relative abundances of the proteolytic bacteria (Bacillus megaterium and Staphylococcus cohnii) were reduced significantly as compared to TC. Partial least-squares path modeling suggested that various physicochemical properties such as higher temperature as well as lower C/N ratio during composting played a dominant role in affecting the abundance of proteolytic bacteria, which may have been an important factor contributing to the lower NH3 loss in HPC. All these findings lead us to conclude that the HPC can significantly reduce NH3 loss by inhibiting the proteolytic bacteria and protease activity responsible for NH3 release. Graphical Abstract

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“…At present, facility cultivation has become the main cultivation pattern of strawberries as it is not limited by the external environment [4]. However, strawberries are usually planted continuously in the same field of the facility, resulting in the spread of soil pathogens, soil quality degradation, and nutrient imbalance [5,6]. If there is a lack of timely and effective control, soil-borne diseases can easily develop, resulting in reduced strawberry yield and quality [7].…”

Section: Introductionmentioning

confidence: 99%

Effects of Soil Amendments on Soil Properties, Soil-Borne Pathogens, and Strawberry Growth after Dazomet Fumigation

Li,

Andom,

Fang

et al. 2023

Agriculture

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Soil fumigation can kill soil pathogens and solve the problem of crop continuous cropping. However, soil fumigation also has negative effects on the soil environment. One way to mitigate the negative effects is to apply soil amendments, but there is limited evidence of the effects of combining soil fumigation and amendments. This study was a controlled environmental pot trial. We measured the effects of dazomet fumigation combined with soil amendments on soil-borne pathogens, soil nutrients, enzyme activities, and strawberry growth. The results showed that dazomet fumigation combined with soil amendments significantly increased the content of ammonium nitrogen, available phosphorus and organic matter and increased soil activities by varying degrees. We also found that the control effect of soil-borne pathogens Fusarium spp. and Phytophthora spp. was further enhanced, reaching 88.97–96.88%. Correlation analysis showed that the growth indices of strawberries such as plant height, stem diameter, chlorophyll content, and fresh weight were negatively correlated with Fusarium spp. (R = −0.75, R= −0.62, R = −0.71, R = −0.88; p < 0.01) and Phytophthora spp. (R = −0.72, R= −0.72, R = −0.78, R = −0.91; p ≤ 0.001), respectively. The effect of fumigation combined with soil amendments was better than that of fumigation alone, and silicon fertilizer had the best effect. Our study suggests that dazomet fumigation combined with soil amendments can improve soil nutrient supply, activate soil enzyme activities, enhance the control effect of soil-borne pathogens, and thus promote strawberry growth.

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Contribution of pathogenic fungi to N2O emissions increases temporally in intensively managed strawberry cropping soil

Cited by 10 publications

References 53 publications

Liming enhances the abundance and stability of nitrogen-cycling microbes: The buffering effect of long-term lime application

Liming enhances the abundance and stability of nitrogen-cycling microbes: The buffering effect of long-term lime application

Hyperthermophilic pretreatment composting can reduce ammonia emissions by controlling proteolytic bacterial community and the physicochemical properties

Effects of Soil Amendments on Soil Properties, Soil-Borne Pathogens, and Strawberry Growth after Dazomet Fumigation

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